Application of a streamlined LC-MS/MS methodology for the determination of atropine and scopolamine in cereals from Asian and African countries

Occurrence and Chemistry of Tropane Alkaloids in Foods, with a Focus on Sample Analysis Methods: A Review on Recent Trends and Technological Advances

“…However, in a more recent study, Marín-Sáez et al [18] analyzed 18 cereal-based baby foods finding a positive sample of biscuits with 11.5 µg/kg of At and 2.8 µg/kg of Sc, which contained TAs levels above the EU regulatory limit of 1 µg/kg for each alkaloid. On the other hand, in a study of Baslé et al [19] with samples from Asian and African countries, At and Sc were not detected in any of the four infant-cereals products analyzed, which were purchased in Singapore.…”

“…It was firstly reported in 1973 to separate At, Sc, homatropine and apoatropine [37]. Usually, TAs are analyzed in reverse phase mode with conventional C18 stationary phases [13,14,[17][18][19][21][22][23]25,[27][28][29][30][38][39][40], although C8 [5] and HILIC columns have also been used [16,26,32,33,41]. The mobile phase commonly consisted of a mixture of organic phases (methanol, acetonitrile or mixtures of them, sometimes acidified) and aqueous phases (water with different additives such as acetic and formic acids and salts such as ammonium hydroxide, ammonium formate or ammonium acetate).…”

“…Regarding detectors, MS detectors predominate, considering that the lack of a strong chromophore in TAs molecules requires detections at low wavelengths, making it difficult to identify these compounds with UV detectors. For this reason, different analyzers based on MS are used to determine TAs, usually triple quadrupole (QqQ) [13,14,[17][18][19][21][22][23]25,[28][29][30]33,41,42] but also quadrupole-ion trap (QTRAP) [27,38]. High-resolution mass spectrometry analyzers (HRMS) such as the Orbitrap analyzer [5,16,18,26,32,39,40] or its variant quadrupoleorbitrap (Q-Orbitrap) [5] have also been used.…”

Simultaneous determination of acepromazine and atropine residues in porks, livers, kidneys by Ultra‐High‐Performance Liquid Chromatography‐Tandem Mass Spectrometry

“…The sample treatment procedures were referenced from Q. Baslé et al and have been modified to suit the matrix sample. [27] Briefly, first, a representative sample (50 g) was chopped into small slices and finely ground with a blender to homogenize it before extraction. 10.0±0.1 g of the sample was transferred into a 50-mL centrifuge tube.…”

Levels of Alternaria Toxins in Selected Food Commodities Including Green Coffee

“…Best results were obtained using Na2SO4/NaCl (4 g + 1 g) salts to induce liquid-liquid partition and hexane to remove co-extracted fat as a subsequent clean-up step. This clean-up procedure is extensively used for mycotoxins and tropane alkaloids analyses in Nestlé laboratories [26,28,32]. To reduce the strong matrix effects observed for ALT and AOH in some Historically, most methods for monitoring Alternaria toxins were devoted to the analysis of high-water content commodities such as tomatoes and tomato-based products and fruit juices [24,25,30,31].…”