Apoptosis of Peripheral CD4<sup>+</sup>T-Lymphocytes in End-Stage Renal Disease Patients Under Hemodialysis and rhEPO Therapies

Borges, Ana Pinto; Borges, Marisa; Fernandes, João; Nascimento, Henrique; Faria, Maria do Sameiro; Miranda, Vasco; Reis, Flávio; Belo, Luı́s; Costa, Elı́sio; Santos-Silva, Alice

doi:10.3109/0886022x.2011.553300

Cited by 30 publications

(39 citation statements)

References 21 publications

(25 reference statements)

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“…Our results are consistent with that of previous studies, 2,3,10,12,13 that reported that ESRD patients have a significant decrease in T-lymphocytes populations.…”

Section: Discussionsupporting

confidence: 83%

“…Furthermore, in vivo, uremic T lymphocytes expressed Fas with a higher intensity than control T cells, suggesting that uremic T cells undergo apoptosis. Borges et al 2 found that ESRD patients showed a decrease in the Mann-Whitney test. Data presented as means ± SD; p 0.05 is significant; NS, non-significant.…”

Section: Discussionmentioning

confidence: 99%

“…It may be due to causes related both to the disease (e.g., uremic toxins, increased oxidative stress, anemia, and malnutrition) and to the renal replacement therapy. [2][3][4][5] In most studies that had been conducted in dialysis patients, it is not clear whether these complications are due to the dialysis procedures or uremia per se. Few studies were conducted on pre-dialyzed ESRD patients had shown increases in pro-inflammatory cytokines and changes in leukocyte activation, indicating that dialysis alone is not responsible for alterations in the inflammatory state.…”

Section: Introductionmentioning

confidence: 99%

“…6,7 A number of immunological theories and studies on lymphocyte subpopulation and lymphocyte functions have been carried out on adult patients with ESRD to identify any abnormal T and B cell clones and to investigate its possible role in immune deficiencies. 2,4,5 A small number of studies in children with ESRD suggested an altered inflammatory and immunological state. 3,8 Since apoptosis plays an important role in lymphocyte homeostasis, the aim of this study was an analysis of lymphocyte subpopulation and detection of apoptosis in the peripheral B and T lymphocytes through flow cytometry in a cohort of children with ESRD on maintenance hemodialysis (HD).…”

Section: Introductionmentioning

confidence: 99%

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Lymphocyte populations and apoptosis of peripheral blood B and T lymphocytes in children with end stage renal disease

et al. 2014

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Background: End stage renal disease (ESRD) is a worldwide devastating health problem due to its increased prevalence in the population and high association with several pathologic conditions including immunodeficiency, which makes a significant contribution to morbidity and mortality. Aim: The present study aimed at analysis of T and B lymphocyte subpopulation and the detection of flowcytometric apoptosis markers on peripheral B and T lymphocytes in a cohort of children with ESRD. Subjects and methods: A case-control study was conducted on 28 children with ESRD. In addition, 30 age and sex matched healthy children were included as a control group. We used Annexin V-FITC binding assay as a sensitive probe for identifying cells undergoing apoptosis. Results: Circulating neutrophils, T and B lymphocytes were lower in patient group. In addition, apoptotic B and T lymphocytes occurred more frequently in children with ESRD than in the control group. Conclusion: Our finding of low numbers of circulating neutrophils, T and B lymphocytes, and increased portion of apoptotic B and T lymphocytes in children with ESRD, may emphasize the fact that these derangements are the main mechanisms responsible for the impairment of the immune system in ESRD children, also it adds to the fact that both cellular and humoral immunity affected in ESRD children. Finally, uremia and increased peripheral lymphocyte apoptosis were the major causes of lymphocyte populations' depletion in our ESRD patients.

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“…Our results are consistent with that of previous studies, 2,3,10,12,13 that reported that ESRD patients have a significant decrease in T-lymphocytes populations.…”

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lymphocyte populations and apoptosis of peripheral blood B and T lymphocytes in children with end stage renal disease

et al. 2014

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“…12 Several studies indicated a reduction of peripheral blood T and B lymphocytes in patients with ESRD; however, the result remains controversial. [13][14][15][16][17][18][19] Of note, most of these studies on immune dysfunction have been restricted to ESRD patients. This is necessarily inadequate for the full understanding of impacts of CKD on immune system.…”

Section: Introductionmentioning

confidence: 99%

Lymphocyte depletion and subset alteration correlate to renal function in chronic kidney disease patients

et al. 2015

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Effect of Erythropoietin on Lymphocytes Apoptosis in Experimental Chronic Renal Failure

2015

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Recombinant human erythropoietin was injected intraperitoneally in a total dose of 900 U/kg to rats with experimental chronic renal failure. Suspension of lymphocytes from animals with chronic renal failure was used in vitro, erythropoietin was used in concentrations of 30, 15, 7.5, 3.75, and 1.88 U/liter. Intact cells (Annexin-5-FITC(-)/7-AAD(-)), cells with early signs of apoptosis (Annexin-5-FITC(+)/7-AAD(-)), cells with late signs of apoptosis and partially necrotic cells (Annexin-5-FITC(+)/7-AAD(+)), as well as cells with early signs of necrosis (Annexin-5-FITC(-)/7-AAD(+)) were differentiated by fl ow cytometry. It was found that the number of peripheral blood lymphocytes with early and late signs of apoptosis and necrosis increased in chronic renal failure. Erythropoietin at a total dose of 900 U/kg reduced the number of blood lymphocytes with signs of apoptosis and necrosis and thus elevated the number of intact lymphocytes. Erythropoietin in concentrations ranging from 1.88 to 30.0 U/liter dose dependently lowered the number of lymphocytes with early signs of apoptosis and the number of lymphocytes with the signs of late apoptosis and necrosis in vitro.

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Apoptosis of Peripheral CD4⁺T-Lymphocytes in End-Stage Renal Disease Patients Under Hemodialysis and rhEPO Therapies

Cited by 30 publications

References 21 publications

Lymphocyte populations and apoptosis of peripheral blood B and T lymphocytes in children with end stage renal disease

Lymphocyte populations and apoptosis of peripheral blood B and T lymphocytes in children with end stage renal disease

Lymphocyte depletion and subset alteration correlate to renal function in chronic kidney disease patients

Effect of Erythropoietin on Lymphocytes Apoptosis in Experimental Chronic Renal Failure

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Apoptosis of Peripheral CD4+T-Lymphocytes in End-Stage Renal Disease Patients Under Hemodialysis and rhEPO Therapies

Cited by 30 publications

References 21 publications

Lymphocyte populations and apoptosis of peripheral blood B and T lymphocytes in children with end stage renal disease

Lymphocyte populations and apoptosis of peripheral blood B and T lymphocytes in children with end stage renal disease

Lymphocyte depletion and subset alteration correlate to renal function in chronic kidney disease patients

Effect of Erythropoietin on Lymphocytes Apoptosis in Experimental Chronic Renal Failure

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Apoptosis of Peripheral CD4⁺T-Lymphocytes in End-Stage Renal Disease Patients Under Hemodialysis and rhEPO Therapies