Apparent absorption of chlorophyll from spinach in an assay with dogs

Fernandes, Taís Motta; Gomes, Bárbara Bicalho; Lanfer-Marquez, Ursula Maria

doi:10.1016/j.ifset.2007.03.019

Cited by 45 publications

(27 citation statements)

References 26 publications

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“…To date, the reported chlorophyll metabolites isolated from animals have been demetalated (Egner et al, 2000;Fernandes et al, 2007;Scheie and Flaoyen, 2003). The acidic environment of the stomach is thought to bring about loss of magnesium from the chlorophyll (Ferruzzi and Blakeslee, 2007;Ma and Dolphin, 1999).…”

Section: Dietary Chlorophyll In Animalsmentioning

confidence: 99%

“…Given estimated concentrations of chlorophyll derivatives in the body (Egner et al, 2000;Fernandes et al, 2007;Scheie and Flaoyen, 2003) and the photon flux at 670 nm (Bachem and Reed, 1931;Barun et al, 2007;Bearden et al, 2001;Benaron et al, 1997;Chance et al, 1988;Eichler et al, 1977;Menaker et al, 1970;Vanbrunt et al, 1964;Wan et al, 1981;Zourabian et al, 2000), each chlorophyll metabolite would be expected to absorb only a few photons per second. As such, one might anticipate negligible amounts of additional energy.…”

Section: Atp Stores and Life Spanmentioning

confidence: 99%

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Light-harvesting chlorophyll pigments enable mammalian mitochondria to capture photonic energy and produce ATP

Zhang

Mihai

et al. 2013

Journal of Cell Science

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Sunlight is the most abundant energy source on this planet. However, the ability to convert sunlight into biological energy in the form of adenosine-59-triphosphate (ATP) is thought to be limited to chlorophyll-containing chloroplasts in photosynthetic organisms. Here we show that mammalian mitochondria can also capture light and synthesize ATP when mixed with a light-capturing metabolite of chlorophyll. The same metabolite fed to the worm Caenorhabditis elegans leads to increase in ATP synthesis upon light exposure, along with an increase in life span. We further demonstrate the same potential to convert light into energy exists in mammals, as chlorophyll metabolites accumulate in mice, rats and swine when fed a chlorophyll-rich diet. Results suggest chlorophyll type molecules modulate mitochondrial ATP by catalyzing the reduction of coenzyme Q, a slow step in mitochondrial ATP synthesis. We propose that through consumption of plant chlorophyll pigments, animals, too, are able to derive energy directly from sunlight.

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Section: Dietary Chlorophyll In Animalsmentioning

confidence: 99%

Section: Atp Stores and Life Spanmentioning

confidence: 99%

Light-harvesting chlorophyll pigments enable mammalian mitochondria to capture photonic energy and produce ATP

Zhang

Mihai

et al. 2013

Journal of Cell Science

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“…Although information on the absorption of natural chlorophyll derivates is limited, the results of Fernandes et al (2007) showed average apparent absorption of chlorophyll by 3.4 % after addition of spinach to the diet of dogs. In contrast to lipophilic chlorophyll derivates, uptake of watersoluble chlorophyllin derivates has been shown to be very efficient (from almost 45 to 60 % by intestinal cells in culture, Ferruzzi et al 2002b).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, more stable water-soluble derivates such as sodium copper chlorophyllin have been widely used in commercial products and investigated for a range of beneficial biological activities. Because of the limited intestinal absorptive capacity for chlorophyll (Fernandes et al 2007), the gastrointestinal tract is the site through which chlorophyll and its derivates deliver antioxidant and antimutagenic (Ibrahim et al 2007;Nagini et al 2015), antiapoptotic (Sharma et al 2007), immunomodulatory (Yin et al 2013) or deodorizing effects (Young and Beregi 1980). Chlorophyll has also been shown to be effective in wound healing (Brett 2005) and detoxification processes (Jubert et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Experimental application of Lactobacillus fermentum CCM 7421 in combination with chlorophyllin in dogs

Strompfová

Kubašová

Farbáková

et al. 2015

Appl Microbiol Biotechnol

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Chlorophyll belongs in a larger class of phytochemical plant pigments currently receiving more attention as a physiologically active dietary component. Although most research has focused on its biological activities such as its antioxidant, antimutagenic, anti-inflammatory or apoptotic effects in humans or rodents, there is limited knowledge at this time about the combinative possibilities of chlorophyll with probiotic bacteria. Our aim was to test the growth characteristics of canine-derived probiotic strain Lactobacillus fermentum CCM 7421 in the presence of different concentrations of chlorophyllin in vitro. Antimicrobial activity of chlorophyllin against canine indicator bacteria was also detected. In the in vivo study, chlorophyllin, L. fermentum CCM 7421 and the combination of both additives on faecal microbiota, faecal organic acid concentrations, haematological and immunological parameters in dogs were tested. Forty dogs were divided into 4 treatment groups; control (C); receiving chlorophyllin (60 mg/day/dog, CH group); L. fermentum CCM 7421 (10(8) CFU/day/dog, LF group); and both additives (CH + LF group), 10 dogs in each group. The experiment lasted for 28 days with a 14-day treatment period (sample collection at days 0, 7, 14 and 28). Results showed no growth inhibition of strain CCM 7421 by 0.05-0.25 % of chlorophyllin in broth after 24 h. Reduced growth of staphylococci, Listeria monocytogenes and Citrobacter freundii was observed at 1 % chlorophyllin (P < 0.05). In dogs, lower coliform bacteria numbers and higher concentration of propionic acid in faeces of the CH group during the treatment compared to baseline were detected (P < 0.01). Phagocytic activity of leukocytes was stimulated in all three treated groups of dogs (P < 0.05).

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“…Chlorophylls, the most abundant pigments in green plants, are gaining increasing importance in the human diet, not only as food colorants, but also as healthy food ingredients (Fernandes et al, 2007). Leafy vegetables are an important source of chlorophylls and other pigments (Znidarcic et al, 2011).…”

Section: Hyperspectral Imaging Applications In Leafy Vegetablesmentioning

confidence: 99%

Hyperspectral image applied to determine quality parameters in leafy vegetables

Lara¹,

Ángel²

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MASTER IN AGRO-ENGINEERING CO-SUPERVISOR: BELÉN DIEZMA IGLESIAS CO-SUPERVISOR: LOURDES LLEÓ GARCÍA MADRID, 2016He nacido hoy de madrugada, viví AGRADECIMIENTOS -ACKNOWLEDGEMENTSDesde que comencé este reto que ha sido la tesis doctoral, muchas cosas han cambiado. No ha sido un camino fácil. Muchas horas de esfuerzo y dedicación. Cambios que han puesto patas arriba mis metas y aspiraciones. Tener que reinventarse, volver a empezar, adaptarse a los cambios… Por suerte, no estoy solo en el camino. Muchas personas me lo han demostrado en este tiempo y me gustaría agradecérselo en estas líneas.Quisiera dar las gracias de forma muy especial a mis tutoras, Belén Diezma Iglesias y Lourdes Lleó García, por toda la ayuda prestada a lo largo de estos años. Por su supervisión, apoyo y empuje. Para mí han sido más que tutoras, amigas, y su empuje y ánimo han sido determinantes para que hoy pueda escribir este párrafo. Gracias de corazón y ojalá podamos seguir colaborando en el futuro.Special thanks also to Jean Michel Roger for his unconditional support and technical advice in this research, and also for his assistance during my stay in CEMAGREF, now IRSTEA, Montpellier (France). It is always a great pleasure working with him.A todas las personas que componen el grupo de investigación LPF-TAGRALIA. Me he sentido muy a gusto trabajando con vosotros y en los momentos que hemos compartido. Ojalá hubieran podido ser muchos más. En especial, quisiera agradecer su ayuda y apoyo a Margarita Ruiz-Altisent y a Pilar Barreiro Elorza, siempre dispuestas a compartir su conocimiento. Podéis contar conmigo cuando lo necesitéis.Al Ministerio de Ciencia e Innovación (MICINN) que ha financiado los trabajos de investigación llevados a cabo en esta tesis doctoral con los proyectos Multihort (AGL2008-05666-C02-01) y QualityLeaf (AGL2013-48529-R), junto con el proyecto Durasfrut II (AL11-P(I+D)-06) de la Universidad Politécnica de Madrid.A María Isabel Gil y Yolanda Garrido, del Centro de Edafología y Biología Aplicada del Segura, perteneciente al Consejo Superior de Investigaciones Científicas (CEBAS-CSIC), por su colaboración y ayuda en la generación de muestras y en la interpretación de resultados de una parte fundamental de este trabajo.A mis antiguos compañeros de la Escuela de Ingeniería Técnica Agrícola que se preocuparon por mí y me apoyaron en momentos muy difíciles. En especial, me gustaría dar las gracias a las profesoras Eva Cristina Correa Hernando y Virginia Díaz Barcos, que se volcaron en ayudarme en aquellos momentos y, gracias a ellas, pude encontrar pronto un nuevo reto profesional. Os estaré siempre agradecido.A mi familia. A mis padres y hermanos. A los amigos que han sido conscientes de este duro camino y se han preocupado por mí, dándome ánimos. A Devin Townsend por haber sido la banda sonora de esta Tesis Doctoral.A mi mujer, Laura, porque sus pasos han sido mi inspiración para decidirme a cumplir este reto. Todo llega y, no me cabe duda, nuestro trabajo también tendrá su recompensa.A Alicia, porque aún no lo sabe, pero...

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Apparent absorption of chlorophyll from spinach in an assay with dogs

Cited by 45 publications

References 26 publications

Light-harvesting chlorophyll pigments enable mammalian mitochondria to capture photonic energy and produce ATP

Light-harvesting chlorophyll pigments enable mammalian mitochondria to capture photonic energy and produce ATP

Experimental application of Lactobacillus fermentum CCM 7421 in combination with chlorophyllin in dogs

Hyperspectral image applied to determine quality parameters in leafy vegetables

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