Application of a solid-phase fluorescence immunoassay to determine amoxicillin residues in fish tissue

Jung, Won Chul; Lee, Hu-Jang

doi:10.1556/avet.58.2010.1.9

Cited by 11 publications

(8 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, Carballeira et al (2012) studied the toxicity of AM on fish and found similar sensitivities to all substances tested. In addition, Park et al (2010) detected AM in fish tissue after the 3 rd day of spiking in water. Liu et al (2012) studied the toxicity AM and microcystins against algae and found that Spiramycin is more toxic to M. aeruginosa than AM according to their EC 50 values.…”

Section: Introductionmentioning

confidence: 96%

Toxicity of Amoxicillin and Erythromycin to Fish and Mosquito

El‐Nahhal

EL-dahdouh²

2015

Ecotoxicol. Environ. Contam.

View full text Add to dashboard Cite

This study characterized the toxicity of Amoxicillin (AM), Erythromycin (ER) and Endosulfan (EN) to fish and mosquito larvae obtained from a certified fish farm and from Wadi Gaza. The acute toxicity tests were determined by calculating mortality percentage of fish and mosquitoes through a gradient concentration of the tested compounds. Mortality percentage, exposure time, LC 50 and LT 50 were taken as indicators of toxicity. The results showed that AM was the potent compound against fish with LC 50 value lower that EN. Toxicity to fish is in the following order: AM > EN > ER whereas the toxicity to mosquitoes is in the following order: ER > EN > AM. Fish are more sensitive to AM than mosquitoes whereas mosquitoes are more sensitive to ER than fish. The interesting outcome of the study is the calculated LC 50 values are far below the concentration found in different water systems. Mixture toxicity of the tested antibiotics indicates antagonistic effect on both fish and mosquitoes.

show abstract

Section: Introductionmentioning

confidence: 96%

Toxicity of Amoxicillin and Erythromycin to Fish and Mosquito

El‐Nahhal

EL-dahdouh²

2015

Ecotoxicol. Environ. Contam.

View full text Add to dashboard Cite

show abstract

“…Thus, most countries have turned to cultured Clarias species for their high reproductive capacity under natural and controlled conditions, high growth rate, tolerance of bad water conditions and high nutritional value (Ikpi & Offem, ; Khan & Abidi, ; Maneechot et al, ). Several trials using herbal and alternative medicine in fish culture (Hoseinifar et al, ) have been performed to overcome the side effects of using antibiotics for controlling different diseases that cause severe issues in humans, especially drug resistance and antibiotic residues (Chafer‐Pericas et al, ; Obaidat, Salman, & Lafi, ; Park, Jung, & Lee, ). Probiotic (Mehdinejad, Imanpour, & Jafari, ; Meidong et al, ), prebiotic (Bai, Tsuruta, & Nishino, ; Forsatkar, Nematollahi, Rafiee, Farahmand, & Lawrence, ) and even immune‐modulating agents have been used (Fuchs, Schmidt, Slater, Buck, & Steinhagen, ; Valenzuela, Rodriguez, Modak, & Imarai, ).…”

Section: Discussionmentioning

confidence: 99%

Dietary supplementation with low molecular weight sodium alginate improves growth, haematology, immune reactions and resistance againstAeromonas hydrophilainClarias gariepinus

2019

View full text Add to dashboard Cite

A few studies have illustrated the effects of sodium salt derived from alginic acid on different fish species. However, little is known about the effect of sodium alginate on catfish (Clarias gariepinus). Therefore, this study was performed to assess the use of low molecular weight sodium alginate (LMWSA) in C. gariepinus. A total of 180 apparently healthy C. gariepinus with a mean body weight of 45 g were randomly divided into three equal groups (D1, D2 and D3). D1 the control group received a control diet, while D2 and D3 received 1% and 3% LMWSA, respectively, for 8 weeks. A challenge test against Aeromonas hydrophila was performed on 15 randomly selected catfish for 15 days. At the end of the experiment, catfish in D3 that received a diet of 3% LMWSA showed significant increases in the final body weight, weight gain and thermal‐unit growth coefficient compared with those in D2 and D1. There was a significant decrease in the erythrogram in D1 after the 4‐day pathogen challenge. A leucogram revealed leucocytosis, heterophilia and lymphocytosis in catfish in D2 and D3 compared with those in D1. After the 4‐day challenge, the following changes took place: lysozyme, nitric oxide, phagocytic activity and the respiratory burst were significantly elevated in catfish that received LMWSA and were more pronounced in D3 than in D1. The mortalities of catfish have been stopped after pathogen challenge from 8‐day in D1 and D2 where at 6‐day in D3. Thus, administration of 1% and 3% LMWSA enhances the growth, immune response and resistance of C. gariepinus against A. hydrophila.

show abstract

“…In parallel to these two approaches, rapid tests specific to β-lactam antibiotics, for example, Charm test and Parallux, are often adopted for fast screening. 17,26−28 Furthermore, there has been a growing trend in developing innovative biosensors for simple, speedy, reliable, and efficient screening of antibiotic residues in food and clinical specimens. 19,29−32…”

Section: Introductionmentioning

confidence: 99%

Thermostable β-Lactamase Mutant with Its Active Site Conjugated with Fluorescein for Efficient β-Lactam Antibiotic Detection

Au¹,

Tsang²,

So³

et al. 2019

ACS Omega

View full text Add to dashboard Cite

Monitoring the β-lactam antibiotic level has been an important task in food industry and clinical practice. Here, we report the development of a fluorescent PenP β-lactamase, PenP-E166Cf/N170Q, for efficient β-lactam antibiotic detection. It was constructed by covalently attaching fluorescein onto the active-site entrance of a thermostable E166Cf/N170Q mutant of a Bacillus licheniformis PenP β-lactamase. It gave a fluorescence turn-on signal toward various β-lactam antibiotics, where the fluorescence enhancement was attributed to the acyl–enzyme complex formed between PenP-E166Cf/N170Q and the β-lactam antibiotic. It demonstrated enhanced signal stability over its parental PenP-E166Cf because of the suppressed hydrolytic activity by the N170Q mutation. Compared with our previously constructed PenPC-E166Cf biosensor, PenP-E166Cf/N170Q was more thermostable and advanced in detecting β-lactams in terms of response time, signal stability, and detection limit. Positive fluorescence signals generated by E166Cf/N170Q in response to the penicillin-containing milk and mouse serum illustrated the feasibility of the biosensor for antibiotic detection in real samples. Taken together, our findings suggest the potential application of PenP-E166Cf/N170Q in biosensing β-lactam antibiotics.

show abstract

Application of a solid-phase fluorescence immunoassay to determine amoxicillin residues in fish tissue

Cited by 11 publications

References 14 publications

Toxicity of Amoxicillin and Erythromycin to Fish and Mosquito

Toxicity of Amoxicillin and Erythromycin to Fish and Mosquito

Dietary supplementation with low molecular weight sodium alginate improves growth, haematology, immune reactions and resistance againstAeromonas hydrophilainClarias gariepinus

Thermostable β-Lactamase Mutant with Its Active Site Conjugated with Fluorescein for Efficient β-Lactam Antibiotic Detection

Contact Info

Product

Resources

About