Application of C-banding in twoArachiswild species,Arachis PintoiKrapov. and W.C. Gregory andA. villosulicarpaHoehne to mitotic chromosome analyses

Pierozzi, Neiva Izabel; Galgaro, Maria Leticia; Lopes, Catalina Romero

doi:10.1080/00087114.2001.10589249

Cited by 9 publications

(10 citation statements)

References 30 publications

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“…The scarcity of chromosome banding patterns does not often allow for the precise identification of chromosomes in karyotypes of the small chromosome plant species (Pierozzi et al 2001;Vanzela et al 2002). We encountered a similar problem at the beginning of our investigation of the Linum chromosome.…”

Section: Discussionmentioning

confidence: 97%

Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis

et al. 2008

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Karyotypes of species sects. Linum and Adenolinum have been studied using C/DAPI-banding, Ag-NOR staining, FISH with 5S and 26S rDNA and RAPD analysis. C/DAPI-banding patterns enabled identification of all homologous chromosome pairs in the studied karyotypes. The revealed high similarity between species L. grandiflorum (2n = 16) and L. decumbens by chromosome and molecular markers proved their close genome relationship and identified the chromosome number in L. decumbens as 2n = 16. The similarity found for C/DAPI-banding patterns between species with the same chromosome numbers corresponds with the results obtained by RAPD-analysis, showing clusterization of 16-, 18- and 30-chromosome species into three separate groups. 5S rDNA and 26S rDNA were co-localized in NOR-chromosome 1 in the genomes of all species investigated. In 30-chromosome species, there were three separate 5S rDNA sites in chromosomes 3, 8 and 13. In 16-chromosome species, a separate 5S rDNA site was also located in chromosome 3, whereas in 18-chromosome species it was found in the long arm of NOR-chromosome 1. Thus, the difference in localization of rDNA sites in species with 2n = 16, 2n = 30 and 2n = 18 confirms taxonomists opinion, who attributed these species to different sects. Linum and Adenolinum, respectively. The obtained results suggest that species with 2n = 16, 2n = 18 and 2n = 30 originated from a 16-chromosome ancestor.

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Section: Discussionmentioning

confidence: 97%

Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis

et al. 2008

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“…The secondary constriction (SC) of mitotic chromosomes, however, was not C-banded. The absence of C-band formation in SC has already been reported in some other plants, e.g., Arachis and Coffea species (Pierozzi et al 2001(Pierozzi et al , 2012, which have chromosomes as small as ramie.…”

Section: Discussionmentioning

confidence: 90%

“…An adapted C-banding technique (Pierozzi et al 2001) was applied for heterochromatin localization in both mitotic and in meiotic pachytene and diakinesis bivalent chromosomes, and NOR-banding using an aqueous solution of 50% AgNO 3 (silver nitrate) and gelatin, as recommended by Howell and Black (1980), was conducted for localization of nucleolar organizer regions (NOR) in mitotic chromosomes.…”

Section: Cytological Preparationsmentioning

confidence: 99%

Karyotype Analysis Using C- and NOR-Banding in Ramie [Boehmeria nivea (L.) Gaud.]

Pierozzi

Baroni

2014

CYTOLOGIA

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Summary Acetic orcein as well as C-and NOR-banding (silver staining) techniques were applied to the mitotic chromosomes of ramie Boehmeria nivea var. nivea (L.) Gaudisch cv. Miyasaki for chromosome characterization. C-banding was also applied in pachytene and diakinesis meiotic phases for heterochromatin localization. The species showed a predominance of submetacentric chromosomes as observed in karyotype formula: 1m + 12sm + 1sm s , as well as a moderate karyotype asymmetry as revealed by the Stebbins criteria and Romero-Zarco and Paszko indices. C-bands were preferentially localized in the chromosome short arms of the mitotic chromosomes. Large blocks of pericentromeric heterochromatin C-bands were seen at pachytene meiotic phase, while at diakinesis phase, C-bands were located on the chromosome ends as well as nearby or on the chiasmata. The interphase nuclei of mitotic cells were classified as prochromosome type, which is the same as S2 type. One pair of chromosomes with satellite segment was seen in the genotype studied after acetic orcein and silver staining (NOR-band). Chromosome morphometric data along with NOR localization were suitable to characterize the Miyasaki cultivar.

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“…It is known that in such plants heterochromatin is mainly localized in centromeric regions, and less frequently, in the intercalary and telomeric regions [35,[38][39][40][41]. Comparative analysis of chromosomes of the forms under study revealed their C banding polymorphism.…”

Section: Discussionmentioning

confidence: 99%

Comparative cytogenetic study of the forms of Macleaya cordata (Willd.) R. Br. From different localities

et al. 2012

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A comparative cytogenetic study of two introduced forms of Makleaya cordata (Willd.) R. Br. = syn. Bocconia cordata Willd. grown in different ecological and geographical regions (Moscow and Donetsk areas) was carried out. In the study, a complex of methods utilizing various chromosomal markers, i.e., C and DAPI banding technique, fluorescence in situ hybridization (FISH) with probes of 26S and 5S rDNA, as well as estimation of the total area of C positive regions (C HCH) in prophase nucleoli and meiosis anal ysis, was used. In the karyotypes (2n = 20), each chromosome was identified on the basis of C banding and FISH patterns and the chromosome ideograms were built. Pericentrometric and telomeric C positive bands in chromosomes of the Moscow form karyotype were found to be smaller and intercalary bands, larger than the corresponding bands in the M. cordata form grown in Donetsk. It was found that the content of C HCH in prophase nucleoli in the form of M. cordata grown in Donetsk was higher than in the form grown in Mos cow. In both forms sites of 26S rDNA and 5s rDNA were localized on satellite chromosome 1 and on chro mosome 4 respectively but the signals were more intensive in the plant form grown in Donetsk. The results of this study enable selecting M. cordata forms for use in pharmacology and recommending them for cultivation in various ecological and geographical regions.

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Application of C-banding in twoArachiswild species,Arachis PintoiKrapov. and W.C. Gregory andA. villosulicarpaHoehne to mitotic chromosome analyses

Cited by 9 publications

References 30 publications

Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis

Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis

Karyotype Analysis Using C- and NOR-Banding in Ramie [<i>Boehmeria nivea</i> (L.) Gaud.]

Comparative cytogenetic study of the forms of Macleaya cordata (Willd.) R. Br. From different localities

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