Application and Development of Biocontrol Agents in China

Meng, Jiaxuan; Zhang, Xiuyu; Han, Xingshan; Fan, Bin

doi:10.3390/pathogens11101120

Cited by 10 publications

(7 citation statements)

References 15 publications

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“…The potential B. subtilis A9 is expected to be used to control the diseases of related edible fungi. The industrial production of biocontrol agents can be mainly grouped into two types: microbial pesticides and microbial fertilizers ( Meng et al, 2022 ). Our research should develop and innovate the optimal form of B. subtilis A9 biocontrol agents.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol potential of endophytic Bacillus subtilis A9 against rot disease of Morchella esculenta

Chen,

Zhang,

Chao

et al. 2024

Front. Microbiol.

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IntroductionMorchella esculenta is a popular edible fungus with high economic and nutritional value. However, the rot disease caused by Lecanicillium aphanocladii, pose a serious threat to the quality and yield of M. esculenta. Biological control is one of the effective ways to control fungal diseases.Methods and resultsIn this study, an effective endophytic B. subtilis A9 for the control of M. esculenta rot disease was screened, and its biocontrol mechanism was studied by transcriptome analysis. In total, 122 strains of endophytic bacteria from M. esculenta, of which the antagonistic effect of Bacillus subtilis A9 on L. aphanocladii G1 reached 72.2% in vitro tests. Biological characteristics and genomic features of B. subtilis A9 were analyzed, and key antibiotic gene clusters were detected. Scanning electron microscope (SEM) observation showed that B. subtilis A9 affected the mycelium and spores of L. aphanocladii G1. In field experiments, the biological control effect of B. subtilis A9 reached to 62.5%. Furthermore, the transcritome profiling provides evidence of B. subtilis A9 bicontrol at the molecular level. A total of 1,246 differentially expressed genes (DEGs) were identified between the treatment and control group. Gene Ontology (GO) enrichment analysis showed that a large number of DEGs were related to antioxidant activity related. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the main pathways were Nitrogen metabolism, Pentose Phosphate Pathway (PPP) and Mitogen-Activated Protein Kinases (MAPK) signal pathway. Among them, some important genes such as carbonic anhydrase CA (H6S33_007248), catalase CAT (H6S33_001409), tRNA dihydrouridine synthase DusB (H6S33_001297) and NAD(P)-binding protein NAD(P) BP (H6S33_000823) were found. Furthermore, B. subtilis A9 considerably enhanced the M. esculenta activity of Polyphenol oxidase (POD), Superoxide dismutase (SOD), Phenylal anineammonia lyase (PAL) and Catalase (CAT).ConclusionThis study presents the innovative utilization of B. subtilis A9, for effectively controlling M. esculenta rot disease. This will lay a foundation for biological control in Morchella, which may lead to the improvement of new biocontrol agents for production.

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Section: Discussionmentioning

confidence: 99%

Biocontrol potential of endophytic Bacillus subtilis A9 against rot disease of Morchella esculenta

Chen,

Zhang,

Chao

et al. 2024

Front. Microbiol.

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“…Timely identification of the pathogen responsible for C. sinensis leaf spot is essential. By combining morphological identification, molecular analysis, and phylogenetic research [36], A. alternata and A. koreana were identified as the causal agents of C. sinensis leaf spot in China. To the best of our knowledge, this is the first report of A. alternata and A. koreana causing leaf spot in C. sinensis.…”

Section: Discussionmentioning

confidence: 99%

Alternaria alternata and Alternaria koreana, the Causal Agents of Leaf Spot in Celtis sinensis and Their Sensitivity to Fungicides

Wang,

Zhang,

Wan

et al. 2023

Forests

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Celtis sinensis is a highly versatile species that is commonly cultivated in the southern regions of China. In June 2022, leaf spot disease was detected in C. sinensis in Nanjing, Jiangsu, China. Based on morphological characteristics, three isolates were determined to be of the Alternaria species. A phylogenetic analysis of combined ITS, GAPDH, TEF1-α, RPB2, and Alt a 1 sequences identified the three isolates we obtained as Alternaria alternata and A. koreana. We determined the pathogenicity of A. alternata and A. koreana in C. sinensis leaves using inoculation through in vivo experiments. Symptoms of inoculation onset in indoor pots were in accordance with those observed in the open country. A. alternata and A. koreana can grow at 15–35 °C, with the best growth occurring at 25 °C. The findings from fungicide susceptibility experiments demonstrated that A. alternata and A. koreana were the most sensitive to prochloraz, which could offer an effective approach for future prevention and control measures against A. alternata and A. koreana. This study provides the initial step toward further research on A. alternata and A. koreana as pathogens of C. sinensis and provides the stage for future control strategy development.

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“…In recent years, research on the application of microbial biocontrol agents has made substantial progress in several ways. In China, there has been a steady and very substantial increase in the development and application of the biological control of phytopathogens, as reported by Meng et al [ 12 ]. This is the result of China’s environmental protection policy in the last two decades, with increased attention on environmental protection, pollution control and ecological restoration.…”

Section: Applicationsmentioning

confidence: 99%