Application of 1H-NMR based metabolite fingerprinting and chemometrics for authentication of Curcuma longa adulterated with C. heyneana

Windarsih, Anjar; Rohman, Abdul; Swasono, Respati Tri

doi:10.1016/j.jarmap.2019.100203

Cited by 21 publications

(19 citation statements)

References 29 publications

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“…The instruments mostly used for the analysis operate at a frequency of 500–600 MHz. NMR is a versatile molecular spectroscopy technique because of its advantages, such as easy in-sample preparation, less solvent requirements (considered as green analytical chemistry), less time required for analysis, highly reproducible and highly robust, as well as it can be used for the analysis of heterogeneous samples simultaneously [ 26 ]. The most common technique used is proton-NMR ( 1 H-NMR) spectroscopy because it offers simplicity in sample preparation, fast analysis, and it can be used even for crude extract analysis [ 27 ].…”

Section: Molecular Spectroscopymentioning

confidence: 99%

The Application of Molecular Spectroscopy in Combination with Chemometrics for Halal Authentication Analysis: A Review

Rohman

Windarsih

2020

IJMS

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Halal is an Arabic term used to describe any components allowed to be used in any products by Muslim communities. Halal food and halal pharmaceuticals are any food and pharmaceuticals which are safe and allowed to be consumed according to Islamic law (Shariah). Currently, in line with halal awareness, some Muslim countries such as Indonesia, Malaysia, and Middle East regions have developed some standards and regulations on halal products and halal certification. Among non-halal components, the presence of pig derivatives (lard, pork, and porcine gelatin) along with other non-halal meats (rat meat, wild boar meat, and dog meat) is typically found in food and pharmaceutical products. This review updates the recent application of molecular spectroscopy, including ultraviolet-visible, infrared, Raman, and nuclear magnetic resonance (NMR) spectroscopies, in combination with chemometrics of multivariate analysis, for analysis of non-halal components in food and pharmaceutical products. The combination of molecular spectroscopic-based techniques and chemometrics offers fast and reliable methods for screening the presence of non-halal components of pig derivatives and non-halal meats in food and pharmaceutical products.

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Section: Molecular Spectroscopymentioning

confidence: 99%

The Application of Molecular Spectroscopy in Combination with Chemometrics for Halal Authentication Analysis: A Review

Rohman

Windarsih

2020

IJMS

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“…In addition, this method is powerful, fast, reproducible, requires minimal sample preparation, and could be used to analyze metabolite profiles with only a crude extract [ 16 , 17 ]. Combined with chemometrics such as principal component analysis (PCA), partial least square-discriminant analysis (PLS-DA), and orthogonal projections to latent structures-discriminant analysis (OPLS-DA), the huge datasets generated from 1 H-NMR measurements could be handled [ 18 , 19 ] and can be used to assess the authentication of C. xanthorrhiza extract.…”

Section: Introductionmentioning

confidence: 99%

The Authentication of Java Turmeric (Curcuma xanthorrhiza) Using Thin Layer Chromatography and 1H-NMR Based-Metabolite Fingerprinting Coupled with Multivariate Analysis

et al. 2020

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The identification of adulteration practices of medicinal plants used as herbal medicine is very important to ensure the quality, safety, and efficacy. In this study, thin layer chromatography (TLC) and proton nuclear magnetic resonance (1H-NMR)-based metabolite fingerprinting coupled with multivariate analysis were used for authentication of Curcuma xanthorrhiza extract from Curcuma aeruginosa. Curcumin contents obtained from C. xanthorrhiza extract from various regions were in the range of 0.74%–1.23%. Meanwhile, curcumin contents obtained from C. xanthorrhiza extract adulterated with 0%, 10%, 25%, 40%, 50%, and 75% of C. aeruginosa were 1.02%, 0.96%, 0.86%, 0.69%, 0.43%, and 0.27%, respectively. The decreasing of curcumin contents in adulterant concentrations of 40% and more in C. xanthorrhiza rhizome could indicate the adulteration with other rhizomes. Multivariate analysis of PCA (principal component analysis) using data set obtained from 1H-NMR spectra clearly discriminated pure and adulterated C. xanthorrhiza with C. aeruginosa. OPLS-DA (orthogonal projections to latent structures-discriminant analysis) successfully classified pure and adulterated C. xanthorrhiza with higher R2X (0.965), R2Y (0.958), and Q2(cum) (0.93). It can be concluded that 1H-NMR-based metabolite fingerprinting coupled with PCA and OPLS-DA offers an adequate method to assess adulteration practice and to evaluate the authentication of C. xanthorrhiza extracts.

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“…Several analytical methodologies have been proposed for the characterization, identification and determination of curcuminoids in turmeric samples [ 3 , 8 , 15 ]. Some of them are based on spectral data from ultraviolet (UV) [ 16 , 17 ], fluorescence [ 18 ], Fourier transform infrared (FTIR) [ 16 , 19 , 20 ] or nuclear magnetic resonance (NMR) [ 16 , 21 , 22 , 23 , 24 ] spectroscopies. Electrochemical characterizations and determinations of curcuminoids in turmeric samples by bare and modified electrodes [ 25 ], such as graphene-modified [ 26 ] and multiwalled carbon nanotube modified [ 27 ] electrodes, have also been reported.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, this can be a challenging task because of the sample matrix complexity when dealing with food products, especially due to the presence of unknown interfering compounds [ 50 ]. Apart from quantitative methods, instrumental signals such as chromatographic peak areas of known selected compounds are a good alternative for food characterization and classification, also providing chemical information related to bioactive substances [ 22 , 23 , 24 ]. In this regard, polyphenolic and curcuminoid profiling by HPLC-UV has been reported for the characterization of turmeric and curry samples [ 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization, Classification and Authentication of Turmeric and Curry Samples by Targeted LC-HRMS Polyphenolic and Curcuminoid Profiling and Chemometrics

et al. 2020

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The importance of monitoring bioactive substances as food features to address sample classification and authentication is increasing. In this work, targeted liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) polyphenolic and curcuminoid profiles were evaluated as chemical descriptors to deal with the characterization and classification of turmeric and curry samples. The profiles corresponding to bioactive substances were obtained by TraceFinderTM software using accurate mass databases with 53 and 24 polyphenolic and curcuminoid related compounds, respectively. For that purpose, 21 turmeric and 9 curry samples commercially available were analyzed in triplicate by a simple liquid–solid extraction procedure using dimethyl sulfoxide as extracting solvent. The obtained results demonstrate that the proposed profiles were excellent chemical descriptors for sample characterization and classification by principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA), achieving 100% classification rates. Curcuminoids and some specific phenolic acids such as trans-cinnamic, ferulic and sinapic acids, helped on the discrimination of turmeric samples; polyphenols, in general, were responsible for the curry sample distinction. Besides, the combination of both polyphenolic and curcuminoid profiles was necessary for the simultaneous characterization and classification of turmeric and curry samples. Discrimination among turmeric species such as Curcuma longa vs. Curcuma zedoaria, as well as among different Curcuma longa varieties (Alleppey, Madras and Erode) was also accomplished.

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Application of 1H-NMR based metabolite fingerprinting and chemometrics for authentication of Curcuma longa adulterated with C. heyneana

Cited by 21 publications

References 29 publications

The Application of Molecular Spectroscopy in Combination with Chemometrics for Halal Authentication Analysis: A Review

The Application of Molecular Spectroscopy in Combination with Chemometrics for Halal Authentication Analysis: A Review

The Authentication of Java Turmeric (Curcuma xanthorrhiza) Using Thin Layer Chromatography and 1H-NMR Based-Metabolite Fingerprinting Coupled with Multivariate Analysis

Characterization, Classification and Authentication of Turmeric and Curry Samples by Targeted LC-HRMS Polyphenolic and Curcuminoid Profiling and Chemometrics

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