Apoptotic Signaling Pathways in Uteri of Rats with Endometrial Hyperplasia Induced by Ovariectomy Combined with Estrogen

Abstract

Aims: To explore a new reliable method inducing an animal model similar to the morphology and apoptotic signaling pathways in endometrial hyperplasia patients. Methods: After the rats were ovariectomized, estradiol benzoate (60 µg/​100 g) was intramuscularly injected on alternate days for 4 weeks. The morphology in the uterus was observed under a light microscope and by electron microscopy. The expression levels of survivin/caspase-3 and Fas/FasL were checked by immunohistochemistry, Western blotting and real-time polymerase chain reaction. Results: After the models were induced, the edema and hypertrophy in uteri were observed 4 weeks later. The glands in the endometrium had increased, indented hyperplasia of glandular cells appeared, and a pseudo-stratified phenomenon occurred. Under a transmission electron microscope, free ribosomes had markedly increased and the nucleus was enlarged in the cytoplasm. Compared with the control group, the expression of survivin increased (p < 0.05) while that of caspase-3 and Fas/FasL declined (p < 0.05). Conclusions: In the rat model of endometrial hyperplasia induced by ovariectomy with pharmacological estrogen add-back treatment, survivin, caspase-3 and Fas/FasL signaling pathways play an important role in regulating the apoptosis of glandular cells in uteri.

The role of interleukin‐1b and its antagonist (diacerein) in estradiol benzoate‐induced endometrial hyperplasia and atypia in female rats

“…In our study, treatment with EB showed significant decrease in caspase‐3 immunoexpression compared to control group. These findings are in agreement with He et al and Guo et al .…”

“… Group I was received vehicle of DIA (1% carboxymethylcellulose) and intramuscular (im) olive oil three times per week for 4 weeks . Group II was treated with DIA (50 mg/kg/day orally) and im olive oil 3 times per week for 4 weeks . Group III was treated with vehicle of DIA (1% carboxymethylcellulose) and im injection of EB (60 μg/100 g) three times per week for 4 weeks . Group IV was treated with DIA (50 mg/kg/day orally) + im injection of EB (60 μg/100 g) three times per week for 4 weeks . …”

A Novel Preoperative Scoring System for Predicting Endometrial Cancer in Patients with Complex Atypical Endometrial Hyperplasia and Accuracy of Frozen Section Pathological Examination in This Context: A Multicenter Study

“…An association between obesity and EC is well recognized and is explained by unregulated estrogen expression [24,25]. An elevated BMI was significantly related to the presence of EC in ACH patients [26].…”

Protective Effects of Irbesartan, an Angiotensin Receptor Blocker with PPARγ Agonistic Activity, against Estradiol Benzoate-Induced Endometrial Hyperplasia and Atypia in Female Rats via Modulation of TNFα/Survivin Pathway

“…Survivin, a potent inhibitor of apoptosis, plays an essential role in EH [ 43 , 44 ]. He et al [ 44 ] reported an increase in survivin in EB-induced EH, which is in line with the present study results.…”

“…Survivin, a potent inhibitor of apoptosis, plays an essential role in EH [ 43 , 44 ]. He et al [ 44 ] reported an increase in survivin in EB-induced EH, which is in line with the present study results. On the other hand, survivin was significantly lower in the EH+IRB group compared to the EH group.…”