Application of Asymetrical and Hoke Designs for Optimization of Laccase Production by the White-Rot Fungus Fomes fomentarius in Solid-State Fermentation

Abstract

Statistical approaches were employed for the optimization of different cultural parameters for the production of laccase by the white rot fungus Fomes fomentarius MUCL 35117 in wheat bran-based solid medium. first, screening of production parameters was performed using an asymmetrical design 2533//16, and the variables with statistically significant effects on laccase production were identified. Second, inoculum size, CaCl2 concentration, CuSO4 concentration, and incubation time were selected for further optimization studies using a Hoke design. The application of the response surface methodology allows us to determine a set of optimal conditions (CaCl2, 5.5 mg/gs, CuSO4, 2.5 mg/gs, inoculum size, 3 fungal discs (6 mm Ø), and 13 days of static cultivation). Experiments carried out under these conditions led to a laccase production yield of 150 U/g dry substrate.

Screening and Statistical Optimization of Physiochemical Parameters for the Production of Xylanases from Agro-Industrial Wastes

“…Maximum xylanase activity (18.2 ± 2 IU/mL) was attained when substrates were inoculated with 3 mL of freshly prepared inoculum of F. fomentarius and incubated at 35˚C. A white rot fungus revealed different growth and enzyme activity patterns on different substrates with the addition of some nutrient supplements and results were attained [12].…”

Optimised amylases extraction from oat seeds and its impact on bread properties

“…In this paper, the canonical analysis is used also to find out the best experimental conditions, which permitted the maximisation of the amylase production yield. It consists in rewriting the fitted second-degree equation in a form in which it can be more readily understood by a rotation of axes that remove all cross-product terms b jk , X j , X k while keeping the initial origin at the centre point [27]. This step is suitable when the stationary point is outside of the experimental domain.…”

“…When this point is situated outside the experimental domain, the canonical analysis requires only a rotation of the X j axes in such a way that they become parallel to the principal axes Z j of the contour system [27]. Under these conditions, the canonical model is of the form:…”

“…The three replicates at the centre point (run nos [25][26][27] are carried out in order to estimate the pure error variance and the significance of the fitted model was tested by the mean of the analysis of variance (ANOVA) [17,19,23]. Thus, a statistical test of the model fit is made by comparing the variance due to the lack of fit to the pure error variance using the F-test.…”

Enhancement of Laccase Production from Pleurotus ostreatus PVCRSP-7 by altering the Nutritional Conditions using Response Surface Methodology

“…They catalyze the oxidation of a broad range of organic and inorganic substrates, such as phenols, non-phenols, aromatic amines, and their derivatives. These enzymes have the ability to reduce molecular oxygen to water (Bhattacharya et al 2011;Neifar et al 2011;Riva 2006) Laccases have great biotechnological market potential due to their broad substrate specificity in diverse fields of industrial applications, such as in pulp delignification (Sathishkumar et al 2012;Minussi et al 2007), textile dye bleaching (Lantto et al 2004;Pazarloglu et al 2005;Rodríguez-Couto 2012), wastewater detoxification (Tavares et al 2009), xenobiotic detoxification (Coelho et al 2010;Torres et al 2003), food industry applications (Alper and Acar 2004;Tannoven and Eksi 2005), biosensor applications (Vianello et al 2004), and green chemistry (Riva 2006).…”