Apoptotic effects of insect tea in HepG2 human hepatoma cells

Suo, Huayi; Péng, Shigē; Wang, Cun; Peng, Deguang; Zhao, Xinquan

doi:10.1080/19476337.2015.1076521

Cited by 11 publications

(9 citation statements)

References 30 publications

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“…β-Actin was used as the internal reference gene, and the data were calculated by the 2 −∆∆CT method. 23 The primer sequences used are shown in Table 1.…”

Section: Rt-qpcr Analysismentioning

confidence: 99%

Lactobacillus plantarum KSFY06 Prevents Inflammatory Response and Oxidative Stress in Acute Liver Injury Induced by D-Gal/LPS in Mice

Sheng

Tan

et al. 2021

DDDT

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“…β-Actin was used as the internal reference gene, and the data were calculated by the 2 −∆∆CT method. 23 The primer sequences used are shown in Table 1.…”

Section: Rt-qpcr Analysismentioning

confidence: 99%

Lactobacillus plantarum KSFY06 Prevents Inflammatory Response and Oxidative Stress in Acute Liver Injury Induced by D-Gal/LPS in Mice

Sheng

Tan

et al. 2021

DDDT

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“…In a study on human tongue carcinoma cells, insect tea significantly induced apoptosis in cancer cells by upregulating BAX, CASP3, CASP9 and down regulating BCL2 171 . This was likewise the case for human liver cancer cells evaluated last year 172 . Anti-inflammatory activity was also evidenced through the down regulation of genes encoding nuclear factor kappa-light-chain-enhancer of activated B cells (NF κ B), inducible nitric oxide synthase (iNOS), and COX-2 171 .…”

Section: Lepidoptera (Butterflies and Moths)mentioning

confidence: 85%

Insects: an underrepresented resource for the discovery of biologically active natural products

Seabrooks¹,

Hu²

2017

Acta Pharmaceutica Sinica B

109

123

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Nature has been the source of life-changing and -saving medications for centuries. Aspirin, penicillin and morphine are prime examples of Nature׳s gifts to medicine. These discoveries catalyzed the field of natural product drug discovery which has mostly focused on plants. However, insects have more than twice the number of species and entomotherapy has been in practice for as long as and often in conjunction with medicinal plants and is an important alternative to modern medicine in many parts of the world. Herein, an overview of current traditional medicinal applications of insects and characterization of isolated biologically active molecules starting from approximately 2010 is presented. Insect natural products reviewed were isolated from ants, bees, wasps, beetles, cockroaches, termites, flies, true bugs, moths and more. Biological activities of these natural products from insects include antimicrobial, antifungal, antiviral, anticancer, antioxidant, anti-inflammatory and immunomodulatory effects.

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“…The cDNA samples for each gene were amplified three times in parallel, and the mean value of Ct was taken. The housekeeping gene GAPDH was used as an internal reference gene, and the levels of the related genes were calculated according to 2 −∆∆CT [19].…”

Section: Rt-qpcr Assaysmentioning

confidence: 99%

Evaluation of In Vitro Bio-Activities Effects of WST (Wushanshencha)

Liu

Zhang³

et al. 2019

Applied Sciences

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As a traditional Chinese drink, tea is favored for its rich flavor and its medicinal functionality. In this study, the in vitro bioactivities of Wushanshencha (WST; a local tea from Chongqing, China), which is processed mainly from the leaves of the wild Malus hupehensis (Pamp.) Rehd.). We assessed the scavenging capacity of tea extracts on 1, 1-diphenyl-2-picrylhydrazyl (DPPH); 2, 2′-azino-bis (3-ethylbenzthiazoline-6- sulphonic acid) diammonium salt (ABTS); and hydroxyl (OH) free radicals, and demonstrate the high antioxidant activity and dose-dependent relationship of these extracts. We also detail the anti-mutagenic effect of these tea extracts against the Salmonella typhimurium TA98 strain induced by the 2, 7-diaminofluorene (2, 7-AF) mutagen and the TA100 strain induced by the N-methyl-N′-nitro- N- nitrosoguanidine (MNNG) mutagen at concentrations of 1.25 and 2.50 mg/plate, respectively, with the high-dose groups showing better results. We investigated the anticancer mechanisms of WST extracts (40, 100, and 160 μg/mL) in HepG2 human hepatoma cells via 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay and quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR). The results showed that the proliferation of HepG2 cells was significantly inhibited in a dose-dependent manner by the tea extracts. Moreover, apoptosis in HepG2 cells was induced via upregulation of Caspase-3, Caspase-7, Caspase-8, Caspase-9, p21, p53, and Bax as well as downregulation of Bcl-2 apoptosis-associated factors, as assessed via mRNA expression levels after treating with WST extracts. The expression of inflammation-related factors, e.g., NF-κB, and Cox-2, was significantly downregulated by the WST extracts, demonstrating its inflammatory properties. Together, these observations indicated that WST extracts have anti-inflammatory and anti-cancer properties. In addition, high-performance liquid chromatography (HPLC) analysis showed that WST extracts contained chlorogenic acid, 4-hydroxycinnamic acid, isoquercitrin, taxifolin, quercitrin, rosmarinic acid, myricetin, baicalin, neosperidin dihydrochalcone, and quercetin. As such, WST appears to be an effectively functional drink, due to its rich functional components and anti-cancer activity.

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Apoptotic effects of insect tea in HepG2 human hepatoma cells

Cited by 11 publications

References 30 publications

Lactobacillus plantarum KSFY06 Prevents Inflammatory Response and Oxidative Stress in Acute Liver Injury Induced by D-Gal/LPS in Mice

Lactobacillus plantarum KSFY06 Prevents Inflammatory Response and Oxidative Stress in Acute Liver Injury Induced by D-Gal/LPS in Mice

Insects: an underrepresented resource for the discovery of biologically active natural products

Evaluation of In Vitro Bio-Activities Effects of WST (Wushanshencha)

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