Changes in Sciatic Nerve Protein Composition during Postnatal Development of Mice

Groswald, Douglas E.; Luttges, Marvin W.

doi:10.1159/000112439

Cited by 12 publications

(8 citation statements)

References 15 publications

(35 reference statements)

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“…Our finding of a developmental change in proportions of the main basic proteins, with an increase of the smaller one (P,) relative to the larger (PI), corroborates the report of Groswald and Luttges (1979), who found a progressive accumulation of small basic protein in mouse sciatic nerve for 50 days postpartum. Basic proteins seem to change similarly in rats: Wiggins et al (1975) showed a relative increase in P, (designated P:!…”

Section: Discussionsupporting

confidence: 92%

“…The available evidence appears, however, to argue for a straightforward effect on myelin protein composition. This can be seen in the studies of Wiggens et al (1975) and Groswald and Luttges (1979), which have used whole nerves and which have shown variations in basic proteins similar to those we have obtained with isolated myelin. Our more recent studies (Greenfield et al, 1983) have also shown that the age/mutation-related changes in P, are found in analyses of whole nerves from YQ and old Qk and control mice.…”

Section: J Wezse Et Alsupporting

confidence: 79%

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Protein Composition of PNS Myelin: Developmental Comparison of Control and Quaking Mice

Weise

Greenfield

Brostoff

et al. 1983

Journal of Neurochemistry

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Protein compositions were determined for sciatic nerve myelin isolated from young and adult control and quaking (Qk) mice. Age-related changes in the relative amounts of large (Pl) and small (Pr) basic proteins were found. In control animals, the ratio Pr/Pl increased with age, a change similar to that observed for the large (Bl) and small (Bs) CNS myelin basic proteins of adult mice. Pr/Pl also increased with age in the Qk mouse sciatic nerve, but only to the point that the value in the adult Qk mouse was similar to that observed for young control animals, a situation reminiscent of the effect of the Qk mutation on CNS basic proteins. Thus, our data suggest that the Qk mutation has a similar effect on peripheral nervous system (PNS) and CNS basic proteins. Our findings are consistent with recent electrophoretic and immunochemical data showing that PNS and CNS myelin basic proteins in rodents are analogous, and they suggest that the genetic program controlling basic protein expression is common to oligodendroglia and Schwann cells.

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Section: Discussionsupporting

confidence: 92%

Section: J Wezse Et Alsupporting

confidence: 79%

Protein Composition of PNS Myelin: Developmental Comparison of Control and Quaking Mice

Weise

Greenfield

Brostoff

et al. 1983

Journal of Neurochemistry

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“…However, the ratio between the small and the large compo nent of MBP increased in both control and mid mice. This developmental change was previously reported in the PNS [14,[33][34][35] and in the CNS [1-3, 6. 21,27].…”

Section: Discussionsupporting

confidence: 84%

Myelin Basic Protein Deficit in the PNS of <i>mld</i> Mutant Mice Recovers during Development

1983

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Myelination was studied between 15 and 135 days postnatally in peripheral nerves of myelin deficient (mld) mice and in unaffected littermates. The nerve weights were not affected by the mutation and showed a 4-fold increase during the developmental period studied. The amounts of myelin present in peripheral nerves, as shown by biochemical and morphological techniques, were slightly reduced in mld in comparison to control mice. In controls, the concentration of myelin doubled during the investigation period. The increase of myelin basic protein (MBP) in total nerve homogenate paralleled the deposition of myelin, but the MBP concentration remained constant in normal myelin. In contrast, in mld myelin MBP concentrations were extremely low until 60 days of age and increased thereafter to reach almost normal values at 135 days. Similarly, the amounts of myelin isolated at 85 and 135 days were normal. 2',3'-Cyclic nucleotide 3'-phosphodiesterase (CNP; EC 3.1.4.37), the myelin-specific enzyme, showed normal specific activities in mld nerves. In mld and control myelin, CNP-specific activities decreased during development suggesting a preferential localization of CNP in Schwann cell plasma membranes. In contrast to the central nervous system, other myelin proteins were not altered in mld peripheral nervous system (PNS) and the very low MBP content had no severe repercussions on the composition and structure of the myelin sheath. Furthermore, Schwann cells appeared normal in mld PNS. Nevertheless, more subtle alterations could be detected. Slightly decreased amounts of myelin were observed in young mld mice and preliminary results indicate discrete alterations of the myelin periodicity. After 60 days postnatally, when the repressed MBP synthesis was removed, MBP was incorporated into the myelin sheath and the MBP deficit was nearly corrected.

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“…The increased proportion of the higher M, forms of MBP in quaking CNS is also apparent in the immunoblot of whole brain homogenate shown by Sorg et al (1986), although it is not mentioned in the results. During normal development of the mouse CNS and PNS, the proportion of the 14K MBP in myelin increases relative to the larger froms of MBP (Barbarese et al, 1978;Groswald and Luttges, 1979;Weise et al, 1983). Therefore, the greater proportions of the higher M, forms of MBP in quaking brain and nerve probably reflect the immaturity of the myelin in this mutant.…”

Section: Discussionmentioning

confidence: 90%

Myelin‐Associated Glycoprotein in the Central and Peripheral Nervous System of Quaking Mice

Ishii¹,

Johnson²,

Quarles³

1987

Journal of Neurochemistry

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The myelin-associated glycoprotein (MAG) was quantitated in the CNS and PNS of quaking mice and the levels compared to the levels of myelin basic protein (MBP) and 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) activity. In the brainstems of 36-day-old quaking mice, MBP, MAG, and CNPase were reduced to 12, 16, and 29% of control levels, respectively. In the sciatic nerves of the 36-day-old quaking mice, MBP and CNPase were 38 and 75% of control levels, respectively, whereas the concentration of MAG was unchanged or slightly increased. Similar quantitative results were obtained for the sciatic nerves and spinal roots of 7-month-old quaking mice. Immunoblots showed that the principal MAG band from the brainstems, sciatic nerves, and spinal roots of the quaking mice had a higher than normal apparent Mr. In addition, there was a minor component reacting with anti-MAG antiserum in the brainstems of the quaking mice that had a slightly lower Mr than control MAG and was not detected in the normal mice. The results for the quaking mice are compared with those from similar studies on other mutants with dysmyelination of the CNS and PNS.

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Changes in Sciatic Nerve Protein Composition during Postnatal Development of Mice

Cited by 12 publications

References 15 publications

Protein Composition of PNS Myelin: Developmental Comparison of Control and Quaking Mice

Protein Composition of PNS Myelin: Developmental Comparison of Control and Quaking Mice

Myelin Basic Protein Deficit in the PNS of <i>mld</i> Mutant Mice Recovers during Development

Myelin‐Associated Glycoprotein in the Central and Peripheral Nervous System of Quaking Mice

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