Application of an automated particle‐based immunosensor for the detection of aflatoxin B<sub>1</sub>in foods

Strachan, Norval James Colin; John, Preethy; Millar, I.G.

doi:10.1080/09540109709354948

Cited by 18 publications

(7 citation statements)

References 12 publications

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“…One study used monoclonal IgG antibodies, and could achieve a detection range of 0.2–10 ng/g with prior concentration of AFs using IACs [ 5 ], and the other used multimeric recombinant antibody fragments, and had 0.2–24 ng/mL detection range [ 31 ]. Other optical AF sensors utilized either fluorescent [ 25 , 41 ] or chemiluminescent [ 27 ] labels for limits of detection ranging from 0.1 to 1.5 ng/mL. Therefore, the present study was the first development of competitive AF immunosensor development without using any kind of labels or tags, which was possible with the use of an IgA isotype antibody.…”

Section: Resultsmentioning

confidence: 99%

Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element

Ertekin

Öztürk

2016

Sensors

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This study introduces the use of an IgA isotype aflatoxin (AF) specific monoclonal antibody for the development of a highly sensitive Quartz Crystal Microbalance (QCM) immunobiosensor for the detection of AF in inhibitory immunoassay format. The higher molecular weight of IgA antibodies proved an advantage over commonly used IgG antibodies in label free immunobiosensor measurements. IgA and IgG antibodies with similar affinity for AF were used in the comparative studies. Sensor surface was prepared by covalent immobilization of AFB1, using self assembled monolayer (SAM) formed on gold coated Quartz Crystal, with 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxy succinimide (EDC/NHS) method using a diamine linker. Nonspecific binding to the surface was decreased by minimizing the duration of EDC/NHS activation. Sensor surface was chemically blocked after AF immobilization without any need for protein blocking. This protein free sensor chip endured harsh solutions with strong ionic detergent at high pH, which is required for the regeneration of the high affinity antibody-antigen interaction. According to the obtained results, the detection range with IgA antibodies was higher than IgG antibodies in QCM immunosensor developed for AFB1.

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Section: Resultsmentioning

confidence: 99%

Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element

Ertekin

Öztürk

2016

Sensors

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“…Another study [13] found an average recovery of 91% for pre-packed florisil column clean up. Studies using immunoaffinity columns have reported recoveries of the range 71-92% total aflatoxin, 82-109% for AFB 1 and 92-101% for AFB 1 [15,16].…”

Section: Discussionmentioning

confidence: 99%

“…These include aluminium oxide-packed minicolumns [10], phenyl-packed minicolumns [11], and Sep-Pak Florisil cartridges [12,13,14]. Other studies [15,16] have reported the use of immunoaffinity columns for clean up in RP-HPLC using post-column derivatisation with bromine. A major disadvantage of pre-packed columns is that they are expensive and must be disposed of after single clean up procedures.…”

Section: Introductionmentioning

confidence: 99%

Adaptation of glass columns for clean-up RP-HPLC determination of aflatoxins with post-column derivatisation with bromine and fluorescence detection.

Owino¹

2009

African Journal of Food, Agriculture, Nutrition and Development

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Consumption of aflatoxin-contaminated foods in developing countries may not only cause cancer in the general population, but is also associated with stunting and immune suppression in children. Accurate determination of aflatoxins in foods is important for the prevention of the above health risks. This study aimed to evaluate the possibility of using cheap florisil-packed glass columns instead of relatively complex and expensive pre-packed cartridges for afltoxin clean-up prior to HPLC analysis. Additionally, effects of aflatoxin extract storage temperature, and the moisture content of florisil on aflatoxin recovery were assessed. Results showed that the standard curve was highly linear as depicted by the high correlation coefficient for all four aflatoxins [0.99, 0.99, 0.97 and 0.98, respectively for AFG 1 , AFB 2 and AFB 1 and AFG2. The retention times over a total run time of 20 minutes were 14.8 (SD 0.4), 12.4 (SD 0.1), 10.7 (SD 0.3) and 9.1 (SD 0.3) minutes, respectively for AFB 1 , AFB 2 , AFG 1 and AFG 2 . Florisil powder that had been dried for 12 hours at 100 o C, then hydrated with water at 5% (w/v) before packing on glass columns gave slightly higher recovery for all aflatoxins (100.3% AFB 1 , 93.1% AFB 2 , 83.8% AFG 1 and 71.1% AFG 2 ) compared to the pre-packed cartridges (96.4% AFB 1 , 91.5% AFB 2 , 80.6% AFG 1 and 66.9% AFG 2 ). There was 10% and 20% aflatoxin loss at 4 C, the loss range was 20-50% and 30-55%, respectively. Aflatoxin recoveries ranged from 120-160%, 90-110%, 80-98%, and 55-74% for florisil which had been hydrated with water at 2.5%, 5% and 10%, respectively. Glass columns are cheap and can be used many times and are a feasible and sustainable alternative to pre-packed florisil cartridges for aflatoxin clean up to ensure food safety, especially in resource poor settings.

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“…That instrument was used by Strachan et al (1997) to develop an application for detection of aflatoxin B 1 in nut puree, peanut butter, and pistachio meal. The aflatoxin microbead sensor was able to detect 1 mL of a 1.44-ng/mL solution of AFB 2 .…”

Section: Maragosmentioning

confidence: 99%

Emerging Technologies for Mycotoxin Detection

Maragos

2004

Journal of Toxicology: Toxin Reviews

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Application of an automated particle‐based immunosensor for the detection of aflatoxin B₁in foods

Cited by 18 publications

References 12 publications

Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element

Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element

Adaptation of glass columns for clean-up RP-HPLC determination of aflatoxins with post-column derivatisation with bromine and fluorescence detection.

Emerging Technologies for Mycotoxin Detection

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Application of an automated particle‐based immunosensor for the detection of aflatoxin B1in foods

Cited by 18 publications

References 12 publications

Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element

Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element

Adaptation of glass columns for clean-up RP-HPLC determination of aflatoxins with post-column derivatisation with bromine and fluorescence detection.

Emerging Technologies for Mycotoxin Detection

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Application of an automated particle‐based immunosensor for the detection of aflatoxin B₁in foods