Apoptotic cell death in rat epididymis following epichlorohydrin treatment

Lee, I.-C.; Kim, K.-H.; Baek, Hyung-Seon; Moon, Changjong; Kim, S.-H.; Yun, Won‐Kee; Nam, Ki-Hoan; Kim, H.-C.; Kim, Jo-Chun

doi:10.1177/0960327112467042

Cited by 9 publications

(8 citation statements)

References 20 publications

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“…The mitogen‐activated protein kinases (MAPKs), as the serine/threonine protein kinases, are activated by different extracellular stimuli, such as cytokines, neurotransmitters, hormones, cell stress, and cell adhesion . The activated MAPKs can phosphorylate the nuclear transcription factor, then regulate the transcription of related genes, participating in tissue fibrosis . For mammals, members of the MAPKs superfamily mainly include the ERK, p38 MAPK, and c‐JUN, NH2‐terminal protein kinase (JNK) .…”

Section: Introductionmentioning

confidence: 99%

“…17 The activated MAPKs can phosphorylate the nuclear transcription factor, then regulate the transcription of related genes, participating in tissue fibrosis. 18 For mammals, members of the MAPKs superfamily mainly include the ERK, p38 MAPK, and c-JUN, NH2-terminal protein kinase (JNK). 19 Although study has shown that MAPK signaling pathway could be activated in the signal transduction of the TGF-β1-induced pulmonary fibrosis, 16 it still have differences between species and cell types.…”

Section: Introductionmentioning

confidence: 99%

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TGF‐β1 mediated MAPK signaling pathway promotes collagen formation induced by Nano NiO in A549 cells

Tian

Chang

Zhang

et al. 2019

Environmental Toxicology

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Nickel oxide nanoparticles (Nano NiO) could induce pulmonary fibrosis, however, the mechanisms are still unknown. The aim of the present study was to explore the roles of transforming growth factor‐β1 (TGF‐β1), mitogen‐activated protein kinase (MAPK) pathway and MMPs/TIMPs balance in Nano NiO‐induced pulmonary fibrosis. For that purpose, we first established Nano NiO‐induced human lung adenocarcinoma epithelial cells (A549 cells) model of collagen excessive formation through detecting the levels of hydroxyproline (Hyp) and type I collagen (Col‐I). Then the protein levels of TGF‐β1, MAPKs, and MMPs/TIMPs were assessed by Western blot. The results showed that Nano NiO resulted in the increased contents of Hyp, Col‐I, and TGF‐β1, the MAPK pathway activation and MMPs/TIMPs imbalance with a dose‐dependent manner. In addition, to investigate whether TGF‐β1 mediated MAPK signaling pathway, A549 cells were treated by 100 μg/mL Nano NiO combined with TGF‐β1, p38 MAPK, and ERK1/2 inhibitors (10 μM SB431542, 10 μM SB203580, and 10 μM U0126), respectively. We found that MAPK signal pathway was suppressed by TGF‐β1 inhibitor. Meanwhile, the increased contents of Hyp and Col‐I, and MMPs/TIMPs imbalance were alleviated by the p38 MAPK and ERK1/2 inhibitors, respectively. These findings indicated that the MAPK pathway and MMPs/TIMPs imbalance were involved in collagen excessive formation induced by Nano NiO.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

TGF‐β1 mediated MAPK signaling pathway promotes collagen formation induced by Nano NiO in A549 cells

Tian

Chang

Zhang

et al. 2019

Environmental Toxicology

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“…Afterward, different models of facilities were compared and selected. Previous studies also suggested that students’ independent inquiry around problems and reconstruction of knowledge would greatly contribute to cognition development and engineering practical ability. − …”

Section: Results and Discussionmentioning

confidence: 99%

“…Epichlorohydrin vapors are strongly irritating to the respiratory tract; inhalation at high concentrations causes central nervous system depression and can be fatal . Repeated and prolonged inhalation can cause lung, liver, and kidney injuries and even cause cancer . Direct skin or eyes contact with liquids may cause burns.…”

Section: Hazardsmentioning

confidence: 99%

From Lab Scale to Mass Production: A Project-Based Learning on the Preparation of (S)-Epichlorohydrin for Enhancing College Student Engineering Practical Abilities

et al. 2021

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Project-based learning (PJBL) is student-centered and teamwork educational activity that prepares students for the challenge of real-world problems. This kind of instructional method is highly recommended by the Accreditation Board for Engineering and Technology (ABET). This paper describes an authentic project that focuses on the preparation of (S)-epichlorohydrin from laboratory (lab) scale to mass production, which provides a situation for students to explore lab work as well as real industrial practice. During the different learning activities, clear learning objectives and guiding questions would be explicitly given to students to help them apply their knowledge and skills to the engineering project and complete the tasks within a given time. Upon the completion of the project, their written reports and team oral reports are used to evaluate their learning gains. The assessment results showed that most of the students attained good scores, and all teams reached outstanding level. Their engineering practical abilities including practical operation, problem-solving, systematic thinking, teamwork, and communication were significantly improved. This paper discusses how to cultivate engineering science talents at the college's comprehensive practicing course.

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“…Apoptosis is also known to be correlated with autoimmune diseases such as rheumatic arthritis [ 23 24 ], atherosclerosis, and cardiomyopathy. In particular, a study reported an increase in caspase-3 expression in epididymal tubules after the injection of epichlorohydrin—an antifertility agent that is directly toxic to the epididymis and inhibits sperm motility [ 25 ]. The expression of caspase-3 in the epididymal tubules of C57BL/6N mice, detected in the present study, was not observed in ICR or BALB/c nude mice or SD rats, which implies that it is characteristic to C57BL/6N mice.…”

Section: Discussionmentioning

confidence: 99%

Higher incidence of sperm granuloma in the epididymis of C57BL/6N mice

et al. 2018

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C57BL/6N mice are inbred strains widely used in biomedical research. Hence, a large amount of basic data has been accumulated. However, in the field of histopathology, spontaneous data for relatively younger mice that are used more frequently are not yet abundant, in contrast to data for older mice and their neoplastic lesions. To acquire the essential background data required by various research and toxicological assessments, 120 mice of the C57BL/6N strain (10 and 13 weeks of age) were collected from two institutions (From Korea and Japan) and subjected to histopathological analyses of the major organs (liver, spleen, kidney, thymus, heart, testis, epididymis). The results showed significantly higher incidence of sperm granulomas in the epididymides (10-56%) of these mice, compared with that in other strains or species of lab animals. Upon closer inspection, oligospermia/clear cell hyperplasia, cellular debris, and tubular vacuolation were also observed in the epididymides with sperm granulomas. Moreover, diseased organs were significantly heavier than healthy ones. Immunohistochemical staining showed a significant increase in the chromatic figures of cysteine-dependent aspartate-directed proteases-3 (caspase-3) and cleaved-poly(ADP-ribose) polymerase (c-PARP), and damages to the tubule due to spontaneous apoptosis, which may have led to the sperms leaking out of the tubule, causing the granuloma. To conclude, spontaneous sperm granuloma can occur in 10- and 13-week-old C57BL/6N mice and may thus affect the results of various studies using these mice. Therefore, sperm granuloma in epididymis needs to be carefully considered as an important factor when design the study using C57BL/6N.

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Apoptotic cell death in rat epididymis following epichlorohydrin treatment

Cited by 9 publications

References 20 publications

TGF‐β1 mediated MAPK signaling pathway promotes collagen formation induced by Nano NiO in A549 cells

TGF‐β1 mediated MAPK signaling pathway promotes collagen formation induced by Nano NiO in A549 cells

From Lab Scale to Mass Production: A Project-Based Learning on the Preparation of (S)-Epichlorohydrin for Enhancing College Student Engineering Practical Abilities

Higher incidence of sperm granuloma in the epididymis of C57BL/6N mice

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