Application of accelerated storage test to lyophilized plant viruses

Yordanova, A.; Stoimenova, E.; Donev, Todor

doi:10.1007/bf00180406

Cited by 5 publications

(4 citation statements)

References 5 publications

(1 reference statement)

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“…Therefore, it is desirable to know for how long the cells will remain viable and how viability will be influenced by the different preservation methods. Because of the difficulty in testing the effects of long-term storage on microbial viability, storage based on accelerated conditions that mimic the effect of long-term preservation has been previously used to promote the process of cell ageing [29,30]. It has been shown that accelerated storage of liquid-dried bacterial cultures for 2 weeks at 37 °C can simulate 20 years of storage at 5 °C [29].…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that accelerated storage of liquid-dried bacterial cultures for 2 weeks at 37 °C can simulate 20 years of storage at 5 °C [29]. The process of accelerated storage has shown to be a useful tool to evaluate the efficiency of different preservation methods as well as to predict the behaviour of preserved microbiological cells after long-term storage [29,30].…”

Section: Introductionmentioning

confidence: 99%

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Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains

et al. 2019

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This study aims to evaluate the effect of freeze-drying and long-term storage on the biotechnological potential of Aspergillus section Nigri strains. Twelve selected strains were freeze-dried and aged by accelerated storage, at 37 °C in the dark, for 2 and 4 weeks. To assess possible changes as a consequence of the ageing in the freeze-drying ampoules, morphological characteristics, mycotoxins and enzymes production, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALTI-TOF MS) spectra, and M13 phage probe fingerprinting were used as part of a polyphasic approach. Phenotypical changes were observed; nevertheless, they did not substantially affect the potential biotechnological use of these strains. The activity of hydrolytic enzymes (protease, carboxymethylcellulase, xylanase, pectinase and mannanase) was maintained or increased after freeze-drying. MALDI-TOF MS data originated spectra that grouped, for the majority of samples, according to strain independently of preservation time point. M13 profiles revealed the presence of some genetic polymorphisms after preservation. However, the three studied times still clustered for more than 50% of strains. Our results show that the studied strains maintain their biotechnological potential after preservation, with minimal phenotypic alterations. These findings provide evidence that freeze-drying preservation is a suitable option to preserve biotechnologically relevant aspergilli strains from section Nigri, and one should consider that the observed effects might be species/strain-dependent.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains

et al. 2019

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“…The purified preparations with a concentration of 1 mg/ml were freeze-dried in ampoules in aliquots of 0.2 ml. Protective medium for the variants of sap and purified preparation was the combination of 5 % sorbitol and 3.6 % dextran 40 (8). The parameters of the freeze-drying were according to Donev et al (2).…”

Section: Methodsmentioning

confidence: 99%

“…They have survived in dried leaves and lyophilized sap for decades (3,6). In several our previous works the preservation of different tobamovirus strains freeze-dried in the form of leaf material, plant sap and purified preparations was investigated by means of an accelerated storage test at elevated temperatures (7,8,9,10). Strain specific differences were observed and the most stable strain survived more than 5 times longer than the most labile one.…”

Section: Introductionmentioning

confidence: 97%

Prediction of the Preservation of Lyophilized Tobamoviruses

Yordanova

Donev

Stoimenova

2005

Biotechnology & Biotechnological Equipment

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Eleven tobamovirus strains were lyophilized in the form of leaves, plant sap with and without protectant, as well as protected purified preparation. Accelerated storage test at 28-81 ºC for 30 to 240 days was applied to the freeze-dried samples. The infectivity was tested on а local host. The survival decrease was represented as pseudo-first order reaction and predictions for stability of lyophilizates at real storage conditions (4 ºC) were developed by means of Arrhenius equation. The preservation depended on the lyophilic form and was strain specific. Some strains were expected to loose 90% of the survival after 1-3 years while others -after more than 25 years in unprotected variants. The preservation as protected purified preparations will be more than 60-70 years. Among the tested strains of tomato mosaic virus the highest longevity for the strains of group "2" after Pelham was predicted. The accelerated storage test and the prediction facilitate the maintenance of a plant virus collection.

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Assay, Detection, and Diagnosis of Plant Viruses

Hull

2014

Plant Virology

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Application of accelerated storage test to lyophilized plant viruses

Cited by 5 publications

References 5 publications

Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains

Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains

Prediction of the Preservation of Lyophilized Tobamoviruses

Assay, Detection, and Diagnosis of Plant Viruses

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