Applicability of Supercritical fluid chromatography–Mass spectrometry to metabolomics. II–Assessment of a comprehensive library of metabolites and evaluation of biological matrices

Losacco, Gioacchino Luca; Ismail, Omar H.; Pezzatti, Julian; González‐Ruiz, Víctor; Bernard, Julien; Rudaz, Serge; Veuthey, Jean‐Luc; Guillarme, Davy

doi:10.1016/j.chroma.2020.461021

Cited by 35 publications

(15 citation statements)

References 25 publications

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“…58,59 The improvement of instrument sensitivity and sample treatment and the combination of different analytical strategies become critical steps for greater metabolite coverage. 16 The most widely used analytical tools in metabolomics analysis are nuclear magnetic resonance spectroscopy (NMR) and the combination of mass spectrometry (MS) with separation techniques, 60,61 such as liquid chromatography (LC), gas chromatography (GC), 62,63 supercritical fluid chromatography (SCF) 64,65 or capillary zone electrophoresis (CZE). 66,67 The choice of the separation technique is based on (i) the sample matrix, (ii) metabolite abundances, (iii) physicochemical properties of the metabolites and (iv) the amount of sample.…”

Section: Data Acquisitionmentioning

confidence: 99%

Approaches in metabolomics for regulatory toxicology applications

Olesti

González‐Ruiz

Wilks

et al. 2021

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Section: Data Acquisitionmentioning

confidence: 99%

Approaches in metabolomics for regulatory toxicology applications

Olesti

González‐Ruiz

Wilks

et al. 2021

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“…The detection success was of 66%. However, poor performance was found for phosphorylated metabolites, and nucleotides [86]. Perrenoud et al.…”

Section: Applications Of Supercritical Fluid Chromatography Hyphenatementioning

confidence: 99%

Supercritical fluid chromatography hyphenated to mass spectrometry for metabolomics applications

Gordillo

2020

J of Separation Science

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While supercritical fluid chromatography was developed over 50 years ago, it is only over the past 15 to 20 years that it has become routinely utilized. Along with the commercialization of a new generation of instruments, during the last 20 years supercritical fluid chromatography has improved performance, reliability, and robustness. Supercritical fluid chromatography is fully compatible with mass spectrometric techniques. This review compiles the application of supercritical fluid chromatography separations coupled to mass spectrometry instrumentation for the exploration, profiling, and quantitation of metabolites during the last two decades. The selection of metabolites chosen for this article have direct applications in preclinical models of disease and clinical applications as potential biomarkers of disease including lipids, steroid hormones, bile acids, polar metabolites, peptides, and proteins.

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“…The advantages of SFC are that it is similar to normal phase techniques, and therefore no derivatisation is necessary. Furthermore, the use of CO 2 as a mobile phase reduces the environmental impact [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Comparative study for analysis of carbohydrates in biological samples

et al. 2021

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This work presents a comparative study for the analysis of carbohydrates for four common chromatographic methods, each coupled to mass spectrometry. Supercritical fluid chromatography (SFC), hydrophilic interaction liquid chromatography (HILIC), reversed-phase liquid chromatography (RP-LC) and gas chromatography (GC) with detection by triple quadrupole mass spectrometer (QqQ-MS) are compared. It is shown that gas chromatography and reversed-phase liquid chromatography, each after derivatisation, are superior to the other two methods in terms of separation performance. Furthermore, comparing the different working modes of the mass spectrometer, it can be determined that a targeted analysis, i.e. moving from full scan to single ion monitoring (SIM) and multiple reaction monitoring (MRM), results in an improvement in the sensitivity as well as the repeatability of the method, which has deficiencies especially in the analysis using HILIC. Overall, RP-LC–MS in MRM after derivatisation with 1-phenyl-3-methyl-5-pyrazolone (PMP) proved to be the most suitable method in terms of separation performance, sensitivity and repeatability for the analysis of monosaccharides. Detection limits in the nanomolar range were achieved, which corresponds to a mass concentration in the low µg/L range. The applicability of this method to different biological samples was investigated with various herbal liquors, pectins and a human glycoprotein. Graphical abstract

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Applicability of Supercritical fluid chromatography–Mass spectrometry to metabolomics. II–Assessment of a comprehensive library of metabolites and evaluation of biological matrices

Cited by 35 publications

References 25 publications

Approaches in metabolomics for regulatory toxicology applications

Approaches in metabolomics for regulatory toxicology applications

Supercritical fluid chromatography hyphenated to mass spectrometry for metabolomics applications

Comparative study for analysis of carbohydrates in biological samples

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