Apoptotic effect of Epigallocatechin-3-gallate on the human gastric cancer cell line MKN45<i>via</i>activation of the mitochondrial pathway

Ran, Zhihua; Xu, Qi; Tong, Jinlu; Xiao, Shu–Dong

doi:10.3748/wjg.v13.i31.4255

Cited by 26 publications

(21 citation statements)

References 18 publications

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“…miR-16 may mediate the EGCG induction of apoptosis by targeting Bcl-2. EGCG was shown to induce apoptosis and also suppress Bcl-2 in different cell lines [19,20,32,33]. In addition to apoptosis induction, treatment of HepG2 cells with EGCG induced miR-16 and also down-regulated the expression of its target gene Bcl-2.…”

Section: Discussionmentioning

confidence: 98%

Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells

Tsang¹,

Kwok²

2010

The Journal of Nutritional Biochemistry

293

186

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Section: Discussionmentioning

confidence: 98%

Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells

Tsang¹,

Kwok²

2010

The Journal of Nutritional Biochemistry

293

186

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“…Several studies also revealed the effects of (−)-EGCG on gastric cancer [63,64]. The apoptotic effect of (−)-EGCG on the human gastric cancer cell line MKN45 was observed via activation of the mitochondrial pathway [63]. …”

Section: In Vitro and In Vivo Preclinical Studies On Tea Polyphenolsmentioning

confidence: 99%

EGCG, green tea polyphenols and their synthetic analogs and prodrugs for human cancer prevention and treatment

Chen

Wan

Yang

et al. 2011

Advances in Clinical Chemistry

185

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Cancer-preventive effects of tea polyphenols, especially epigallocatechin-3-gallate (EGCG), have been demonstrated by epidemiological, preclinical, and clinical studies. Green tea polyphenols such as EGCG have the potential to affect multiple biological pathways, including gene expression, growth factor-mediated pathways, the mitogen-activated protein kinase-dependent pathway, and the ubiquitin/proteasome degradation pathway. Therefore, identification of the molecular targets of EGCG should greatly facilitate a better understanding of the mechanisms underlying its anticancer and cancer-preventive activities. Performing structure–activity relationship (SAR) studies could also greatly enhance the discovery of novel tea polyphenol analogs as potential anticancer and cancer-preventive agents. In this chapter, we review the relevant literature as it relates to the effects of natural and synthetic green tea polyphenols and EGCG analogs on human cancer cells and their potential molecular targets as well as their antitumor effects. We also discuss the implications of green tea polyphenols in cancer prevention.

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“…EGCG, the major constituent in the tea, has been shown to have an inhibitory effect on gastrointestinal carcinogenesis in several studies 16–18 . In our previous study we demonstrated that EGCG could inhibit the growth of different digestive tumor cells 19 and EGCG can induce the mitochondrial‐dependent apoptotic pathway in the MKN45 cell line 20 . An experimental study suggests there is an inverse association between the risk of colon cancer and habitual tea consumption in the USA 21 .…”

Section: Discussionmentioning

confidence: 93%

“…[16][17][18] In our previous study we demonstrated that EGCG could inhibit the growth of different digestive tumor cells 19 and EGCG can induce the mitochondrial-dependent apoptotic pathway in the MKN45 cell line. 20 An experimental study suggests there is an inverse association between the risk of colon cancer and habitual tea consumption in the USA. 21 A population-based, case-control study of esophageal cancer in urban Shanghai suggests that green tea consumption has a protective effect.…”

Section: Discussionmentioning

confidence: 99%

Chemopreventive effect of epigallocatechin‐3‐gallate (EGCG) and folic acid on the N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG)‐induced gastrointestinal cancer in rat model

Yang

Liu

et al. 2011

J of Digest Diseases

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OBJECTIVE:To investigate the chemopreventive effect and mechanisms of epigallocatechin-3-gallate (EGCG) and folic acid on N-methyl-N′-nitro-Nnitrosoguanidine (MNNG)-induced gastrointestinal cancer in rats, and to investigate and compare the combinatorial effects of EGCG and folic acid on the chemoprevention of gastrointestinal carcinogenesis. METHODS:A total of 159 healthy male Wistar rats were randomly divided into seven groups to have the MNNG in drink (group M), MNNG in drink and EGCG in the feed (group ME), MNNG in drink and folic acid in the feed (group MF), MNNG in drink and EGCG + folic acid in the feed (group MEF), EGCG in the feed (group E), folic acid in the feed (group F) or normal feed (group C), respectively. At 44 weeks, all the rats were killed and assessed for the presence of gastrointestinal tumor. The occurrence of cancer was evaluated by histology. Ki-67 in cancerous tissues and in situ apoptosis were determined by immunohistochemical staining or terminal deoxyribonucleotide transferase-mediated nick-end labeling (TUNEL) assay, respectively. RESULTS:The experiment was completed in 157 rats (98.74%). As compared with group M, the tumor incidence of group MEF decreased significantly (P = 0.011). Ki-67 expression in cancerous tissues of group ME and MEF also decreased significantly (P = 0.038, P = 0.009), while apoptosis of group ME, MF and MEF increased significantly (P = 0.000, P = 0.003, P = 0.000).CONCLUSION: EGCG combined with folic acid has an obvious chemopreventive effect on gastrointestinal carcinogenesis induced by MNNG in rats.

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Apoptotic effect of Epigallocatechin-3-gallate on the human gastric cancer cell line MKN45viaactivation of the mitochondrial pathway

Abstract: These data support that EGCG can induce apoptosis of the human gastric cancer cell line MKN45, and the effect is in a time- and dose-dependent manner. The apoptotic pathway triggered by EGCG in MKN45 is mitochondrial-dependent.

Cited by 26 publications

References 18 publications

Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells

Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells

EGCG, green tea polyphenols and their synthetic analogs and prodrugs for human cancer prevention and treatment

Chemopreventive effect of epigallocatechin‐3‐gallate (EGCG) and folic acid on the N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG)‐induced gastrointestinal cancer in rat model

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