Apoptotic effect of demethoxyfumitremorgin C from marine fungus Aspergillus fumigatus on PC3 human prostate cancer cells

Kim, Young-Sang; Kim, Se-Kwon; Park, Sun Joo

doi:10.1016/j.cbi.2017.03.015

Cited by 20 publications

(16 citation statements)

References 14 publications

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“…fumigatus delays cell cycle progression and induces apoptotic cell death in PC3 advanced human prostate cancer cells. This result supports well our previous data, in which we indicated that DMFTC causes apoptosis as determined by Annexin V/PI staining and decreases mitochondrial membrane potential (Kim et al, 2017). We have also reported that DMFTC inhibits preferentially the proliferation of PC3 cells rather than other human normal and cancer cells lines, including MRC5, Hela, MCF-7, and HepG2 (Kim et al, 2017).…”

Section: Fisheries and Aquatic Sciencessupporting

confidence: 92%

“…To identify compounds with cytotoxic effect against human advanced prostate cancer cells from marine fungus A. fumigatus, DMFTC was purified from a pool of secondary metabolites. The chemical structure and molecular formula of DMFTC (C 21 H 23 N 3 O 2 ) were ascertained from 1 H, 13 C NMR, and LREI-MS spectroscopic data together with comparison to the data published previously (Cui et al, 1996(Cui et al, , 1997Kim et al, 2017). The compound was identified as DMFTC, as illustrated in Fig.…”

Section: A Fumigatusmentioning

confidence: 94%

“…Finally, we performed high-performance liquid chromatography (HPLC) (YMC ODS-A column; 250 × × 10 mm l.D, S-5 μm, 12 nm, methyl alcohol [MeOH]) to isolate DMFTC (final 7.2 mg). The structure and molecular formula of DMFTC, compared to previous data (Kim et al, 2017), were ascertained from 1 H, 13 C NMR (nuclear magnetic resonance), and low resolution electron impact mass spectrometry (LREI-MS) data. LREIMS m/z:…”

Section: Extraction and Isolationmentioning

confidence: 99%

“…However, there were few reports on the anticancer activity of compounds from A. fumigatus against cancer. Recently, we reported the isolation of demethoxyfumitremorgin C (DMFTC) from the ethyl acetate (EtOAc) extract of A. fumigatus strain MFS150 and the compound inhibits growth of human prostate cancer PC3 cells (Kim et al, 2017); however, the detailed data regarding to cell cycle arrest related to apoptosis has never been examined. In the present study, we revaluated the antiproliferative activity of DMFTC in human prostate cancer PC3 cells and investigated its functional mechanism on cell cycle progression.…”

Section: Introductionmentioning

confidence: 99%

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Aspergillus fumigatus-derived demethoxyfumitremorgin C inhibits proliferation of PC3 human prostate cancer cells through p53/p21-dependent G1 arrest and apoptosis induction

Kim¹,

Park²

2021

Fish Aquat Sci

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Human prostate cancer is the second most frequently diagnosed cancer worldwide, and its incidence rate continues to increase. Advanced prostate cancer is more difficult to treat than early forms due to its chemotherapy resistance. There is need for more effective agents that can inhibit the progression of advanced prostate cancer. Demethoxyfumitremorgin C (DMFTC) was isolated from the fermentation extract of the marine fungus Aspergillus fumigatus. Antiproliferative activity of DMFTC against human prostate cancer PC3 cells was examined through cell cycle analysis by flow cytometry, the fluorescent nuclear imaging analysis with propidium iodide (PI), and proteins expression related to cell cycle arrest and apoptosis were investigated via Western blotting. DMFTC inhibited PC3 cells growth through G1 phase cell cycle arrest and apoptosis induction. It activated the tumor suppressor p53 and the Cdk inhibitor p21, which regulate the cell progression into the G1 phase. Additionally, PI-positive late apoptotic non-viable cells were increased and the expression levels of the G1-positive downstream regulators cyclin D, cyclin E, Cdk2, and Cdk4 were decreased by DMFTC treatment. These results suggest that DMFTC induces G1 arrest and apoptosis induction through regulation of p53/p21-dependent cyclin-Cdk complexes, and it may be a useful therapeutic agent for the treatment of human advanced prostate cancer.

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Section: Fisheries and Aquatic Sciencessupporting

confidence: 92%

Section: A Fumigatusmentioning

confidence: 94%

Section: Extraction and Isolationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Aspergillus fumigatus-derived demethoxyfumitremorgin C inhibits proliferation of PC3 human prostate cancer cells through p53/p21-dependent G1 arrest and apoptosis induction

Kim¹,

Park²

2021

Fish Aquat Sci

Self Cite

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“…Demethoxyfumitremorgin C, a secondary metabolite of the marine fungus, Aspergillus fumigatus, had been reported to inhibited the cell viability on PC-3 cells [ 16 ].…”

Section: Bioactive Products With Potential For Prostate Cancer Trementioning

confidence: 99%

Investigation of the Anti-Prostate Cancer Properties of Marine-Derived Compounds

et al. 2018

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This review focuses on marine compounds with anti-prostate cancer properties. Marine species are unique and have great potential for the discovery of anticancer drugs. Marine sources are taxonomically diverse and include bacteria, cyanobacteria, fungi, algae, and mangroves. Marine-derived compounds, including nucleotides, amides, quinones, polyethers, and peptides are biologically active compounds isolated from marine organisms such as sponges, ascidians, gorgonians, soft corals, and bryozoans, including those mentioned above. Several compound classes such as macrolides and alkaloids include drugs with anti-cancer mechanisms, such as antioxidants, anti-angiogenics, antiproliferatives, and apoptosis-inducing drugs. Despite the diversity of marine species, most marine-derived bioactive compounds have not yet been evaluated. Our objective is to explore marine compounds to identify new treatment strategies for prostate cancer. This review discusses chemically and pharmacologically diverse marine natural compounds and their sources in the context of prostate cancer drug treatment.

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Medicinal Drug‐related Bioactive Agents from Marine Fungi

Saravanakumar

Rajendren²,

Kathiresan

et al. 2020

Encyclopedia of Marine Biotechnology

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Apoptotic effect of demethoxyfumitremorgin C from marine fungus Aspergillus fumigatus on PC3 human prostate cancer cells

Cited by 20 publications

References 14 publications

Aspergillus fumigatus-derived demethoxyfumitremorgin C inhibits proliferation of PC3 human prostate cancer cells through p53/p21-dependent G1 arrest and apoptosis induction

Aspergillus fumigatus-derived demethoxyfumitremorgin C inhibits proliferation of PC3 human prostate cancer cells through p53/p21-dependent G1 arrest and apoptosis induction

Investigation of the Anti-Prostate Cancer Properties of Marine-Derived Compounds

Medicinal Drug‐related Bioactive Agents from Marine Fungi

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