Apoptotic and Vascular Changes in Trichinella Spiralis in Fected Mice After Parenteral Artemether Treatment

Ibrahim, Shereen Mahmoud; Sarhan, Mohamed; Farag, Tahani Ismail; Mohamed, Asmaa

doi:10.21608/jesp.2019.68282

Cited by 6 publications

(4 citation statements)

References 41 publications

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“…In our study, CD34 was markedly expressed in the small intestine, skeletal muscle and heart of infected non-treated mice. Ibrahim et al (2019) demonstrated the increased expression of CD34 in myoendothelial cells around encysted larvae in skeletal muscle of T. spiralis-infected mice, which was efficiently reduced by the administration of artemether. In the current study, the administration of curcumin lead to a significant decrease in CD34 expression in all tissue samples.…”

Section: Discussionmentioning

confidence: 96%

Investigation of the effect of curcumin on oxidative stress, local inflammatory response, COX-2 expression, and microvessel density in Trichinella spiralis induced enteritis, myositis and myocarditis in mice

et al. 2022

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Summary Background Curcumin exerts anti-oxidant and anti-inflammatory properties that have proven to be of value in the management of several parasitic infections. Objective Investigation of the value of curcumin in the management of trichinosis either alone or as an adjuvant to albendazole. Methods Animals received either curcumin 150 mg/kg, curcumin 300 mg/kg, albendazole 50 mg/ kg or combined curcumin 150mg/kg and albendazole 50 mg/kg and were compared with control infected and non-infected mice. Estimation of intestinal and muscular parasitic load and blood malondialdehyde level, in addition to the histopathological examination of small intestine, skeletal muscle tissue and heart was performed. Also, assessment of the local expression of cyclooxygenase-2 enzyme (COX-2) and CD34 in these samples was done by immunohistochemistry. Results Curcumin was found efficient in reducing parasitic load. It also lowered serum MDA level, local COX-2 and CD34 expression. An evident anti-inflammatory effect of curcumin was observed in intestinal, skeletal muscle and cardiac muscle histopathological sections. Conclusion The anti-inflammatory, anti-oxidant and anti-angiogenic effects of curcumin can help to improve trichinellosis-induced pathology. Curcumin can therefore be of value as an adjuvant therapy to conventional antiparasitic agents and can also produce promising results when used alone at higher doses.

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Section: Discussionmentioning

confidence: 96%

Investigation of the effect of curcumin on oxidative stress, local inflammatory response, COX-2 expression, and microvessel density in Trichinella spiralis induced enteritis, myositis and myocarditis in mice

et al. 2022

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“…T. spiralis up-regulates VEGF by stimulating thymosin β4 [ 17 , 57 ]. In Ibrahim et al [ 58 ] study on experimental muscular trichinosis, enhanced CD34 expression in myoendothelial cells was observed around the encysted larvae, which was subsequently reduced following administration of artemether. While in Fu et al [ 59 ] research, curcumin was reported to interfere with VEGF – VEGF receptor 2 signaling pathway resulting in reduced VEGF expression.…”

Section: Discussionmentioning

confidence: 99%

The neuroprotective potential of curcumin on T. Spiralis infected mice

Abdeltawab,

Abdel-Shafi,

Aboulhoda

et al. 2024

BMC Complement Med Ther

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Background Trichinella spiralis can affect the brain by inducing inflammatory and vascular changes. Drug management with the antiparasitic drug albendazole can be enhanced by natural compounds such as curcumin. The potential benefit of curcumin as an adjuvant to albendazole in the management of cerebral affection during experimental T. spiralis infection was evaluated. Animals received either curcumin 150 mg/Kg, albendazole 50 mg/Kg or a combination of both drugs. Animal groups receiving treatment were compared with infected and non-infected control groups. Blood levels of reduced glutathione (GSH) and dopamine were measured, and brain tissue expression of cyclooxygenase-2 enzyme (COX-2) and CD34 was assessed by immunohistochemistry. Results T. spiralis infection resulted in a state of oxidative stress, which was improved by albendazole and curcumin. Also, both drugs restored the peripheral dopamine level, which was decreased in infected non-treated mice. Curcumin was also found to be efficient in improving brain pathology and reducing local COX-2 and CD 34 expression. Conclusions Inflammatory and pathological changes during neurotrichinosis can be improved by the addition of curcumin to conventional anti-parasitic drugs.

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“…Also, weak expression of BcL-2 in early infection followed by strong positive staining for mitochondrial related genes as BAX (a pro-apoptotic member of BcL-2 family) in cellular infiltrates around capsule of encysted larvae. This was explained due to replacement of destructed mitochondria as a result of parasite invasion with new one (28)(29)(30) .…”

Section: Discussionmentioning

confidence: 99%

Amelioration of Myogenin, Bcl-2 expression and DNA damages in myocytes of Trichinella spiralis-infected mice after immunization with gamma radiation-attenuated larvae

Hafez

El-Kholy

2020

Int J Radiat Res

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Background:In trichinosis, the presence of muscle larvae inside skeletal striated muscle causes loss of identity and function of myotube through the releasing of myogenin and myosin. Considering the dominant role of vaccination, research on gamma radiation-attenuated vaccine has very promising value. The goal of this study was to determine the efficacy of vaccination with radiation-attenuated Trichinella spiralis larvae on the subsequent infectivity. This goal was to be achieved by expression of myogenin, BcL-2 and DNA damages of myocytes in experimental animals. Materials and methods: Mice were randomly assigned to serve as normal control, infected control, vaccinated unchallenged and vaccinated challenged groups. Histopathological and immunohistochemical assessments of skeletal muscle tissues of diaphragm were conducted using different stains. Also, DNA damages of myocytes by comet assay were evaluated. Results: An improvement of histopathological changes and DNA damages of myocytes with significant decreased expression of myogenin and BcL-2 in vaccinated challenged group was detected. Conclusion: Vaccination with gamma radiation-attenuated larvae exerted protective effect against challenged infection. This may pave the way to new protective modality in trichinosis control.

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Apoptotic and Vascular Changes in Trichinella Spiralis in Fected Mice After Parenteral Artemether Treatment

Cited by 6 publications

References 41 publications

Investigation of the effect of curcumin on oxidative stress, local inflammatory response, COX-2 expression, and microvessel density in Trichinella spiralis induced enteritis, myositis and myocarditis in mice

Investigation of the effect of curcumin on oxidative stress, local inflammatory response, COX-2 expression, and microvessel density in Trichinella spiralis induced enteritis, myositis and myocarditis in mice

The neuroprotective potential of curcumin on T. Spiralis infected mice

Amelioration of Myogenin, Bcl-2 expression and DNA damages in myocytes of Trichinella spiralis-infected mice after immunization with gamma radiation-attenuated larvae

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