Application of a Multivalent Glycoprobe: Characterization of Sugar-Binding Specificity of Siglec Family Proteins

Yamaji, Taiki; Nakamura, Kyoko; Amari, S.; Suzuki, Akemi; Hashimoto, Yasuhiro

doi:10.1016/s0076-6879(03)01046-2

Cited by 6 publications

(4 citation statements)

References 8 publications

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“…Eleven family members were found to bind sialic acid on glycoconjugates [19]. Although their structures are similar, their binding specificity differs, and they bind specific sialoglycoconjugates [20]. Most Siglec family members are observed on only hematopoietic cells, as they have very low expression in other tissues, but a recent study found that their expression level changed in some tumor cells [21][22][23].…”

Section: Discussionmentioning

confidence: 99%

Integrative Analysis of Siglec-15 mRNA in Human Cancers Based on Data Mining

Li¹,

Huang²,

Chen³

et al. 2020

J. Cancer

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Objective: Cancer is expected to be the leading cause of death worldwide within the 21st century and is the single most important obstacle to extending life expectancy. Unfortunately, the most effective approach to combating cancers remains a complex and unsolved problem. Siglec-15 is a member of the Siglec family and plays a conserved regulatory role in the immune system of vertebrates. Previous studies on Siglec-15 have focused on its function in osteoclast regulation. The purpose of this study was to explore the significance of Siglec-15 mRNA in human cancer mainly based on information obtained from online databases. Method: Data were collected from several online databases. Serial analysis of gene expression (SAGE) and Virtual Northern, UALCAN Database Analysis, Catalog of Somatic Mutations in Cancer (COSMIC) analysis, the cBio cancer genomics portal, Cancer Regulome tools and data, Kaplan-Meier Plotter Analysis and the UCSC Xena website were used to analyze the data. Results: Compared with normal tissues, Siglec-15 up-regulation was widely observed in tuomrs. Differences in Siglec-15 expression were associated with different prognoses. Siglec-15 mutations are widely observed in tumors and interact with different genes in different cancer types. Conclusion: Siglec-15 is a potential target for the expansion of cancer immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Integrative Analysis of Siglec-15 mRNA in Human Cancers Based on Data Mining

Li¹,

Huang²,

Chen³

et al. 2020

J. Cancer

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“…To characterize the glycan-binding specificity of Siglec-7, a high-throughput sensitive assay was developed (Yamaji, Nakamura et al 2003). For the assay we prepared a streptavidinbased neoglycoprotein as a glycoprobe (Hashimoto, Suzuki et al 1998).…”

Section: Binding Specificity Of Siglec-7 Expressed On Cho Cellsmentioning

confidence: 99%

3D Modeling of a Natural Killer Receptor, Siglec-7: Critical Amino Acids for Glycan-Binding and Cell Death-Inducing Activity

Yamaji¹,

Yamaguchi²,

Mitsuki³

et al. 2012

Modeling and Simulation in Engineering

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“…The research on oligosaccharides had developed slowly owing to their complicated structures and the difficulty in predicting their functions. In recent years, research on oligosaccharide has been developed and its application to industrial materials has been attempted [1][2][3][4][5] . The functional roles of oligosaccharides on the cell surface have been extensively investigated.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Sphingolipid Biosynthesis in Cultured Cells Enhances the Oligosaccharide Production from Exogenous Artifical Substrate

Kato¹,

Kasuya²,

Hatanaka³

2008

American J. of Biochemistry and Biotechnology

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The effect of fumonisin B1 which is an inhibitor of ceramide synthesis on saccharide elongation of dodecyl β-lactoside (Lac-C12), which is the analogue of lactosyl ceramide (LacCer), in mouse melanoma B16 cells and African green-monkey kidney (Vero) cells was examined. By adding fumonisin B1 in the culture medium used for glycosylation of Lac-C12, mouse melanoma B16 cells synthesized GM3-type analogue twice much as the absence of fumonisin B₁, while the amount of endogenous GM3 remarkably decreased. The addition of fumonisin B₁ to the culture medium of Vero cells also elicited 50% augmentation in the amount of glycosylated Lac-C12. For the enhanced production of oligosaccharide using cell function, fumonisin B₁ acts effectively on cells which produce abundant endogenous glycolipid. The addition of fumonisin B1 resulted in an increase in production of glycosylated Lac-C12 with decreasing natural ceramide synthesis

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Application of a Multivalent Glycoprobe: Characterization of Sugar-Binding Specificity of Siglec Family Proteins

Cited by 6 publications

References 8 publications

Integrative Analysis of Siglec-15 mRNA in Human Cancers Based on Data Mining

Integrative Analysis of Siglec-15 mRNA in Human Cancers Based on Data Mining

3D Modeling of a Natural Killer Receptor, Siglec-7: Critical Amino Acids for Glycan-Binding and Cell Death-Inducing Activity

Inhibition of Sphingolipid Biosynthesis in Cultured Cells Enhances the Oligosaccharide Production from Exogenous Artifical Substrate

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