Application of Bacillus species for controlling root-knot nematode Meloidogyne incognita in eggplant

El-Nagdi, W. M. A.; Abd-El-Khair, H.

doi:10.1186/s42269-019-0187-6

Cited by 15 publications

(12 citation statements)

References 9 publications

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“…In vitro B. velezensis BZR 86 caused 98% mortality of M. incognita J2 following a 24-h exposure to a 9 × 10 7 CFU mL −1 bacterial density [ 55 ]. In our study the nematicidal effect of B. velezensis BZR 86 was also confirmed on cucumber and tomato in soil, in agreement with previous studies that reported a significant suppressiveness of Bacillus strains on root-knot nematode eggs, J2 and root galls on cucumber [ 10 , 55 ], tomato [ 16 , 17 , 53 , 58 ], eggplant [ 15 , 59 ] and hendi [ 60 ]. The mechanisms underlying the RKN suppression by Bacillus strains can be different.…”

Section: Discussionsupporting

confidence: 91%

Selection of Bacterial Strains for Control of Root-Knot Disease Caused by Meloidogyne incognita

et al. 2021

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Root-knot disease caused by Meloidogyne incognita leads to significant crop yield losses that may be aggravated by the association with pathogenic fungi and bacteria. Biological agents can be effectively used against the complex disease of root-knot nematode and pathogenic fungi. In this study, 35 bacterial strains were analyzed for their in vitro nematicidal, antagonistic and growth stimulation activities. Based on results from the in vitro assays, grow-box experiments on tomato and cucumber were carried out with the strain BZR 86 of Bacillus velezensis applied at different concentrations. Effects of B. velezensis BZR 86 on the development of root-knot disease were evaluated by recording root gall index, number of galls and number of eggs in egg masses. Application of B. velezensis BZR 86 noticeably decreased the development of root-knot disease on tomato and cucumber plants, as well as significantly increased growth and biomass of cucumber plants in accordance with bacterial concentration. This study seems to demonstrate that strain B. velezensis BZR 86 could be an additional tool for an environmentally safe control of root-knot disease on horticultural crops.

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Section: Discussionsupporting

confidence: 91%

Selection of Bacterial Strains for Control of Root-Knot Disease Caused by Meloidogyne incognita

et al. 2021

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“…However, it seems that the higher dosage applied the lowest the population (Table 4). Similar finding reported by El-Nagdi and Abd-El-Khair [13] that Meloidogyne spp. population with 30 ml Bt suspension treatment were195 in root and 407 in rhizosphere.…”

Section: Meloidogyne Spp Populationsupporting

confidence: 91%

“…Researchers reported that Bt has strong effect on Meloidogyne sp. mortality [7,13,14]. Reports on the use of Bt to control Meloidogyne spp.…”

Section: Introductionmentioning

confidence: 99%

The Application of Bacillus thuringiensis to Treat Root Knots on Pogostemon cablin Benth

Chamzurni

Savira

Oktarina

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Root knots, caused by Meloidogyne spp. is among the plant pathogens that lead to significant yield loss in patchouli plants. Farmers have used synthetic nematicides to control the pathogen for many years. In fact, the chemicals not only harm the environment but also might degrade quality of agriculture products. Therefore, in this study, we examined several dosages of Bacillus thuringiensis (Bt) to control Meloidogyne spp. on Pogostemon cablin Benth. Three dosages of Bt suspension (30, 40, and 50 ml/plant) were applied to the rhizosphere to observe the incubation period, disease intensity, number of galls, and the population of nematode. The results show that 40 ml/plant of Bt significantly prolongs the incubation time, reduces the disease intensity, and the number of root-knots. None of the dosages tested showed a significant impact on the nematode population. This study showcases the potential of Bt to control Meloidogyne spp. on patchouli plants. More research is required to confirm the efficacy of this approach.

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“…So far, some secondary metabolites can also trigger an immune reaction in the host plants, which leads to systemic resistance (Choudhary and Johri, 2009;Adam et al, 2014), rendering the plant less susceptible to pathogen infection. Some examples also point to enhanced beneficial effects combining both the plant growth attributes and their effect on nematodes control after coinoculation of different rhizobacteria species (Ansari and Ahmad, 2019;El-Nagdi and Abd-El-Khair, 2019;Topalovicé t al., 2020). Plant growth promoting rhizobacteria (PGPR; Lim and Kim, 2009) facilitate plant development by producing phytohormones (auxins, cytokines, ethylene, abscisic acid, etc.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of Meloidogyne spp. in Solanum lycopersicum using a dual combination of Bacillus strains

et al. 2023

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Root-knot nematodes (RKNs, Meloidogyne spp.) are obligate plant parasites that constitute a significant pest for agriculture worldwide. They penetrate the plant roots, reducing the uptake of water and nutrients, causing a significant impact on crop yield. One alternative on focus now for nematode management is biological control. Rhizobacteria within the Bacillus genus show multiple modes of action against plant-parasitic nematodes (PPNs) that can act alone or in combination. In this context, we evaluated a dual-strain bacteria combination (B. paralicheniformi FMCH001 and B. subtilis FMCH002) to reduce nematode infection in tomato plants. We evaluated mortality of larvae from Meloidogyne javanica in vitro, as well as eggs hatching after the treatment. Atraction, penetration, establishment, and reproduction assays in vitro or in pots in tomato plants infected with M. javanica and treated/ untreated with the dual-strain bacteria combination were also performed. Additionally, morphometric parameters comparing giant cells size from galls of treated and untreated plants by using confocal microscopy were also measured. The results showed that this combination of strains has nematicidal properties in the pre-infection phase by decreasing the egg-hatching, juvenile survival, and attractiveness to the roots. Furthermore, nematode establishment, gall formation, and, remarkably, giant cell development was severely impaired after the bacterial treatment, suggesting interference with morphogenetic mechanisms induced by the nematode during GCs development within the plant. Nematode reproduction in tomato plants was reduced independently of the application mode in soil, before or after bacterial treatment. The dual-strain combination was also effective against other PPNs (i.e. Pratylenchus spp.) and in different crops (soybean). Therefore, combining B. paralicheniformis FMCH001 and B. subtilis FMCH002 is an efficient agent for the biological control of Meloidogyne spp. by interfering with different stages of the nematode cycle as a result of multiple modes of action.

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Application of Bacillus species for controlling root-knot nematode Meloidogyne incognita in eggplant

Cited by 15 publications

References 9 publications

Selection of Bacterial Strains for Control of Root-Knot Disease Caused by Meloidogyne incognita

Selection of Bacterial Strains for Control of Root-Knot Disease Caused by Meloidogyne incognita

The Application of Bacillus thuringiensis to Treat Root Knots on Pogostemon cablin Benth

Biocontrol of Meloidogyne spp. in Solanum lycopersicum using a dual combination of Bacillus strains

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