Assay, Detection, and Diagnosis of Plant Viruses

Hull, Roger

doi:10.1016/b978-0-12-384871-0.00013-3

Cited by 9 publications

(5 citation statements)

References 304 publications

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“…Dot-blot hybridization is a nucleic acid-based detection technique for high throughput or large-scale sample testing. It relies on the direct and semi-quantitative detection of a specific nucleotide sequence dotted on a membrane without prior electrophoresis-based separation and gel-to-membrane transference of the target sequence [ 54 ]. Technically, the target DNA or RNA sample extracted from the organism or cell in a specimen is spotted onto a membrane before hybridization by a labeled nucleic acid probe.…”

Section: Resultsmentioning

confidence: 99%

Nucleic Acid-Based Detection of Pythium insidiosum: A Systematic Review

Sridapan

Krajaejun

2022

JoF

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Pythiosis, a life-threatening infectious condition caused by Pythium insidiosum, has been increasingly reported in humans and animals worldwide. Antifungal drugs usually fail to control the pathogen. The surgical removal of an infected organ is the treatment of choice. Many affected patients die due to advanced infection. A timely and accurate diagnosis could lead to a better prognosis in pythiosis patients and save their lives. Although a standard culture method is available in microbiological laboratories, it is time-consuming, laborious, and insensitive for P. insidiosum identification. Immunological assays have been developed to improve the diagnosis of pythiosis. However, immunological methods are commercially unavailable and primarily detect anti-P. insidiosum antibodies, which constitute indirect evidence of pythiosis, making it challenging to differentiate a past from a recent infection. Moreover, such immunological tests cannot diagnose patients with a local infection, such as in the eye. Nucleic acid-based tests (NATs) are efficient for the direct and rapid detection of P. insidiosum DNA in trace-amount or culture-negative specimens. The reagents and equipment required for NATs are usually available in molecular diagnostic laboratories. Herein, we provide a systematic review to comprehensively present the principal and clinical usages, advantages, and limitations of such NATs in the detection of P. insidiosum. Various NATs have been established to detect P. insidiosum, which can be classified into amplification-based (i.e., PCR assays, isothermal tests, and next-generation sequencing methods) and non-amplification-based (i.e., DNA hybridization) techniques. This concise review on NATs constitutes an up-to-date reference with which healthcare professionals can learn about and decide upon which detection method is suitable for their respective laboratory environments.

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Section: Resultsmentioning

confidence: 99%

Nucleic Acid-Based Detection of Pythium insidiosum: A Systematic Review

Sridapan

Krajaejun

2022

JoF

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“…The density gradient can be prepared, typically, with sucrose, glycerol, or another aqueous solution, and it is created inside of a centrifuge tube [1]. The solution that fills the centrifuge tube originates a decreasing density gradient from the bottom to the top of the tube [39].…”

Section: Density Gradient Centrifugationmentioning

confidence: 99%

“…In the isopycnic centrifugation, the process continues until most of the particles reach their isopycnic position in the centrifuge tube with the density gradient, that is, a position where their density equals the density of the medium. This type of centrifugation separates different particles based only on their different densities [39]. This way, one disadvantage of the isopycnic method is its incompatibility with metallic NPs separation, since these are denser than the highest densities attainable in aqueous media gradients (<1.7 g/cm 3 ), making it not possible to separate metallic NPs with the isopycnic centrifugation method.…”

Section: Density Gradient Centrifugationmentioning

confidence: 99%

From Impure to Purified Silver Nanoparticles: Advances and Timeline in Separation Methods

2021

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AgNPs have exceptional characteristics that depend on their size and shape. Over the past years, there has been an exponential increase in applications of nanoparticles (NPs), especially the silver ones (AgNPs), in several areas, such as, for example, electronics; environmental, pharmaceutical, and toxicological applications; theragnostics; and medical treatments, among others. This growing use has led to a greater exposure of humans to AgNPs and a higher risk to human health and the environment. This risk becomes more aggravated when the AgNPs are used without purification or separation from the synthesis medium, in which the hazardous synthesis precursors remain unseparated from the NPs and constitute a severe risk for unnecessary environmental contamination. This review examines the situation of the available separation methods of AgNPs from crude suspensions or real samples. Different separation techniques are reviewed, and relevant data are discussed, with a focus on the sustainability and efficiency of AgNPs separation methods.

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“…The transmissions of viruses from plant to plant by vectors provide the main method of spread in the field for many viruses including MCMV that cause severe economic loss (Hull, 2014). The studies on the MLN (MCMV is the main component) distribution and factors associated with its epidemic show that the spread of MLN causing viruses (MCMV and SCMV) are linked to the free movement of insect vector and continuous availability of the host plants (Regassa et al, 2020).…”

Section: Insect Vectorsmentioning

confidence: 99%

Occurrence, Distribution, Economic Importance and Management of Maize Chlorotic Mottle Virus: A Review

Regassa¹

2021

ALST

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Assay, Detection, and Diagnosis of Plant Viruses

Cited by 9 publications

References 304 publications

Nucleic Acid-Based Detection of Pythium insidiosum: A Systematic Review

Nucleic Acid-Based Detection of Pythium insidiosum: A Systematic Review

From Impure to Purified Silver Nanoparticles: Advances and Timeline in Separation Methods

Occurrence, Distribution, Economic Importance and Management of Maize Chlorotic Mottle Virus: A Review

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