Appearance of US-like porcine epidemic diarrhoea virus (PEDV) strains before US outbreaks and genetic heterogeneity of PEDVs collected in Northern Vietnam during 2012-2015

Diep, Nguyen Van; Sueyoshi, Masuo; Izzati, Uda Zahli; Fuke, Naoyuki; Teh, Angeline Ping Ping; Lan, Nguyen Thi; Yamaguchi, Ryoji

doi:10.1111/tbed.12681

Cited by 29 publications

(36 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In May 2013, PED was first reported in Iowa State in the USA, and afterwards rapidly spread to the main pork product states within a year [9,10]. Thereafter, diarrhea outbreaks caused by variant PEDVs appeared in other Asian countries (Thailand, South Korea, Vietnam, and Philippines) [11][12][13][14], America (Canada, Mexico, etc) [15,16], and European countries (Austria, Ukraine, Belgium, Germany, etc) [17,18].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and phylogenetic analysis of porcine diarrhea associated viruses in southern China from 2012 to 2018

Zhang

Luo

et al. 2019

BMC Vet Res

View full text Add to dashboard Cite

BackgroundIn China, large-scale outbreaks of severe diarrhea caused by viruses have occurred in pigs since late 2010. To investigate the prevalence and genetic evolution of diarrhea-associated viruses responsible for the outbreaks, a total of 2987 field diarrheal samples collected from 168 pig farms in five provinces in Southern China during 2012–2018 were tested.ResultsPorcine epidemic diarrhea virus (PEDV) was most frequently detected virus with prevalence rates between 50.21 and 62.10% in samples, and 96.43% (162/168) in premises, respectively. Porcine deltacoronavirus (PDCoV) was the second prevalent virus with prevalence rates ranging from 19.62 to 29.19% in samples, and 70.24% (118/168) in premises, respectively. Both transmissible gastroenteritis virus (TGEV) and porcine rotavirus (PoRV) were detected at low prevalence rates of < 3% in samples and 10.12% in premises. In this study, we identified a newly emerged swine acute diarrhea syndrome coronavirus (SADS-CoV) in diarrheal samples of piglets from Fujian province in Southern China, and the prevalence rate of SADS-CoV was 10.29% (7/68). Co-infections of these diarrhea-associated viruses were common. The most frequent co-infection was PEDV with PDCoV, with an average detection rate of 12.72% (380/2987, ranging from 8.26–17.33%). Phylogenetic analysis revealed that PEDVs circulating in Southern China during the last 7 years were clustered with the variant strains of PEDV in genotype IIa. The most frequent mutations were present in the collagenase equivalent (COE) and epitope regions of the spike gene of the PEDVs currently circulating in the field. Genetic relationships of PDCoVs were closely related with Chinese strains, other than those present in the USA, South Korea, Thailand and Lao’s public.ConclusionsThe findings of this study indicated that variant PEDV, PDCoV, and SADS-CoV were leading etiologic agents of porcine diarrhea, and either mono-infections or co-infections of pathogenic enteric CoVs were common in pigs in Southern China during 2012–2018. Thus, significant attention should be paid in order to effectively prevent and control porcine viral diarrhea.

show abstract

Section: Introductionmentioning

confidence: 99%

Prevalence and phylogenetic analysis of porcine diarrhea associated viruses in southern China from 2012 to 2018

Zhang

Luo

et al. 2019

BMC Vet Res

View full text Add to dashboard Cite

show abstract

“…In Vietnam, PEDV was first observed in the southern provinces in 2009 (21). Published studies have demonstrated that PEDV is present in all major swine-producing regions in Vietnam (21)(22)(23)(24)(25)(26). A descriptive survey recently provided evidence that northern Vietnam is an endemic area for PEDV, with a high proportion of PED-positive farms (30.89%) (Mai et al, unpublished).…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Risk Factors for Porcine Epidemic Diarrhea Virus Infection in an Endemic Area of Vietnam

Ngan

Bui²,

Huynh

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

Porcine epidemic diarrhea virus (PEDV) causes enteritis, vomiting, watery diarrhea, and high mortality in suckling pigs, threatening the swine industry. Porcine epidemic diarrhea (PED) re-emerged globally in 2013 in many important swine-producing countries in Asia and the Americas. Several studies have identified the risk factors for the spread of PEDV in acute outbreaks. However, limited information is available on the risk factors for the transmission of PEDV in endemic regions. We hypothesized that poor biosecurity, location, and some social or cultural practices are the main risk factors for PEDV transmission in the Vietnamese pig population. The aim of this study was to evaluate the potential risk factors for the transmission of PEDV in an endemic area in Vietnam. In this case-control study, questionnaires containing 51 questions were completed for 92 PEDV-positive and 95 PEDV-negative farms. A logistic regression analysis was performed to assess the risk factors associated with PEDV infection. Province and the total number of pigs were included as random effects to determine their influence on the risk of PEDV infection. Twenty-nine variables of interest that have been associated with PEDV status were analyzed in a univariate analysis (P < 0.20), with backward stepwise selection. Only three of these 29 variables in four models remained significant PEDV risk factors in the final model: farrow-to-wean production type, distance from the farm to the slaughterhouse (< 1,000 m), and the presence of chickens on site (P < 0.05). This is the first study to identify the main risk factors for PEDV infection in an endemic area. Our findings suggest that hygiene measures should be strictly implemented on farms for the effective control and prevention of PEDV infection.

show abstract

“…Since October 2010, outbreaks of highly virulent PED caused by new variant strains have been reported almost all over the world [3][4][5] . Currently, although pigs have been immunized with commercial PED vaccines, which mostly include inactivated and attenuated vaccines, PED still frequently broke out and caused signi cant economic losses to the global swine industry [6,7] . Therefore, it is important to develop an effective vaccine for the prevention of PED.…”

Section: Introductionmentioning

confidence: 99%

Study of the immunogenicity of recombinant protein consisting of multiple epitopes of porcine epidemic diarrhea virus highly expressed in Sf9 cells

Liu¹,

Hu²,

Zhang³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Current inactivated and attenuated vaccines can not provide complete protection, resulting in the ongoing epidemic of porcine epidemic diarrhea virus (PEDV), which has caused significant economic losses in the swine industry worldwide. To develop an alternative efficient and economical vaccine, a fusion protein named S1CD containing multiple neutralizing epitopes of PEDV was designed and expressed in Sf9 cells. The expression yield was optimized by production of baculoviral constructs harboring multiple S1CD-expressing cassettes, and the immunogenicity of the purified protein was evaluated. Results The results showed that high-level expression of the S1CD fusion protein was achieved by multi-copy gene co-expression. The recombinant virus carrying three copies of S1CD gene under the control of p10 promoter showed the highest expression level, with a yield of 462 mg/L, which is about 2.6 or 3.5 fold that of the recombinant viruses carrying only one copy. BALB/c mice were subcutaneously immunized with purified S1CD protein three times at 2 week intervals. After immunization, S1CD protein could induce the mice produce high level of specific IgG antibodies and neutralizing antibodies. Conclusion Fusion S1CD protein consisting of multiple epitopes of porcine epidemic diarrhea virus was highly expressed by multi-copy gene co-expression. Moreover, it stimulated mice to produce high level of specific IgG antibodies and neutralizing antibodies. The S1CD protein expressed in this study could be used as a putative economic and efficient subunit vaccine against PEDV infection.

show abstract

Appearance of US-like porcine epidemic diarrhoea virus (PEDV) strains before US outbreaks and genetic heterogeneity of PEDVs collected in Northern Vietnam during 2012-2015

Cited by 29 publications

References 44 publications

Prevalence and phylogenetic analysis of porcine diarrhea associated viruses in southern China from 2012 to 2018

Prevalence and phylogenetic analysis of porcine diarrhea associated viruses in southern China from 2012 to 2018

Evaluating the Risk Factors for Porcine Epidemic Diarrhea Virus Infection in an Endemic Area of Vietnam

Study of the immunogenicity of recombinant protein consisting of multiple epitopes of porcine epidemic diarrhea virus highly expressed in Sf9 cells

Contact Info

Product

Resources

About