Application of a Microtechnique to the Agglutination Test for Leptospiral Antibodies1

Galton, Mildred M.; Sulzer, Catherine R.; Rosa, C. A. Santa; Fields, Michael J.

doi:10.1128/aem.13.1.81-85.1965

Cited by 79 publications

(38 citation statements)

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“…The serological diagnosis of leptospirosis was performed using the MAT assay, according to Galton et al (1965) and Cole et al (1973). The tests used a collection of live antigens represented by the serogroups: Icterohaemorrhagiae (serovars Icterohaemorrhagiae and Copenhageni), Canicola, Pomona, Grippotyphosa, Serjoe (Wolffi, Hardjoprajitno, Hardjobovis and Guaricura), Australis (Australis and Bratislava), Andamana, Autumnalis (Autumnalis and Butembo), Bataviae, Balum (Castellonis), Cynopteri, Djasiman (Sentot), Hebdomadis, Panama, Tarassovi, Javanica, Celledoni (Whitcombi), Pyrogenes, Shermani, and Seramanga (Patoc).…”

Section: Serological Diagnosis Of Leptospira Spmentioning

confidence: 99%

Seroprevalence and predominant serogroups of Leptospira sp. in serological tests of ruminants in northeastern Brazil

Pimenta

Bezerra

Morais

et al. 2019

SCA

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The objective of this study was to determine the seroprevalence of leptospirosis and the predominant serogroups in serological tests of ruminants performed at the Transmissible Diseases Laboratory of the Federal University of Campina Grande, Patos, Paraíba, northeastern Brazil, between 2010 and 2017. This research included the records of serological tests for leptospirosis from 5594 animals, including 1527 cattle, 1761 goats, 2170 sheep, and 136 buffaloes from four Brazilian states (Paraíba, Pernambuco, Maranhão, and Rio Grande do Norte). Of the 5594 serum samples, 662 were serologically positive, corresponding to a frequency of 11.8%. Serjoe (30.6%), Autumnalis (13.6%), and Icterohaemorrhagiae (11.3%) were the most frequent subgroups among all species. The individual frequencies in cattle, goats, sheep, and buffaloes were 20% (306/1527), 8.3% (147/1761), 7.9% (171/2170), and 27.9% (38/136), respectively, with titers ranging from 1:100 to 1:3200. The most frequent serogroups according to animal species were as follows: Serjoe predominated in cattle (62%), followed by Icterohaemorrhagiae (12.5%) and Tarassovi (6.6%); Autumnalis was the most frequent in goats and sheep (29.4% and 26.9%, respectively), followed by Seramanga (12.5%) in goats and Icterohaemmorrhagiae (13.5%) in sheep; and Australis predominated in buffaloes (39.5%), followed by Pomona (31.6%) and Canicola (21.1%). Leptospira sp. infection, determined by serology, is widespread in ruminants (cattle, goats, sheep, and buffaloes) in northeastern Brazil, suggesting the existence of alternative transmission routes that are less dependent on environmental factors. Identification of the most frequent serogroups highlights the need to improve sanitary conditions, and implement efficient and targeted control measures directed at the primary infection sources.

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Section: Serological Diagnosis Of Leptospira Spmentioning

confidence: 99%

Seroprevalence and predominant serogroups of Leptospira sp. in serological tests of ruminants in northeastern Brazil

Pimenta

Bezerra

Morais

et al. 2019

SCA

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“…Serological diagnosis of Leptospira spp. infection were carried out in the Laboratory of Bacterial Reproductive Diseases, of the Biological Institute of São Paulo, through the microscopic serum-agglutination (MAT) test, according to Galton et al (1965) and Cole et al (1973), with a collection of living antigens that included the following serovars: Icterohaemorrhagiae, Canicola, Pomona, Grippotyphosa, Wolffi, Hardjo, Australis, Autumnalis, Bataviae, Bratislava, Butembo, Castellonis, Copenhageni, Cynopteri, Hebdomadis, Javanica, Panama, Pyrogenes, Shermani, Tarassovi, Whitcombi, Sentot and Mini. Sera were screened under the dilution of 1:100, and those presenting 50.0% or more of agglutination were titrated by means of a series of geometrical dilutions at the rate of two.…”

Section: Samples Harvest and Processingmentioning

confidence: 99%

Prevalence and Risk Factors Associated withLeptospiraspp. Infection in Technified Swine Farms in the State of Alagoas, Brazil Risk Factors Associated withLeptospiraspp. in Swine Farms

Valença¹,

Mota

Castro

et al. 2012

Transbound Emerg Dis

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Summary The aim of this study was to determine the prevalence and to identify the risk factors associated with Leptospira spp. infection in technified pig farms in the state of Alagoas, Brazil. To compose sample for the prevalence study, 342 pigs were used (312 sows and 30 boars) proceeding from seven swine farms distributed in five districts of the state of Alagoas, Brazil. The infection’s serological diagnosis was performed by microscopic agglutination test. The risk factors analysis was performed using research questionnaires consisting of objective questions related to the breeder, the general characteristics of the property, and the productive, reproductive and sanitary management. Prevalence of 16.1% (55/342) of pigs seropositive was obtained. The associated risk factors were not performing quarantine (P = 0.003, OR = 5.43, CI = 1.79–16.41) and the use of artificial insemination (P = 0.023, OR = 3.38, CI = 1.18–9.66). A significant association of sow infection with the increased number of stillborn and mummified foetuses was found, as well as with the increased frequency of oestrus recurrence and the increased weaning‐to‐oestrus interval of seropositive sows. One might state that Leptospira spp. infection is disseminated in technified pig farms in the State of Alagoas, favouring reproductive failures and the impairment of zootechnical performance in these properties. The risk factors identified in this study are facilitators in the infecting agent dissemination and should be adjusted to control the disease in the herds studied.

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“…The MSA test was performed at the Laboratory of Reproductive Bacterial Diseases of the Biological Institute of São Paulo. The technique was performed according to Galton et al 15 and Cole et al 16 using live cultures of Leptospira spp. of serovars Icterohaemorrhagiae, Canicola, Pomona, Grippotyphosa, Wolffi, Hardjo, Australis, Autumnalis, Bataviae, Bratislava, Butembo, Castellonis, Copenhageni, Cynopteri, Hebdomadis, Javanica, Panama, Pyrogenes, Shermani, Tarassovi, Whitcombi, Sentot as antigens.…”

Section: Determination Of Anti-leptospira Agglutinin Levelsmentioning

confidence: 99%

Análise sociodemográfica e ambiental para ocorrência de anticorpos antiLeptospira em cães de Teresina, Piauí, Brasil

Silva

Castro

Mineiro

et al. 2018

Ciênc. saúde coletiva

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Leptospirosis is a worldwide zoonosis whose transmission is interlinked by multiple factors in the man-animal-ecosystem interface. This study aimed to evaluate the risk factors for the occurrence of anti-Leptospira antibodies in dogs in the capital Teresina (PI), and to determine their spatial distribution. Five hundred fifty-eight dog blood samples were submitted to the Microscopic Serum Agglutination (MSA) test. We applied semi-structured questionnaires to dog owners and obtained the area of residence for projection in geographical maps. Serum prevalence was 13.8%, in which the most common serovar was icterohaemorrhagiae, with 49.2%. Dogs with street access, failure to collect food bowl and low income of owners were risk factors. There was a higher number of seropositive dogs in the rainy season, with 87.1%, which is a probable risk factor for the occurrence of cases. The distribution of seropositive dogs was widely spread in the city, with predominance of cases in anthropized areas. These risk factors favor the occurrence of anti-Leptospira antibodies in dogs that are agent maintenance sources in the city and reinforce the need for epidemiological and environmental surveillance to prevent leptospirosis.

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Application of a Microtechnique to the Agglutination Test for Leptospiral Antibodies1

Cited by 79 publications

References 0 publications

Seroprevalence and predominant serogroups of Leptospira sp. in serological tests of ruminants in northeastern Brazil

Seroprevalence and predominant serogroups of Leptospira sp. in serological tests of ruminants in northeastern Brazil

Prevalence and Risk Factors Associated withLeptospiraspp. Infection in Technified Swine Farms in the State of Alagoas, Brazil Risk Factors Associated withLeptospiraspp. in Swine Farms

Análise sociodemográfica e ambiental para ocorrência de anticorpos antiLeptospira em cães de Teresina, Piauí, Brasil

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