Application of a fluorometric microplate algal toxicity assay for riverine periphytic algal species

Nagai, Takashi; Taya, Kiyoshi; Annoh, Hirochica; Ishihara, Satoru

doi:10.1016/j.ecoenv.2013.04.020

Cited by 22 publications

(45 citation statements)

References 25 publications

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“…To test a lot of mixture combinations simultaneously, an efficient test method is essential. Therefore, fluorimetric microplate algal toxicity assays [12] were conducted according to the standardized algal growth inhibition test [10]. Preculturing was performed under the Acc e p ted P r e p r i nt by in-vivo fluorescence using a microplate reader (Infinite M200, Tecan).…”

Section: Fluorescence Microplate Toxicity Assaymentioning

confidence: 99%

The effect of binary mixtures of zinc, copper, cadmium, and nickel on the growth of the freshwater diatom Navicula pelliculosa and comparison with mixture toxicity model predictions

Nagai

Schamphelaere

2016

Enviro Toxic and Chemistry

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The authors investigated the effect of binary mixtures of zinc (Zn), copper (Cu), cadmium (Cd), and nickel (Ni) on the growth of a freshwater diatom, Navicula pelliculosa. A 7 × 7 full factorial experimental design (49 combinations in total) was used to test each binary metal mixture. A 3-d fluorescence microplate toxicity assay was used to test each combination. Mixture effects were predicted by concentration addition and independent action models based on a single-metal concentration-response relationship between the relative growth rate and the calculated free metal ion activity. Although the concentration addition model predicted the observed mixture toxicity significantly better than the independent action model for the Zn-Cu mixture, the independent action model predicted the observed mixture toxicity significantly better than the concentration addition model for the Cd-Zn, Cd-Ni, and Cd-Cu mixtures. For the Zn-Ni and Cu-Ni mixtures, it was unclear which of the 2 models was better. Statistical analysis concerning antagonistic/synergistic interactions showed that the concentration addition model is generally conservative (with the Zn-Ni mixture being the sole exception), indicating that the concentration addition model would be useful as a method for a conservative first-tier screening-level risk analysis of metal mixtures. Environ Toxicol Chem 2016;35:2765-2773. © 2016 SETAC.

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Section: Fluorescence Microplate Toxicity Assaymentioning

confidence: 99%

The effect of binary mixtures of zinc, copper, cadmium, and nickel on the growth of the freshwater diatom Navicula pelliculosa and comparison with mixture toxicity model predictions

Nagai

Schamphelaere

2016

Enviro Toxic and Chemistry

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“…Therefore, filling the data gap in an efficient and economical way would be essential to enhance the SSD application. 3,42) Monitoring the actual environmental concentration of pesticides and risk assessment after registration is also important for risk management. Quantification of the ecological risk of pesticides using SSD as the PAF provides a useful tool for risk management.…”

Section: Application and Perspectivementioning

confidence: 99%

Ecological effect assessment by species sensitivity distribution for 68 pesticides used in Japanese paddy fields

Nagai

2016

J. Pestic. Sci.

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In this study, the species sensitivity distributions (SSDs) of 68 pesticides commonly used in Japanese paddy fields were analyzed based on collected acute toxicity data as a higher-tier ecological effect assessment. Then, based on each SSD, the 5% hazardous concentration (HC 5 ) values were calculated as the predicted no-effect concentrations for aquatic ecosystems. The differences between HC 5 and registration criteria were small (within 10-fold) for 50 of the 68 pesticides. However, there were more than 10-fold differences for 9 insecticides and 9 herbicides of specific modes of action. These results suggest that the current effect assessment scheme could underestimate the effect of such pesticides. This is caused by the mode of action specific properties of species sensitivity difference.

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“…While, there have been previous chemical inhibition studies with algae (Nagai et al 2013;Blaise and Vasseur 2005;Eisentraeger et al 2003), typically in a microplate format, this methodology is not applicable when working with volatile chemicals. Without a sealed environment, the chemicals would not stay in the aqueous phase.…”

Section: Discussionmentioning

confidence: 95%

Optimizing cyanobacteria growth conditions in a sealed environment to enable chemical inhibition tests with volatile chemicals

Johnson

Zahler

Baldwin

et al. 2016

Journal of Microbiological Methods

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Application of a fluorometric microplate algal toxicity assay for riverine periphytic algal species

Cited by 22 publications

References 25 publications

The effect of binary mixtures of zinc, copper, cadmium, and nickel on the growth of the freshwater diatom Navicula pelliculosa and comparison with mixture toxicity model predictions

The effect of binary mixtures of zinc, copper, cadmium, and nickel on the growth of the freshwater diatom Navicula pelliculosa and comparison with mixture toxicity model predictions

Ecological effect assessment by species sensitivity distribution for 68 pesticides used in Japanese paddy fields

Optimizing cyanobacteria growth conditions in a sealed environment to enable chemical inhibition tests with volatile chemicals

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