Application of AP-MALDI Imaging Mass Microscope for the Rapid Mapping of Imipramine, Chloroquine, and Their Metabolites in the Kidney and Brain of Wild-Type Mice

Islam, Ariful; Sakamoto, Takumi; Zhai, Qing; Rahman, Md. Muedur; Mamun, Md. Al; Takahashi, Yutaka; Kahyo, Tomoaki; Setou, Mitsutoshi

doi:10.3390/ph15111314

Cited by 14 publications

(16 citation statements)

References 38 publications

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“…Findings from the AP-MALDI-MSI methodology were contrasted with those from the traditional DESI-MSI and MALDI-MSI methods. 158…”

Section: Ambient Liquid Extraction Techniques and Applicationsmentioning

confidence: 99%

“…Evaluating these medications' therapeutically significant characteristics, effectiveness, and possible adverse reactions can be aided by the results of the investigation. 158…”

Section: Ambient Liquid Extraction Techniques and Applicationsmentioning

confidence: 99%

“…But there is currently little proof of their location in many organs. 158 Islam et al (2022) developed AP-MALDI-MSI, also referred to as iMScopeTM QT, in a recent study to quickly map imipramine, chloroquine, and its derivatives in mice that are wild-type. Findings from the AP-MALDI-MSI methodology were contrasted with those from the traditional DESI-MSI and MALDI-MSI methods.…”

Section: Minireviewmentioning

confidence: 99%

“…Findings from the AP-MALDI-MSI methodology were contrasted with those from the traditional DESI-MSI and MALDI-MSI methods. 158 It was discovered that whereas chloroquine and its derivatives were substantially collected in the medulla of the kidneys and pelvis of mice, imipramine and its derivatives were greatly deposited in the cortex of the kidney of mice. 158 Various sections of mice's brains exhibited a larger buildup of imipramine (Fig.…”

Section: Minireviewmentioning

confidence: 99%

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Recent developments and applications of ambient mass spectrometry imaging in pharmaceutical research: an overview

Kumar

2024

Anal. Methods

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“…Findings from the AP-MALDI-MSI methodology were contrasted with those from the traditional DESI-MSI and MALDI-MSI methods. 158…”

Section: Ambient Liquid Extraction Techniques and Applicationsmentioning

confidence: 99%

“…Evaluating these medications' therapeutically significant characteristics, effectiveness, and possible adverse reactions can be aided by the results of the investigation. 158…”

Section: Ambient Liquid Extraction Techniques and Applicationsmentioning

confidence: 99%

Section: Minireviewmentioning

confidence: 99%

Section: Minireviewmentioning

confidence: 99%

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Recent developments and applications of ambient mass spectrometry imaging in pharmaceutical research: an overview

Kumar

2024

Anal. Methods

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“…An atmospheric pressure (AP)-MALDI-MSI instrument (iMScope QT, Shimadzu, Japan) has emerged as a promising tool featuring high-speed data acquisition and high spatial resolution. Our group has demonstrated the ability of iMScope QT in the rapid imaging of small drugs and their metabolites in tissues, outperforming conventional vacuum MALDI-MSI and desorption electrospray ionization-MSI (DESI-MSI) …”

Section: Introductionmentioning

confidence: 99%

Detection of Distinct Distributions of Acetaminophen and Acetaminophen-Cysteine in Kidneys up to 10 μm Resolution and Identification of a Novel Acetaminophen Metabolite Using an AP-MALDI Imaging Mass Microscope

Mamun

Rahman

Sakamoto

et al. 2023

J. Am. Soc. Mass Spectrom.

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Drug distribution studies in tissue are crucial for understanding the pharmacokinetics and potential toxicity of drugs. Recently, matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has gained attention for drug distribution studies due to its high sensitivity, label-free nature, and ability to distinguish between parent drugs, their metabolites, and endogenous molecules. Despite these advantages, achieving high spatial resolution in drug imaging is challenging. Importantly, many drugs and metabolites are rarely detectable by conventional vacuum MALDI-MSI because of their poor ionization efficiency. It has been reported that acetaminophen (APAP) and one of its major metabolites, APAP-Cysteine (APAP-CYS), cannot be detected by vacuum MALDI-MSI without derivatization. In this context, we showed the distribution of both APAP and APAP-CYS in kidneys at high spatial resolution (25 and 10 μm) by employing an atmospheric pressure-MALDI imaging mass microscope without derivatization. APAP was highly accumulated in the renal pelvis 1 h after drug administration, while APAP-CYS exhibited characteristic distributions in the outer medulla and renal pelvis at both 30 min and 1 h after administration. Interestingly, cluster-like distributions of APAP and APAP-CYS were observed in the renal pelvis at 10 μm spatial resolution. Additionally, a novel APAP metabolite, tentatively coined as APAP-butyl sulfate (APAP-BS), was identified in the kidney, brain, and liver by combining MSI and tandem MSI. For the first time, our study revealed differential distributions of APAP, APAP-CYS (in kidneys), and APAP-BS (in kidney, brain, and liver) and is believed to enhance the understanding of the pharmacokinetics and potential nephrotoxicity of this drug.

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Spatial neurolipidomics—MALDI mass spectrometry imaging of lipids in brain pathologies

Jha,

Blennow,

Zetterberg

et al. 2024

J Mass Spectrom

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Given the complexity of nervous tissues, understanding neurochemical pathophysiology puts high demands on bioanalytical techniques with respect to specificity and sensitivity. Mass spectrometry imaging (MSI) has evolved to become an important, biochemical imaging technology for spatial biology in biological and translational research. The technique facilitates comprehensive, sensitive elucidation of the spatial distribution patterns of drugs, lipids, peptides, and small proteins in situ. Matrix‐assisted laser desorption ionization (MALDI)‐based MSI is the dominating modality due to its broad applicability and fair compromise of selectivity, sensitivity price, throughput, and ease of use. This is particularly relevant for the analysis of spatial lipid patterns, where no other comparable spatial profiling tools are available. Understanding spatial lipid biology in nervous tissue is therefore a key and emerging application area of MSI research. The aim of this review is to give a concise guide through the MSI workflow for lipid imaging in central nervous system (CNS) tissues and essential parameters to consider while developing and optimizing MSI assays. Further, this review provides a broad overview of key developments and applications of MALDI MSI‐based spatial neurolipidomics to map lipid dynamics in neuronal structures, ultimately contributing to a better understanding of neurodegenerative disease pathology.

show abstract

Application of AP-MALDI Imaging Mass Microscope for the Rapid Mapping of Imipramine, Chloroquine, and Their Metabolites in the Kidney and Brain of Wild-Type Mice

Cited by 14 publications

References 38 publications

Recent developments and applications of ambient mass spectrometry imaging in pharmaceutical research: an overview

Recent developments and applications of ambient mass spectrometry imaging in pharmaceutical research: an overview

Detection of Distinct Distributions of Acetaminophen and Acetaminophen-Cysteine in Kidneys up to 10 μm Resolution and Identification of a Novel Acetaminophen Metabolite Using an AP-MALDI Imaging Mass Microscope

Spatial neurolipidomics—MALDI mass spectrometry imaging of lipids in brain pathologies

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