Appearance of myelin proteins in rat sciatic nerve during development

Wiggins, R. C.; Benjamins, Joyce A.; Morell, Pierre

doi:10.1016/0006-8993(75)90137-7

Cited by 106 publications

(58 citation statements)

References 22 publications

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“…As in a recent study with rat PNS tissue [Wiggins et at., 1975], the present study did not corroborate the appearance of Wolfgram proteins as a major contribution to nerve development [Wood, 1973]. In fact, it appears that Wolfgram protein which is prominent in CNS developing myelin Langes, 1976: Banik andSmith, 1977;Sheads et al, 1977] may not occur in the postnatally de veloping sciatic nerve.…”

Section: Discussionsupporting

confidence: 52%

“…Such an observation is consistent with those reported earlier [Slteads et al, 1977;Bernik and Smith, 1977] for CNS myelin. These changes in PNS myelin proteins have been lost in overall basic protein comparisons [Wood, 1973] and have been somewhat confused with histone con centrations [Wiggins et al, 1975] in previous studies.…”

Section: Discussionmentioning

confidence: 92%

“…However, with dif ferent techniques and in different species these high molecular weight proteins have pre viously been reported to increase with nerve development [Wiggins et a/., 1975], Preliminary studies [Luttges el al., in pre paration] using a procedure much like that reported by SJteads et al [1977] show that the heavy" versus 'light' distinction may be much less dramatic in the PNS compared to CNS myelin. One reason for this may be the over whelming contribution of the major myelin protein in the PNS, the glycoprotein, and the relatively smaller difference in molecular weights between the PNS glycoprotein and basic proteins as compared to the CNS Wolfgram proteins and basic proteins.…”

Section: Discussionmentioning

confidence: 99%

“…The results of these studies [Wood, 1973;Wiggins et al, 1975] were, however, limited by the available resolution of the polyacrylamide-gel electrophoresis (PAGE) systems employed, and by the subcellular fractiona tion procedures used. In the latter case, as sumed biophysical similarities justifying the use of identical fractionations in immature and mature tissues have encountered several empirical contradictions [Bcinik and Smith, 1977;Sheads et ah, 1977: Mat thieit et ah, 1974Konat and Clausen, 1978], For example, the differences, during development, between 'heavy' and 'light' myelin fractions provides a cautionary note in the use of 'standardized' fractionation procedures when comparing tis sues known to vary in other ways.…”

mentioning

confidence: 99%

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Changes in Sciatic Nerve Protein Composition during Postnatal Development of Mice

Groswald¹,

Luttges²

1979

Dev Neurosci

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Proteins from whole nerve were visualized on a SDS-PAGE system and compared to known proteins enriched in various subcellular tissues during nerve development in mice. Whole nerve samples avoid the a priori assumptions that the immature and mature biophysical characteristics of nerve tissues remain the same during nerve development. Total protein, nerve lengths and wet weights were recorded so that protein recovery and the relative amounts of specific protein species could be quantified. Electrophoretic analysis revealed many rapid alterations in the relative concentration of nerve proteins. Nuclear histones and high molecular weight proteins dominated the electrophoretic patterns of nerves during early development, but diminished rapidly in more mature nerves. The absolute amount of each protein species in a unit length of nerve yields a simplified view of protein alterations in the developing nerve. The importance of such characteristic changes for nerve development are discussed.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Changes in Sciatic Nerve Protein Composition during Postnatal Development of Mice

Groswald¹,

Luttges²

1979

Dev Neurosci

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“…However, the ratio between the small and the large compo nent of MBP increased in both control and mid mice. This developmental change was previously reported in the PNS [14,[33][34][35] and in the CNS [1-3, 6. 21,27].…”

Section: Discussionsupporting

confidence: 49%

Myelin Basic Protein Deficit in the PNS of <i>mld</i> Mutant Mice Recovers during Development

1983

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Myelination was studied between 15 and 135 days postnatally in peripheral nerves of myelin deficient (mld) mice and in unaffected littermates. The nerve weights were not affected by the mutation and showed a 4-fold increase during the developmental period studied. The amounts of myelin present in peripheral nerves, as shown by biochemical and morphological techniques, were slightly reduced in mld in comparison to control mice. In controls, the concentration of myelin doubled during the investigation period. The increase of myelin basic protein (MBP) in total nerve homogenate paralleled the deposition of myelin, but the MBP concentration remained constant in normal myelin. In contrast, in mld myelin MBP concentrations were extremely low until 60 days of age and increased thereafter to reach almost normal values at 135 days. Similarly, the amounts of myelin isolated at 85 and 135 days were normal. 2',3'-Cyclic nucleotide 3'-phosphodiesterase (CNP; EC 3.1.4.37), the myelin-specific enzyme, showed normal specific activities in mld nerves. In mld and control myelin, CNP-specific activities decreased during development suggesting a preferential localization of CNP in Schwann cell plasma membranes. In contrast to the central nervous system, other myelin proteins were not altered in mld peripheral nervous system (PNS) and the very low MBP content had no severe repercussions on the composition and structure of the myelin sheath. Furthermore, Schwann cells appeared normal in mld PNS. Nevertheless, more subtle alterations could be detected. Slightly decreased amounts of myelin were observed in young mld mice and preliminary results indicate discrete alterations of the myelin periodicity. After 60 days postnatally, when the repressed MBP synthesis was removed, MBP was incorporated into the myelin sheath and the MBP deficit was nearly corrected.

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Isolation of Myelin

Larocca

Norton

2006

CP Cell Biology

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The methods used to prepare myelin involve homogenization of the tissue in isotonic sucrose solution, followed by the isolation of myelin membranes by a series of steps that include density gradient centrifugation and differential centrifugation. Homogenization of nervous tissue in isotonic sucrose causes the myelin sheath to peel from the axon and form relatively large myelin vesicles. The large size of the myelin vesicles, together with the fact that myelin membrane has a lower density than other biological membranes, make differential centrifugation and density gradient centrifugation the main tools for the isolation of this membrane. Three protocols are outlined in this unit: isolation of a highly‐purified myelin fraction from the central nervous system (CNS); separation of a highly‐purified CNS myelin fraction into subfractions of different densities; and isolation of myelin from the peripheral nervous system (PNS).

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Appearance of myelin proteins in rat sciatic nerve during development

Cited by 106 publications

References 22 publications

Changes in Sciatic Nerve Protein Composition during Postnatal Development of Mice

Changes in Sciatic Nerve Protein Composition during Postnatal Development of Mice

Myelin Basic Protein Deficit in the PNS of <i>mld</i> Mutant Mice Recovers during Development

Isolation of Myelin

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