Abstract:A construção e a aplicação de um sensor biomimético para a determinação de 4-metilbenzilideno cânfora (4-MBC), um protetor de radiação ultravioleta (UV), são descritas. O sensor foi preparado pela modificação de um eletrodo de pasta de carbono com um complexo de cloreto de ferro(III) com ftalocianina, FePcCl. As medidas amperométricas conduzidas com o sensor, sob um potencial aplicado de 0,0 V vs. Ag|AgCl em uma mistura de tetraidrofurano e 0,1 mol L -1 H 2 SO 4 (30:70 em volume), mostraram uma resposta linear… Show more
“…All the chemicals used were of analytical or HPLC grade. Copper(II) 1,2,3,4,8,9,10,11,15,16,17,18,22,23,24,25-hexadecauoro 29H,31H-phthalocyanine [CuPc], bumetanide, mineral oil and graphite powder were purchased from Sigma-Aldrich.…”
Section: Reagents and Solutionsmentioning
confidence: 99%
“…The graph obtained by plotting the current of the oxidation peak of square wave voltammograms versus the concentration including saturated amounts of the analyte should be similar to that of an enzymatic biosensor, since the complex used had a structure similar to that of the P450 hemeenzymes. In the case of the biosensor based on redox enzymes, the current signal initially increases linearly with the concentration of the substrate ([S]), and then, as [S] is further increased, the enzyme becomes saturated, and the current signal reaches a maximum value 23,27 producing a graph with a hyperbolic prole. A biomimetic sensor, just as ours, should therefore produce the same graphic, which means the sensor response follows a pseudo-Michaelis-Menten kinetics, which is expected, since our catalyst is based on the structure of the active site of a redox enzyme.…”
Section: Evaluation Of the Biomimetic Characteristics Of The Sensormentioning
confidence: 99%
“…18 In addition, the degradation of xenobiotics such as drugs, pesticides and endocrine disruptors is possible through hydroxylation, oxidation or reduction reactions. 18,19 Materials derived from the phthalocyanines and porphyrins of iron and other metals have been successfully used in the construction of biomimetic chemical sensors for analytical use, [20][21][22][23][24] since these compounds mimic the chemical structure of the P450 active site (iron protoporphyrin IX).…”
This paper describes the development of a new method for the quantification of bumetanide in urine samples from athletes and in pharmaceutical formulations to detect doping, using a biomimetic sensor based on a biomimetic catalyst of the P450 enzyme.
“…All the chemicals used were of analytical or HPLC grade. Copper(II) 1,2,3,4,8,9,10,11,15,16,17,18,22,23,24,25-hexadecauoro 29H,31H-phthalocyanine [CuPc], bumetanide, mineral oil and graphite powder were purchased from Sigma-Aldrich.…”
Section: Reagents and Solutionsmentioning
confidence: 99%
“…The graph obtained by plotting the current of the oxidation peak of square wave voltammograms versus the concentration including saturated amounts of the analyte should be similar to that of an enzymatic biosensor, since the complex used had a structure similar to that of the P450 hemeenzymes. In the case of the biosensor based on redox enzymes, the current signal initially increases linearly with the concentration of the substrate ([S]), and then, as [S] is further increased, the enzyme becomes saturated, and the current signal reaches a maximum value 23,27 producing a graph with a hyperbolic prole. A biomimetic sensor, just as ours, should therefore produce the same graphic, which means the sensor response follows a pseudo-Michaelis-Menten kinetics, which is expected, since our catalyst is based on the structure of the active site of a redox enzyme.…”
Section: Evaluation Of the Biomimetic Characteristics Of The Sensormentioning
confidence: 99%
“…18 In addition, the degradation of xenobiotics such as drugs, pesticides and endocrine disruptors is possible through hydroxylation, oxidation or reduction reactions. 18,19 Materials derived from the phthalocyanines and porphyrins of iron and other metals have been successfully used in the construction of biomimetic chemical sensors for analytical use, [20][21][22][23][24] since these compounds mimic the chemical structure of the P450 active site (iron protoporphyrin IX).…”
This paper describes the development of a new method for the quantification of bumetanide in urine samples from athletes and in pharmaceutical formulations to detect doping, using a biomimetic sensor based on a biomimetic catalyst of the P450 enzyme.
“…The biomimetic properties of the sensor were reflected in a response plot that showed a hyperbolic profile for acetazolamide concentrations between 1.00 Â 10 À 6 mol L À 1 and 1.00 Â 10 À 5 mol L À 1 (Supplementary material). In such cases, the reaction rate increases with increasing substrate concentration and must asymptotically approach the saturation rate that is directly proportional to the total biomimetic material concentration (Boni et al, 2010;Ruy et al, 2014). Similarly, for concentrations higher than 5.00 Â 10 À 6 mol L À 1 , the proposed bio-inspired sensor showed marked deviation from linearity, which could be attributed to the maximum saturation of the electrocatalytic sites on the electrode surface.…”
Section: Evaluation Of Biomimetic Characteristicsmentioning
A bio-inspired electrochemical sensor using a binuclear oxo-manganese complex was evaluated and applied in the detection of a substance associated with doping in sports: acetazolamide (ACTZ). Investigation was made of the influence of different experimental variables on the electrocatalytic oxidation of ACTZ by the bio-inspired sensor, such as pH and interfering species. The bio-inspired sensor showed the best response in the range from 5.00×10(-9) to 7.00×10(-8) mol L(-1) ACTZ, with a linear range from 5.00×10(-9) to 2.50×10(-8) mol L(-1) and a detection limit of 4.76×10(-9) mol L(-1). The sensor exhibited characteristics similar to the Michaelis-Menten model of an enzymatic electrode, due to the use of a multinucleated complex of manganese with μ-oxo units, which was able to mimic the properties of enzymes with manganese as a cofactor in their composition, such as Mn-containing oxidase. The determination of ACTZ with the bio-inspired sensor was evaluated using three different synthetic biological fluids (plasma, saliva, and urine), demonstrating its viability for use with real samples. The analysis of ACTZ in real urine samples using the bio-inspired sensor, simulating the method adopted by the World Anti-Doping Agency, which revealed viable, suggesting a new and promising platform to be used in these analysis.
“…Biomimetic sensors are more sensitive, compared to enzymatic biosensors, due to a smaller diffusion barrier and greater electron transfer between the biomimetic complex and the analyte [6,7]. This new and increasingly popular class of sensors has been used for the identification and quantification of various drugs [13][14][15][16][17], and has also been coupled to flow systems [18,19].…”
A biomimetic sensor for the determination of dipyrone was prepared by modifying carbon paste with cobalt phthalocyanine (CoPc), and used as an amperometric detector in a flow injection analysis (FIA) system. The results of cyclic voltammetry experiments suggested that CoPc behaved as a biomimetic catalyst in the electrocatalytic oxidation of dipyrone, which involved the transfer of one electron. The optimized FIA procedure employed a flow rate of 1.5 mL min(-1), a 75 μL sample loop, a 0.1 mol L(-1) phosphate buffer carrier solution at pH 7.0 and amperometric detection at a potential of 0.3 V vs. Ag/AgCl. Under these conditions, the proposed method showed a linear response for dipyrone concentrations in the range 5.0 × 10(-6)-6.3 × 10(-3) mol L(-1). Selectivity and interference studies were carried out in order to validate the system for use with pharmaceutical and environmental samples. In addition to being environmentally friendly, the proposed method is a sensitive and selective analytical tool for the determination of dipyrone.
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