Application of an in-line imprinted polymer column in a potentiometric flow-injection chemical sensor to the determination of the carbamate pesticide carbaryl in complex biological matrices

Abstract

A flow-injection biosensor-like system based on a nonenzymatic approach has been developed to determine the carbamate pesticide carbaryl in complex biological samples without lengthy and expensive extraction steps. Molecularly imprinted polymeric beads were used to immobilize carbaryl from biological samples. pH variation permitted the elution of carbaryl from the binding cavity to the flow cell. A pH electrode was used to detect changes in the charge of carbaryl in the sample solution resulting from the protonation and deprotonation of the molecule over different pH ranges. At pH 2.0, the secondary amine group is protonated, giving a (+1) charge to the carbaryl molecule. At pH 8.0, the ionized carbaryl loses a proton to become neutral, changing the local pH of the flow cell. The pH change at the flow cell generated by the deprotonation of carbaryl ion in alkaline medium was used to determine the carbaryl concentration. Parameters influencing the performance of the system were optimized for use in the detection procedure. The validated biosensor-like system had a carbaryl detection limit of 10.0 microg/mL and a response that was linear (r2>0.98) over the concentration range of 10.0-00 microg/mL.

Current trends in solid-phase microextraction (SPME) fibre coatings

“…95 But they also have a couple of drawbacks: they are highly selective towards only certain groups of compounds, 96 and, even though they work efficiently in an aqueous medium, the fact that the polymer chains regroup in some organic media leads to internal deformations of the polymer structure. 37 MIPs are synthesised mainly for analytical purposes, where they are used in SPME for sampling pesticides, 97,98 triazines, 99,100 phenols, heavy metals, antibiotics, 101,102 as well as many other compounds from samples with a complex matrix composition like blood, 103,104 urine, 105 food 106,107 and environmental samples. 86 MIPs are also used in catalysis, 108 in measurement biosensors 109 -mainly the chemical sensors that enable an analyte to be determined directly in the sample matrix, 110 and as fillers in syringe-like arrangements used in the analysis of blood samples.…”

Determination of phenformin hydrochloride using molecular imprinting technology coupled with flow‐injection chemiluminescence

“…The advantages of MIPs include their stability in a wide range of environments, their facility for sensor microfabrication, and their ability to detect analytes that are difficult or impossible to sense by immunoassay . MIPs have been utilized in solid‐phase extraction , artificial antibodies , sensors and chromatographic media for their potential absorptive selectivity to the target molecules and the related compounds.…”

The Effect on Adsorption Performance of Molecularly Imprinted Polymer Using the Different Functional Monomers

“…So the MIPs show higher selectivities and affinities in rebinding the template and its analogues. MIPs have been used in areas such as sensors [1], chiral separations [2], and analysis of environmental samples [3]. Recently, the use of MIPs as separation materials for extracting certain active components directly from herbs has been reported [4~6].…”