Application of a multiclass screening method for veterinary drugs and pesticides using HPLC-QTOF-MS in egg samples

Hou, Xue; Xu, Xu; Xu, Xiao‐Ying; Han, Mei; Qiu, Shiting

doi:10.1016/j.foodchem.2019.125746

Cited by 41 publications

(10 citation statements)

References 30 publications

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“…This may explain the fact that published multiresidue analytical methods to quantify veterinary pharmaceutical levels in animal samples usually focus on a group of compounds (for a review, see [25]). Nevertheless, there is an increasing number of published methods for qualitative and quantitative analyses of veterinary drugs of different chemical classes in these complex matrices [26][27][28]. In addition, another challenge in veterinary drug residue analysis is that these contaminants usually occur in trace concentrations, frequently below limits of detection of most analytical instruments [25].…”

Section: Introductionmentioning

confidence: 99%

Validation of Multi-Residue Method for Quantification of Antibiotics and NSAIDs in Avian Scavengers by Using Small Amounts of Plasma in HPLC-MS-TOF

Gómez‐Ramírez

Blanco

García-Fernández

2020

IJERPH

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Pharmaceuticals are still considered emerging pollutants affecting both aquatic and terrestrial ecosystems. Scavenging bird species may be exposed to veterinary drugs when they feed on livestock carcasses provided at supplementary feeding stations, as these are often stocked with ailing and/or recently medicated animals. Because those animals may be a source of several different pharmaceutical compounds, analytical methods to evaluate residue levels and exposure potential should enable detection and quantification of as many different compounds as possible, preferably from small sample volumes. Four different extraction methods were tested to conduct HPLC-MS-TOF analysis of some of the most common veterinary drugs used in livestock in Spain. The method deemed most viable was a simple extraction, using methanol and 100 µL of plasma, that allowed quantification of seven antibiotics (tetracycline, oxytetracycline, ciprofloxacin, enrofloxacin, nalidixic acid, trimethoprim, sulfadiazine) and five nonsteroidal anti-inflammatory drugs (NSAIDs) (meloxicam, flunixin, carprofen, tolfenamic acid, phenylbutazone). The method was then applied to analysis of 29 Eurasian griffon vulture (Gyps fulvus) nestling samples, wherein enrofloxacin and tolfenamic acid were most commonly detected (69% and 20%, respectively). To our knowledge, this is the first study including NSAIDs in the exposure assessment of different classes of veterinary pharmaceuticals in live avian scavengers.

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Section: Introductionmentioning

confidence: 99%

Validation of Multi-Residue Method for Quantification of Antibiotics and NSAIDs in Avian Scavengers by Using Small Amounts of Plasma in HPLC-MS-TOF

Gómez‐Ramírez

Blanco

García-Fernández

2020

IJERPH

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“…Compared with TOF mode, the use of the SRMHS enhanced mode for sample quantification is more suitable for regulated bioanalysis. Hou et al (2020) used high performance liquid chromatography (HPLC) Q-TOF mass spectrometer to screen veterinary drugs and pesticides in eggs. Sulfachloropyridazine, sulfamethoxine, and fipronil sulfone were detected.…”

Section: Combination Of Quadrupole and Tof (Q-tof)mentioning

confidence: 99%

Towards Higher Sensitivity of Mass Spectrometry: A Perspective From the Mass Analyzers

Li¹,

Chu²,

Tan³

et al. 2021

Front. Chem.

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Mass spectrometry (MS) is one of the most widely used analytical techniques in many fields. Recent developments in chemical and biological researches have drawn much attention to the measurement of substances with low abundances in samples. Continuous efforts have been made consequently to further improve the sensitivity of MS. Modifications on the mass analyzers of mass spectrometers offer a direct, universal and practical way to obtain higher sensitivity. This review provides a comprehensive overview of the latest developments in mass analyzers for the improvement of mass spectrometers’ sensitivity, including quadrupole, ion trap, time-of-flight (TOF) and Fourier transform ion cyclotron (FT-ICR), as well as different combinations of these mass analyzers. The advantages and limitations of different mass analyzers and their combinations are compared and discussed. This review provides guidance to the selection of suitable mass spectrometers in chemical and biological analytical applications. It is also beneficial to the development of novel mass spectrometers.

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“…Fipronil is a broad-spectrum phenylpyrazole insecticide and acaricide that is commonly applied to plants and poultry or used as a soil pesticide . Owing to its widespread use, fipronil and its metabolites (FPM) have been detected in crops, vegetables, fruits, and eggs, as well as in environmental samples, including surface water, groundwater, drinking water, and indoor dust, , sampled from many countries worldwide. Previous studies have demonstrated that drinking water is one of the most important sources of human exposure to fipronil .…”

Section: Introductionmentioning

confidence: 99%

Thyroid-Disrupting Effects of Exposure to Fipronil and Its Metabolites from Drinking Water Based on Human Thyroid Follicular Epithelial Nthy-ori 3-1 Cell Lines

Zhuang

Jiang

Chen

et al. 2023

Environ. Sci. Technol.

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Fipronil is a broad-spectrum insecticide used for plants and poultry. Owing to its widespread use, fipronil and its metabolites (fipronil sulfone, fipronil desulfinyl, and fipronil sulfide), termed FPM, can be frequently detected in drinking water and food. Fipronil can affect the thyroid function of animals, but the effects of FPM on the human thyroid remain unclear. We employed human thyroid follicular epithelial Nthy-ori 3-1 cells to examine combined cytotoxic responses, thyroid-related functional proteins including the sodium-iodide symporter (NIS), thyroid peroxidase (TPO), deiodinases I−III (DIO I−III), and the nuclear factor erythroid-derived factor 2−related factor 2 (NRF2) pathway induced by FPM of 1−1000-fold concentrations detected in school drinking water collected from a heavily contaminated area of the Huai River Basin. Thyroid-disrupting effects of FPM were evaluated by examining biomarkers of oxidative stress and thyroid function and tetraiodothyronine (T4) levels secreted by Nthy-ori 3-1 cells after FPM treatment. FPM activated the expression of NRF2, HO-1 (heme oxygenase 1), TPO, DIO I, and DIO II but inhibited NIS expression and increased the T4 level of thyrocytes, indicating that FPM can disrupt the function of human thyrocytes through oxidative pathways. Given the adverse impact of low FPM concentrations on human thyrocytes, supportive evidence from rodent studies, and the critical importance of thyroid hormones on development, the effects of FPM on the neurodevelopment and growth of children warrant priority attention.

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Application of a multiclass screening method for veterinary drugs and pesticides using HPLC-QTOF-MS in egg samples

Cited by 41 publications

References 30 publications

Validation of Multi-Residue Method for Quantification of Antibiotics and NSAIDs in Avian Scavengers by Using Small Amounts of Plasma in HPLC-MS-TOF

Validation of Multi-Residue Method for Quantification of Antibiotics and NSAIDs in Avian Scavengers by Using Small Amounts of Plasma in HPLC-MS-TOF

Towards Higher Sensitivity of Mass Spectrometry: A Perspective From the Mass Analyzers

Thyroid-Disrupting Effects of Exposure to Fipronil and Its Metabolites from Drinking Water Based on Human Thyroid Follicular Epithelial Nthy-ori 3-1 Cell Lines

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