Application of an immunoaffinity column sample clean-up to the determination of aflatoxin M1 in cheese

Aflatoxins in Organic Milk and Dairy Products

“…To determine the presence of aflatoxin M 1 in the organic milk and cheese samples, a Ridascreen Aflatoxin M 1 elisa kit (Ribopharm) was used. A parallel analysis of the organic milk and cheese samples was performed using HPLC, according to the method described by Sharman et al (1989). The samples of cheese from commercial sources were analysed using only the latter method.…”

Enzyme-assisted extraction for the HPLC determination of aflatoxin M1 in cheese

“…In the clean-up step IA columns are widely used and have replaced adsorption or partition chromatography on pre-packed or prepared columns. Generally, the toxin is extracted with chloroform or dichloromethane (Bijl, Van Peteghem, & Dekeyser, 1987;Cavaliere et al, 2006;Colak, Hampikyan, Ulusoy, & Ergun, 2006;Dragacci, Gleizes, Fremy, & Candlish, 1995;Elgerbi, Aidoo, Candlish, & Tester, 2004;Piva, Pietri, Galazzi, & Curto, 1988;Sharman, Patey, & Gilbert, 1989).…”

“…The mixture is transferred to a separating funnel and the lower layer is collected and purified through an IA column (Sharman et al, 1989). This method has been widely used and has been adopted as an official AOAC method (AOAC, 2005a).…”

Testing the suitability of different high-performance liquid chromatographic methods to determine aflatoxin M1 in a soft fresh Italian cheese

“…In the case of complex food matrices, methods must be developed and validated for toxin-matrix combinations, and in the case of cheese, few methods have been investigated [27][28][29][30][31][32][33][34][35].…”