Appearance of virus-like particles in Tritrichomonas foetus after drug treatment

Vancini, Ricardo; Benchimol, Marlene

doi:10.1016/j.tice.2005.03.009

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…Virus purification by ultracentrifugation was successfully performed with a positive control T. vaginalis isolate in our laboratory and has been shown to have a high success rate in detecting viruses in other organisms (Benchimol et al 2002a, b;Poulos et al 2006). Virus-like particles were detected in Tritrichomonas fetus by electron microscopy only after the trichomonads were treated with cytoskeleton-affecting chemicals including colchicine, vinblastine, taxol, nocodazole, and griseofulvin (Vancini and Benchimol 2005). Future attempts to detect viruses in T. gallinae should include such protocols.…”

Section: Discussionmentioning

confidence: 99%

Examination for double-stranded RNA viruses in Trichomonas gallinae and identification of a novel sequence of a Trichomonas vaginalis virus

et al. 2009

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To determine if double-stranded RNA (dsRNA) viruses exist and are potential virulence factors in Trichomonas gallinae, virus purification via ultracentrifugation was attempted for 12 T. gallinae isolates recovered from wild birds. Following purification, virus-like particles were not observed by transmission electron microscopy, nor were dsRNA segments visualized in agarose gels after electrophoresis of extracted RNA from any of the 12 T. gallinae isolates. However, virus particles and dsRNA segments were detected from a previously determined virus-infected T. vaginalis isolate as a control using identical purification procedures. Subsequent reverse transcription-polymerase chain reaction analysis of the dsRNA of the virus in this isolate revealed a novel sequence of the RNA-dependent RNA polymerase gene of T. vaginalis viruses.

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Section: Discussionmentioning

confidence: 99%

Examination for double-stranded RNA viruses in Trichomonas gallinae and identification of a novel sequence of a Trichomonas vaginalis virus

et al. 2009

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“…Here, we briefly review some T. foetus properties which are relevant to the development of new treatments directed towards bovine cattle suffering of trichomoniasis. Several drugs have been used to investigate the cytotoxic effect on T. foetus, such as colchicine, vinblastine, cytochalasin B [27], taxol, nocodazole, griseofulvin, lactacyst, and hydrogen peroxide [73]. Da Silva et al [71] analyzed the cytotoxic effect produced by PDT with aluminum phthalocyanine tetrasulfonated (AlPcS4) photosensitizer on T. foetus culture.…”

Section: Discussionmentioning

confidence: 99%

Basic biological aspects of Tritrichomonas foetus of re-levance to the treatment of bovines suffering of tricho-moniasis

Silva

Machado²,

Filho³

et al. 2011

OJAS

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Tritrichomonas foetus is a flagellate protozoan and the etiological agent of bovine genital tri-chomoniasis [1], which is an infectious vene- real disease. This parasite is usually found as- sociated with the mucosal surface of the uro- genital tract in females or the male preputial and penile membranes. In females, the clinical ma-nifestations may include abortion, with repe- tition of estrus at irregular intervals, vaginitis, cervicitis, endometritis, and pyometra. Parasi- tized males may have a discharge with small nodules in the preputial membrane. After that, the bulls have no clinical symptoms, and are thus an asymptomatic carrier that may spread the infection. Considering that a bull could cover up to twenty females [2], bovine genital trichomoniasis is a serious medical and veteri- nary problem, with economical repercussion for beef and milk production. As T. foetus is an amitochondrial and aerotolerant organism, en- ergy production under low O2 tension in the protozoan is done via hydrogenosome, which, as the name suggests, is the organelle where H2 is generated [3,4,5]. The molecular machinery of mitochondrial cell death is, therefore, absent in this parasite and the mechanism that activates of cell death program is not clear. This review seeks to understand the characteristics of the protozoan parasite T. foetus in order to propose new therapies for animals suffering from this infectious and contagious agent

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“…A recent study identified VLPs in isolates of Tritrichomonas foetus , a bovine pathogen, but required treatment with various cytoskeleton-affecting drugs to visualize the VLPs through electron microscopy [29]. On the other hand, a recent examination of isolates of Trichomonas gallinae , a significant fowl pathogen, revealed no evidence of viruses by electron microscopy or RNA purification [25], but did not include similar drug treatments as in the Tritrichomonas foetus study.…”

Section: Biological Propertiesmentioning

confidence: 99%

Trichomonasvirus: a new genus of protozoan viruses in the family Totiviridae

et al. 2010

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The family Totiviridae includes a number of viruses with monosegmented dsRNA genomes and isometric virions that infect either fungi or a number of medically important protozoan parasites such as Leishmania and Giardia. A new genus, Trichomonasvirus, was recently proposed for this family. Its name is based on the genus of its host organism, Trichomonas vaginalis, a protozoan parasite that colonizes the human genitourinary mucosa and is the most common non-viral sexually transmitted infection in the world. The type species of this new genus is Trichomonas vaginalis virus 1. Distinguishing characteristics of the new genus include infection of a human sexually transmitted parasite, stable mixed infection with more than one distinct Trichomonasvirus species, and sequence-based phylogenetic divergence that distinguishes it from all other family members.

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Appearance of virus-like particles in Tritrichomonas foetus after drug treatment

Cited by 6 publications

References 37 publications

Examination for double-stranded RNA viruses in Trichomonas gallinae and identification of a novel sequence of a Trichomonas vaginalis virus

Examination for double-stranded RNA viruses in Trichomonas gallinae and identification of a novel sequence of a Trichomonas vaginalis virus

Basic biological aspects of Tritrichomonas foetus of re-levance to the treatment of bovines suffering of tricho-moniasis

Trichomonasvirus: a new genus of protozoan viruses in the family Totiviridae

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