Application of β-cyclodextrin/MnFe2O4 magnetic nanoparticles as a catalyst for fast chemiluminescence determination of glutathione in human blood using luminol-diperiodatoargentate(III) System

Abstract

Este trabalho baseia-se na quimioluminescência (CL) intensificada da reação entre luminol e diperiodato de prata (III) (K[Ag(H 3 IO 6 ) 2 ], DPA) em solução alcalina para determinação da glutadiona (GSH). Na presença de nanopartículas magnéticas do complexo de inclusão β-ciclodextrina (β-CD)/nanopartículas magnéticas MnFe 2 O 4 (MNPs), os resultados experimentais mostraram que a emissão CL foi muito rápida (tempo de resposta de ca. 1 s, parâmetro muito importante em análise clínica) e aguda (sinal CL aumentado de 9 vezes). Os efeitos catalíticos das MNPs β-CD/MNPs MnFe 2 O 4 na resposta CL do sistema luminol-DPA foram investigados. A linearidade do método dependeu da concentração da GSH nos intervalos de 5,0 × 10 -8 a 4,0 × 10 -6 mol L -1, o limite de detecção calculado foi 1,5 × 10 -8 mol L -1 e desvio padrão relativo de 2,8% para 9 replicatas de GSH 8,0 × 10 -7 mol L -1 . O método proposto foi aplicado com sucesso na determinação rápida e sensível da GSH em amostras de sangue humano.This work is based on the enhanced chemiluminescence (CL) of the reaction between luminol and diperiodatoargentate(III) (DPA) {K[Ag(H 3 IO 6 ) 2 ]} in alkaline solution for determination of glutathione (GSH). In the presence of inclusion complex β-cyclodextrin (β-CD)/MnFe 2 O 4 magnetic nanoparticles (MNPs), the experimental results showed that the CL emission was very fast (response time of about 1 s, which is a very important parameter in the clinical analysis) and sharp (CL signal increased 9 times). The catalytic effects of β-CD/MnFe 2 O 4 MNPs on the CL response of luminol-DPA system were investigated. The linearly of method depended on GSH concentration in the ranges of 5.0 × 10 -8 to 4.0 × 10 -6 mol L -1 and limit of detection calculated was 1.5 × 10 -8 mol L -1 and relative standard deviation of 2.8% for 9 replicated measurements of 8.0 × 10 -7 mol L -1 GSH. The proposed method was successfully applied for rapid and sensitive determination of GSH in human blood samples.

Biomedical Probes Based on Inorganic Nanoparticles for Electrochemical and Optical Spectroscopy Applications

“…Moreover, they are easily oxidized and dissolved in acid media, leading to poor stability [ 5 ]. Their plethora of applications benefit from their low toxicity, biocompatibility, and easy separation utilizing external magnetic fields [ 7 , 8 , 9 , 10 ]. A facile separation of magnetic nanoparticles is not only able to reduce the background interference but also enhance target immobilization [ 11 ].…”

A chemiluminescence method to detect hydroquinone with water‐soluble sulphonato‐(salen)manganese(III) complex as catalyst

“…With the continuing research into the chemiluminescence system, many chemiluminescence reactions have been discovered, especially for luminol. In recent years, many CL systems for luminol with several reagents, such as hydrogen peroxide , potassium ferricyanide , potassium permanganate , potassium periodate , silver nitrate , carboxymethyl chitosan , the Cu(III) complex , the Ag(III) complex , hypochlorite , albumin and colloidal MnO 2 have been reported. Among these, the hydrogen peroxide–luminol CL system is simple and stable, and therefore widely applied.…”