Application of a microfluidic paper-based bioimmunosensor with laser-induced fluorescence detection in the determination of alpha-fetoprotein from serum of hepatopaths

Wang, Wei; Cai, Xiaoyu; Li, Qingling; Zheng, Lili; Yu, Xiufeng; Zhang, Huimin; Wang, Jian

doi:10.1016/j.talanta.2020.121660

Cited by 22 publications

(11 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A λ exonuclease-assisted paper-based FL assay [ 179 ] was described for facile testing of polynucleotide kinase (PNK) activity by fluorescence intensity on paper surfaces, achieving sensitivity of PNK activity down to 0.0001 U/mL. In addition, paper-based FL immunoassays were used commonly for the detection of antibody biomarker [ 182 – 185 ]. CdTe/CdSe QD and relevant enzyme were saturated on paper.…”

Section: Optical Detection Methodsmentioning

confidence: 99%

“…a Fluorescence "off-to-on" mechanism on the GO paper for F − detecting [163]; b Schematic illustration of the tricolor probe for Cu 2+ [169]; c Illustration of distance-dependent immunoassay on μPAD [177]; d Schematic illustra-tion for FL detection of CEA on μPAD [182]; e Schematic illustration of portable test strips and wearable devices for the analysis of TC [199]; f Schematic illustrations of a single dual-emissive ratiometric probe for thiram by smartphone [203] kinase (PNK) activity by fluorescence intensity on paper surfaces, achieving sensitivity of PNK activity down to 0.0001 U/mL. In addition, paper-based FL immunoassays were used commonly for the detection of antibody biomarker [182][183][184][185]. CdTe/CdSe QD and relevant enzyme were saturated on paper.…”

Section: Biochemical Analysismentioning

confidence: 99%

See 1 more Smart Citation

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Hou

Guo

et al. 2022

J. Anal. Test.

View full text Add to dashboard Cite

Point-of-care testing (POCT), as a portable and user-friendly technology, can obtain accurate test results immediately at the sampling point. Nowadays, microfluidic paper-based analysis devices (μPads) have attracted the eye of the public and accelerated the development of POCT. A variety of detection methods are combined with μPads to realize precise, rapid and sensitive POCT. This article mainly introduced the development of electrochemistry and optical detection methods on μPads for POCT and their applications on disease analysis, environmental monitoring and food control in the past 5 years. Finally, the challenges and future development prospects of μPads for POCT were discussed.

show abstract

Section: Optical Detection Methodsmentioning

confidence: 99%

Section: Biochemical Analysismentioning

confidence: 99%

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Hou

Guo

et al. 2022

J. Anal. Test.

View full text Add to dashboard Cite

show abstract

“…The final product is usually a long double-stranded DNA (dsDNA), which provides excellent signal amplification efficiency. 17 In contrast to traditional polymerase chain reaction (PCR), HCR is conducted at a constant temperature and avoids the use of any enzymes, which is much suitable for the analysis of temperature-sensitive targets like proteins, 18 exosomes, 19 and cells. 20 The reaction process is simplified with mild conditions, thus good practical use is verified.…”

Section: Introductionmentioning

confidence: 99%

Recent Progress in DNA Hybridization Chain Reaction Strategies for Amplified Biosensing

Chai

Cheng

Jin

et al. 2021

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

With the continuous development of DNA nanotechnology, various spatial DNA structures and assembly techniques emerge. Hybridization chain reaction (HCR) is a typical example with exciting features and bright prospects in biosensing, which has been intensively investigated in the past decade. In this Spotlight on Applications, we summarize the assembly principles of conventional HCR and some novel forms of linear/nonlinear HCR. With advantages like great assembly kinetics, facile operation, and an enzyme-free and isothermal reaction, these strategies can be integrated with most mainstream reporters (e.g., fluorescence, electrochemistry, and colorimetry) for the ultrasensitive detection of abundant targets. Particularly, we select several representative studies to better illustrate the novel ideas and performances of HCR strategies. Theoretical and practical utilities are confirmed for a range of biosensing applications. In the end, a deep discussion is provided about the challenges and future tasks of this field.

show abstract

“…Early diagnosis through cancer biomarker is crucial for cancer monitoring, improvement of efficacy and survival rate 2 . Among the cancer biomarkers, Alpha-fetoprotein (AFP, a kind of glycoprotein) is widely applied for the diagnosis of many cancers 3 . Due to the high selectivity, immunoassays-based methods are the predominant for AFP determination, such as enzyme-linked immunosorbent assay (ELISA), radioimmunoassay and electrochemiluminescence immunoassay [3][4][5] .…”

Section: Introductionmentioning

confidence: 99%

“…Among the cancer biomarkers, Alpha-fetoprotein (AFP, a kind of glycoprotein) is widely applied for the diagnosis of many cancers 3 . Due to the high selectivity, immunoassays-based methods are the predominant for AFP determination, such as enzyme-linked immunosorbent assay (ELISA), radioimmunoassay and electrochemiluminescence immunoassay [3][4][5] . However, traditional immunoassays are time-consuming and costly, and require professional operators and complicated operation steps 6,7 .…”

Section: Introductionmentioning

confidence: 99%

One-sampling and Rapid Analysis of Cancer Biomarker on a Power-free and Low-cost Microfluidic Chip

et al. 2021

View full text Add to dashboard Cite

Alpha-fetoprotein (AFP) is an important disease biomarker, related to cancers such as hepatocarcinomas and gastric cancer. However, traditional methods are time-consuming, relied on bulky instruments and trained professionals, cannot satisfy the demand for low cost and point-of-care testing (POCT). In this study, a power-free POCT device is developed for rapid and low-cost detection of AFP via one-sampling. Based on the principle of sandwich immunofluorescence, the chip is capable of automatically accomplishing on-chip mixing, labeling and capturing procedures, which only requires the operator add 40 μl sample into the chip one time. The proposed device is capable of sensitively detecting human AFP in FBS with a dynamic ranging from 10-1 000 ng/mL and LOD (1.88 ng/ml) within a short time of 3 min.Predictably, our method holds the great potential to be applied in POC diagnostics of proteins, especially for some regions with resource-limited.

show abstract

Application of a microfluidic paper-based bioimmunosensor with laser-induced fluorescence detection in the determination of alpha-fetoprotein from serum of hepatopaths

Cited by 22 publications

References 25 publications

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Recent Progress in DNA Hybridization Chain Reaction Strategies for Amplified Biosensing

One-sampling and Rapid Analysis of Cancer Biomarker on a Power-free and Low-cost Microfluidic Chip

Contact Info

Product

Resources

About