Application of a micro-droplet generator for an ICP-sector field mass spectrometer – optimization and analytical characterization

Acquisition of fast transient signals in ICP-MS with enhanced time resolution

“…The ICP-MS was operated according to a previously reported fast-transient data acquisition protocol. 25 In brief, this used low resolution mode, the E-scan setting, pulse counting detection mode, an integration time of 100 µs, 1000 integrations or "samples" per peak, and a mass window of 20%. One isotope ( 197 Au) was analysed per run.…”

Time-resolved ICP-MS Measurement: a New Method for Elemental and Multiparametric Analysis of Single Cells

“…Therefore, the amount distribution of the elements in single cells, including the cell-to-cell variations, can be evaluated. 17,18 Through combination with elemental tagging techniques, the amount distributions of biomolecules in individual cells can also be evaluated similar to a flow cytometer. [19][20][21][22][23] Time-resolved measurement is not a new technique for ICP optical emission spectrometry (ICP-OES) and ICP-MS, but it has been mainly applied to the elemental analysis of airborne particle matter [31][32][33][34] and observation studies of particle decomposition inside the plasma.…”

“…Not only metal-containing biomolecules, but also other biomolecules, such as amino acids, peptides, proteins, nucleic acids and microbial cells, can be successfully quantified by measuring heteroatoms, such as phosphorus and sulfur, and/or by supporting elemental tags, such as metal-labeled antigens and nanoparticles. [4][5][6][7][8][9][10] In recent years, cytometric analysis of single cells with a time-resolved ICP-MS measurement is receiving much attention for elemental [14][15][16][17][18] and multiparametric [19][20][21][22][23] analyses of single cells. Due to cellular heterogeneity within an isogenic cell population, individual analysis of cells will lead to a more sensitive representation of cell-to-cell variations, instead of a stochastic average analysis by bulk measurements.…”

“…[24][25][26][27][28][29] Classical ICP-MS analysis of cells is the bulk analysis of large amounts of cells with a lysis, extraction or digestion; 30 this is an approach that is hardly adapted for evaluating any cell-to-cell variance. Time-resolved measurement via a direct cell introduction method is a relatively recent method for individual analysis of cells by ICP-MS, [14][15][16][17][18][19][20][21][22][23] an approach that can detect the elements in individual cells with a high sensitivity, often called "single particle mode". When cells are directly introduced into the plasma, ion plumes of the cells are generated after their vaporization.…”

Development of a High-Density Microplasma Emission Source for a Micro Total Analysis System

“…12 In this method, a plasma with a volume of approximately 10 cm 3 excites and ionizes the samples. Recently, a microplasma, where the characteristic length of the plasma is on the order of micrometers, has attracted the attention of researchers in various fields; it has been used for hydrophilization of the surfaces of films, 13 and in medical applications for sterilization 14,15 and for atmospheric pressure desorption ionization.…”