Application of a novel radioimmunoassay for serotonin in the detection of aromatic l-amino acid decarboxylase activity in isolated renal cortical cells

Waugh, C. J.; Gow, Iain F; Edwards, C. R. W.; Williams, Brent C.

doi:10.1042/bst0170163

Cited by 8 publications

(3 citation statements)

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“…Most of these methods use spectrophotometry , high‐performance liquid chromatography (HPLC) , gas chromatography , chemiluminescence , amperometric and voltammetric principles . A few methods for the estimation of LD and CD have also been reported using potentiometry , radioimmunoassay and flow injection analysis .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous estimation of levodopa and carbidopa by RP‐HPLC using a fluorescence detector: its application to a pharmaceutical dosage form

Raut

Charde

2014

Luminescence

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A reverse-phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the simultaneous estimation of levodopa and carbidopa in bulk and pharmaceutical formulations. Chromatographic separation was achieved by using a C18 reverse-phase column and a mixture of an aqueous phase (10 mM potassium dihydrogen phosphate buffer, pH4.0) and methanol (90:10 v/v) as the mobile phase. Quantitative analysis of levodopa and carbidopa was performed using a fluorescence detector at an excitation wavelength of 280 nm and an emission wavelength of 310 nm. The method was linear between 5 and 500 ng/mL for both levodopa and carbidopa. The detection limits for levodopa and carbidopa were 0.30 and 0.60 ng/mL, respectively, whereas the quantitation limit was 0.80 ng/mL for levodopa and 1.2 ng/mL for carbidopa. The method demonstrated good and consistent recoveries (99.63-100.80% for levodopa and 98.97-100.94% for carbidopa) with low interday and intraday relative standard deviation. The validated method was successfully applied to quantify levodopa and carbidopa simultaneously in a pharmaceutical formulation. The method was found to be precise, sensitive and accurate for the simultaneous determination levodopa and carbidopa in bulk and pharmaceutical formulations.

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Section: Introductionmentioning

confidence: 99%

Simultaneous estimation of levodopa and carbidopa by RP‐HPLC using a fluorescence detector: its application to a pharmaceutical dosage form

Raut

Charde

2014

Luminescence

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show abstract

“…Various methods like spectrophotometry [3][4][5], gas chromatography (GC) [6], high performance liquid chromatography (HPLC) [7], chemiluminescence [8,9], amperometric and voltammetric determination [10][11][12], potentiometry [13], radio-immunoassay [14] and flow injection analysis (FIA) [15][16][17] have been described in literature for the determination of levodopa or carbidopa in various biological samples and pharmaceutical preparations.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous kinetic determination of levodopa and carbidopa by H-point standard addition method

Safavi

Tohidi

2007

Journal of Pharmaceutical and Biomedical Analysis

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“…voltammetric determination, 10 potentiometry, 11 radioimmunoassay 12 and flow injection analysis (FIA). 13 Their simultaneous determination has been traditionally achieved using high-performance liquid chromatography (HPLC).…”

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confidence: 99%

Simultaneous Kinetic-Spectrofluorometric Determination of Levodopa and Carbidopa Using Partial Least-Squares Regression

2009

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Application of a novel radioimmunoassay for serotonin in the detection of aromatic l-amino acid decarboxylase activity in isolated renal cortical cells

Cited by 8 publications

References 0 publications

Simultaneous estimation of levodopa and carbidopa by RP‐HPLC using a fluorescence detector: its application to a pharmaceutical dosage form

Simultaneous estimation of levodopa and carbidopa by RP‐HPLC using a fluorescence detector: its application to a pharmaceutical dosage form

Simultaneous kinetic determination of levodopa and carbidopa by H-point standard addition method

Simultaneous Kinetic-Spectrofluorometric Determination of Levodopa and Carbidopa Using Partial Least-Squares Regression

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