Apple scab control and activation of plant defence responses using potassium phosphite and chitosan

Felipini, Ricardo Barbosa; Boneti, José Itamar da Silva; Katsurayama, Yoshinori; Neto, A.C. Rocha; Veleirinho, Beatriz; Maraschin, Marcelo; Piero, Robson Marcelo Di

doi:10.1007/s10658-016-0881-2

Cited by 24 publications

(11 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The main focus of this study was to identify the mode of action of Phi against rust pathogens, which has remained unexplored. Priming of defense response genes by Phi in dicot species such as Arabidopsis, potato, lupin and apple has been studied, but not in depth (Eshraghi et al, 2011;Groves et al, 2015;Felipini et al, 2016). Our transcript profiling study focused on Phi-treated switchgrass allowed us to determine if the priming of defense response genes can also takes place in monocotyledons.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome‐based analyses of phosphite‐mediated suppression of rust pathogens Puccinia emaculata and Phakopsora pachyrhizi and functional characterization of selected fungal target genes

et al. 2018

View full text Add to dashboard Cite

Phosphite (Phi) is used commercially to manage diseases mainly caused by oomycetes, primarily due to its low cost compared with other fungicides and its persistent control of oomycetous pathogens. We explored the use of Phi in controlling the fungal pathogens Puccinia emaculata and Phakopsora pachyrhizi, the causal agents of switchgrass rust and Asian soybean rust, respectively. Phi primes host defenses and efficiently inhibits the growth of P. emaculata, P. pachyrhizi and several other fungal pathogens tested. To understand these Phi-mediated effects, a detailed molecular analysis was undertaken in both the host and the pathogen. Transcriptomic studies in switchgrass revealed that Phi activates plant defense signaling as early as 1 h after application by increasing the expression of several cytoplasmic and membrane receptor-like kinases and defense-related genes within 24 h of application. Unlike in oomycetes, RNA sequencing of P. emaculata and P. pachyrhizi did not exhibit Phi-mediated retardation of cell wall biosynthesis. The genes with reduced expression in either or both rust fungi belonged to functional categories such as ribosomal protein, actin, RNA-dependent RNA polymerase, and aldehyde dehydrogenase. A few P. emaculata genes that had reduced expression upon Phi treatment were further characterized. Application of double-stranded RNAs specific to P. emaculata genes encoding glutamate N-acetyltransferase and cystathionine gamma-synthase to switchgrass leaves resulted in reduced disease severity upon P. emaculata inoculation, suggesting their role in pathogen survival and/or pathogenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome‐based analyses of phosphite‐mediated suppression of rust pathogens Puccinia emaculata and Phakopsora pachyrhizi and functional characterization of selected fungal target genes

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Several studies have shown that KP can control some soil-borne pathogens, such as bacterial wilt of geraniums caused by R. solanacearum [22] and Fusarium wilt in Monterey pines [23]. Moreover, KP can trigger the expression of host defence genes to resist plant pathogen invasion [24,25] and directly inhibit the growth of pathogens, such as Phytophthora [26,27]. The metabolic processes of Phytophthora related to phosphorus absorption are likely affected by KP.…”

Section: Introductionmentioning

confidence: 99%

Potassium Phosphite Modulated the Soil Microbiome and Enriched the Antagonistic Bacteria Streptomyces Coelicoflavus and Paenibacillus Favisporus to Inhibit the Tomato Pathogen Ralstonia Solanacearum Synergistically

Su¹,

Mo²,

Sun³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Application of certain agricultural chemicals could modulate the soil microbiome and induce potential antagonistic microbes. However, the specific selective effects of agricultural chemicals on soil bacterial functions and their co-occurrences are not well understood, and no studies have verified that the enriched potential antagonistic microbes could enhance the antagonistic functions of the soil microbiome.Results: Here, the effects of potassium phosphite (KP), an environment-friendly agricultural chemical, on the soil bacterial composition, co-occurrences and antagonistic functions were determined, and the potential antagonistic bacteria against the tomato bacterial wilt pathogen Ralstonia solanacearum were isolated to test their functions and associations among these strains. Our results showed that application of KP enriched Bacillus, Paenibacillus and Streptomyces. The positive links among the OTUs belonging to these genera were increased, and positive associations between these OTUs and predicted genes related to antagonistic substance production were revealed. Two strains, Streptomyces coelicoflavus F13 and Paenibacillus favisporus Y7, were isolated, and they inhibited the growth of R. solanacearum. Genomic sequencing showed that both strains harboured streptomycin synthetic genes, and P. favisporus Y7 also contained surfactin synthetic gene cluster. Synergistic inhibition of R. solanacearum growth by P. favisporus Y7 and S. coelicoflavus F13 was observed in soil. Genome-scale metabolic modelling showed that dextrin and lactic acid were potential cross-feeding metabolites. In addition, the KP-modulated soil microbiome could suppress R. solanacearum growth. Conclusions: Our results highlight that a KP-modulated soil microbiome has considerable potential for biocontrol and indicate a new mechanism for the inhibition of R. solanacearum by KP-enriched soil bacteria.

show abstract

“…On the other hand, several other compounds have been studied as defense elicitors to improve disease management and resistance against apple scab. Examples include potassium phosphate, potassium carbonate, salicylic acid (SA) and its derivatives, silicon and chitosan [ 58 , 59 , 60 , 61 ]. However, despite their successful defense enhancement, various side/mixed effects, such as difficulties with formulation and environmental effects (e.g., ground water contamination), were observed [ 62 , 63 ].…”

Section: Introductionmentioning

confidence: 99%

Sweet Immunity: The Effect of Exogenous Fructans on the Susceptibility of Apple (Malus × domestica Borkh.) to Venturia inaequalis

Svara

Tarkowski

Rensburg

et al. 2020

IJMS

View full text Add to dashboard Cite

There is an urgent need for novel, efficient and environmentally friendly strategies to control apple scab (Venturia inaequalis), for the purpose of reducing overall pesticide use. Fructans are recently emerging as promising “priming” compounds, standing out for their safety and low production costs. The objective of this work was to test a fructan-triggered defense in the leaves of apple seedlings. It was demonstrated that exogenous leaf spraying can reduce the development of apple scab disease symptoms. When evaluated macroscopically and by V. inaequalis-specific qPCR, levan-treated leaves showed a significant reduction of sporulation and V. inaequalis DNA in comparison to mock- and inulin-treated leaves, comparable to the levels in fosetyl-aluminum-treated leaves. Furthermore, we observed a significant reduction of in vitro mycelial growth of V. inaequalis on plates supplemented with levans when compared to controls, indicating a direct inhibition of fungal growth. Variations in endogenous sugar contents in the leaves were followed during priming and subsequent infection, revealing complex dynamics as a function of time and leaf ontogeny. Our data are discussed in view of the present theories on sugar signaling and fructan-based immunity, identifying areas for future research and highlighting the potential use of fructans in apple scab management in orchards.

show abstract

Apple scab control and activation of plant defence responses using potassium phosphite and chitosan

Cited by 24 publications

References 48 publications

Transcriptome‐based analyses of phosphite‐mediated suppression of rust pathogens Puccinia emaculata and Phakopsora pachyrhizi and functional characterization of selected fungal target genes

Transcriptome‐based analyses of phosphite‐mediated suppression of rust pathogens Puccinia emaculata and Phakopsora pachyrhizi and functional characterization of selected fungal target genes

Potassium Phosphite Modulated the Soil Microbiome and Enriched the Antagonistic Bacteria Streptomyces Coelicoflavus and Paenibacillus Favisporus to Inhibit the Tomato Pathogen Ralstonia Solanacearum Synergistically

Sweet Immunity: The Effect of Exogenous Fructans on the Susceptibility of Apple (Malus × domestica Borkh.) to Venturia inaequalis

Contact Info

Product

Resources

About