Application Of Bismuth-film Modified Glassy Carbon Electrode For Solid-phase Extraction Of Sudan I

Cai, Jie; Tu, Yuanqiang; Feng, Guanwen; Huang, Chunyan

doi:10.5185/amlett.2011.4237

Cited by 8 publications

(4 citation statements)

References 1 publication

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“…Then bismuth layer was deposited electrochemically by immersing the modified electrode into a solution containing 0.1 M acetate buffer solution and 100 μL of bismuth ions, and a negative potential for 100 s was applied. 25 Electrochemical measurements including DPV, clean electrode, cyclic votammetry (CV), and electrochemical impedance spectroscopy (EIS) were performed using an electrochemical analyzer (CHI Inc., Shanghai, China) and a three-electrode system at room temperature. All experiments were carried out at room temperature, and all measurements were replicated three times.…”

Section: Tri(2-carboxyethyl)p H O S P H I N E H Y D R O C H L O R I D...mentioning

confidence: 99%

Electrochemical Genosensor To Detect Pathogenic Bacteria (Escherichia coli O157:H7) As Applied in Real Food Samples (Fresh Beef) To Improve Food Safety and Quality Control

Abdalhai

Fernandes

Xia

et al. 2015

J. Agric. Food Chem.

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The electrochemical genosensor is one of the most promising methods for the rapid and reliable detection of pathogenic bacteria. In a previous work, we performed an efficient electrochemical genosensor detection of Staphylococcus aureus by using lead sulfide nanoparticles (PbSNPs). As a continuation of this study, in the present work, the electrochemical genosensor was used to detect Escherichia coli O157:H7. The primer and probes were designed using NCBI database and Sigma-Aldrich primer and probe software. The capture and signalizing probes were modified by thiol (SH) and amine (NH2), respectively. Then, the signalizing probe was connected using cadmium sulfide nanoparticles (CdSNPs), which showed well-defined peaks after electrochemical detection. The genosensor was prepared by immobilization of complementary DNA on the gold electrode surface, which hybridizes with a specific fragment gene from pathogenic to make a sandwich structure. The conductivity and sensitivity of the sensor were increased by using multiwalled carbon nanotubes (MWCNT) that had been modified using chitosan deposited as a thin layer on the glass carbon electrode (GCE) surface, followed by a deposit of bismuth. The peak currents of E. coli O157:H7 correlated in a linear fashion with the concentration of tDNA. The detection limit was 1.97 × 10(-14) M, and the correlation coefficient was 0.989. A poorly defined current response was observed as the negative control and baseline. Our results showed high sensitivity and selectivity of the electrochemical DNA biosensor to the pathogenic bacteria E. coli O157:H7. The biosensor was also used to evaluate the detection of pathogen in real beef samples contaminated artificially. Compared with other electrochemical DNA biosensors, we conclude that this genosensor provides for very efficient detection of pathogenic bacteria. Therefore, this method may have potential application in food safety and related fields.

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Section: Tri(2-carboxyethyl)p H O S P H I N E H Y D R O C H L O R I D...mentioning

confidence: 99%

Electrochemical Genosensor To Detect Pathogenic Bacteria (Escherichia coli O157:H7) As Applied in Real Food Samples (Fresh Beef) To Improve Food Safety and Quality Control

Abdalhai

Fernandes

Xia

et al. 2015

J. Agric. Food Chem.

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“…Almost all methods of determining metal in very small quantities require a considerable amount of time in the pre-concentration stage before measurement. In the DPAdSV method, the pre-concentration stage is shorter, generally less than 1 minute [26][27][28][29]. so the DPAdSV method is very suitable for metal determination in seawater ex-bauxite mines.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Pb Adsorption from Seawater from Former Bauxite Mines Using Crab Shell Waste

Pardi

Fitriyah

Silitonga

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Crab shell waste can cause pollution to the surrounding environment, but has the potential to be used as a biosorbent in the absorption of heavy metals, especially Pb because crab shells contain CaCO3 and chitin. Methods: Several variables that play a role in the biosorption process, among others: initial metal concentration, biosorbent mass, and contact time. Pb metal was determined by the Atomic Absorption Spectroscopy (AAS) method. Results: The results showed that the crab shell biosorbent contained Pb 0.019 mg/g. The concentration of Pb in the shell was used as a correction factor. Optimal result for parameter of initial concentration of Pb is 100 mg/L with efficiency percentage (99.11 ± 0.02)% and adsorption capacity (620.15 ± 0.3) ug/g. The optimal result of contact time is 120 minutes with efficiency percentage (91.22 ± 0.13)%. The optimal yield of biosorbent mass based on the percentage efficiency (98.01 ± 0.2)% is 2 grams. Conclusion: This study can show that crab shell waste can be used as an effective biosorbent for Pb metal adsorption. The results of the optimization of the best factors to be used in the biosorption process of Pb metal were the initial concentration of 100 mg/L, the mass of the biosorbent was 3 grams and the contact time was 110 minutes. The optimum conditions for Pb adsorption were successfully applied to seawater from the former bauxite mine.

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“…The use of nanomaterials and nanotracers in biosensors allows new transduction technology and application of a bismuth layer on glass carbon electrodes to detect heavy metals in samples showing an alternative material to substitute toxic mercury. ,− The main advantages of bismuth are the high capacity to form fused alloys with metals, which are less affected by dissolved oxygen. However, it fails to detect low amounts of analyte due to its poor conductivity, , but applied multiwall carbon nanotubes–bismuth modified on glassy carbon electrodes showed increased sensitivity in the detection of trace metals.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Detection of Foodborne Pathogenic Bacteria (Staphylococcus aureus) Using an Electrochemical DNA Genomic Biosensor and Its Application in Fresh Beef

Abdalhai

Fernandes

Bashari

et al. 2014

J. Agric. Food Chem.

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Rapid early detection of food contamination is the main key in food safety and quality control. Biosensors are emerging as a vibrant area of research, and the use of DNA biosensor recognition detectors is relatively new. In this study a genomic DNA biosensor system with a fixing and capture probe was modified by a sulfhydryl and amino group, respectively, as complementary with target DNA. After immobilization and hybridization, the following sandwich structure fixing DNA-target DNA-capture DNA-PbS NPs was formed to detect pathogenic bacteria (Staphylococuus aureus EF529607.1) by using GCE modified with (multiwalled carbon nanotubes-chitosan-bismuth) to increase the sensitivity of the electrode. The modification procedure was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The sandwich structure was dissolved in 1 M nitric acid to become accessible to the electrode, and the PbS NPs was measured in solution by differential pulse voltammetry (DPV). The results showed that the detection limit of the DNA sensor was 3.17 × 10(-14) M S. aureus using PbS NPs, whereas the result for beef samples was 1.23 ng/mL. Thus, according to the experimental results presented, the DNA biosensor exhibited high sensitivity and rapid response, and it will be useful for the food matrix.

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Application Of Bismuth-film Modified Glassy Carbon Electrode For Solid-phase Extraction Of Sudan I

Cited by 8 publications

References 1 publication

Electrochemical Genosensor To Detect Pathogenic Bacteria (Escherichia coli O157:H7) As Applied in Real Food Samples (Fresh Beef) To Improve Food Safety and Quality Control

Electrochemical Genosensor To Detect Pathogenic Bacteria (Escherichia coli O157:H7) As Applied in Real Food Samples (Fresh Beef) To Improve Food Safety and Quality Control

Optimization of Pb Adsorption from Seawater from Former Bauxite Mines Using Crab Shell Waste

Rapid and Sensitive Detection of Foodborne Pathogenic Bacteria (Staphylococcus aureus) Using an Electrochemical DNA Genomic Biosensor and Its Application in Fresh Beef

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