Apoptosis of Mo7e Leukemia Cells Is Associated with the Cleavage of Bcl-2 into a Shortened Fragment That Is Not Functional for Heterodimerization with Bcl-2 and Bax

Lin, Hong; Zhang, Xuemin; Chen, Catheryne; Chen, Ben D.

doi:10.1006/excr.2000.5036

Cited by 10 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cleavage of Bcl-2 [66] is a well known factor that may amplify apoptosis [69]. Bax can then undergo conformational changes and translocation to the mitochondria [70]. In addition, cleaved Bcl-2, lacking its N-terminal peptide, may acquire itself a pro-apoptotic function most likely by adapting a Bax-like conformation [71,72].…”

Section: Discussionmentioning

confidence: 99%

Cell cycle arrest in early mitosis and induction of caspase-dependent apoptosis in U937 cells by diallyltetrasulfide (Al2S4)

et al. 2009

View full text Add to dashboard Cite

Naturally occurring organic sulfur compounds (OSCs), such as linear allylsulfides from Allium species, are attracting attention in cancer research, since several OSCs were shown to act beneficially both in chemoprevention and in chemotherapy, while hardly exerting any harmful side effects. Hence, we investigated the possible role of different OSCs in the treatment of leukemia. Thereby, we found that the compounds tested in this study induced apoptosis in U937 cells, with an efficiency depending on the number of sulfides, and selected the most promising candidate, diallyltetrasulfide (Al2S4), for detailed mechanistic studies. Here we show that Al2S4 induced an accumulation of cells in early mitosis (G2/M phase), followed by the activation of caspase-dependent apoptosis. The compound counteracted different anti-apoptotic Bcl-2 family members (Bcl-xL, phospho-Bad and Bcl-2), promoted activation of Bax and Bak and induced the release of cytochrome c into the cytoplasm. Treatment by Al2S4 let to the identification of early apoptotic events including Bcl-xL degradation, Bak activation and release of cytochrome c followed by late events including Bcl-2 proteolysis, Bax activation, Bad dephosphorylation, caspase activation, nuclear fragmentation and phosphatidylserine exposure.

show abstract

Section: Discussionmentioning

confidence: 99%

Cell cycle arrest in early mitosis and induction of caspase-dependent apoptosis in U937 cells by diallyltetrasulfide (Al2S4)

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Cellular stress induced proapoptotic Bcl-2 family members to translocate from the cytosol to the mitochondria, where they induced the release of cytochrome c, while the antiapoptotic Bcl-2 proteins worked to prevent cytochrome c release from mitochondria, and thereby preserved cell survival (21). Especially, unlike Bcl-2, the cleaved Bcl-2 fragment was no longer functional for dimerization with either Bcl-2 or Bax (23).…”

Section: Discussionmentioning

confidence: 99%

Modulations of the Bcl-2/Bax Family Were Involved in the Chemopreventive Effects of Licorice Root (Glycyrrhiza uralensis Fisch) in MCF-7 Human Breast Cancer Cell

Hee-Do

Ahn

et al. 2004

J. Agric. Food Chem.

View full text Add to dashboard Cite

Recently, cancer chemoprevention with strategies using foods and medicinal herbs has been regarded as one of the most visible fields for cancer control. Genistein in soy, American ginseng, and resveratrol are well-known to have antiproliferative properties in human breast cancer. Licorice root is a botanical, a shrub native to southern Europe and Asia, which primarily has desirable qualities in sweetening and herbal medicine. In this study, licorice (Glycyrrhiza uralensis Fisch) root also inhibits cell proliferation in human breast cancer cell. The cell proliferation study demonstrated that licorice root reduced the proliferation of MCF-7 cells in a dose- and time-dependent manner. The extracts were fractionated in CHCl(3), EtOAc, C(6)H(14), and CH(3)OH-H(2)O (70:30), and these extracts of licorice root (50 microg/mL) induced DNA fragmentation demonstrated by Hoechst 33258 staining. Apoptosis also determined the sub-G1 accumulation by flow cytometry analysis. These results were consistent with specific cleavage of PARP and antiapoptotic protein Bcl-2 and up-regulation of proapoptotic protein Bax demonstrated by Western blotting. Our findings suggest that licorice root may have chemopreventive effects against human breast cancer through the modulation of the expression of the Bcl-2/Bax family of apoptotic regulatory factors.

show abstract

“…The latter phenomenon was at least partially due to the caspase 3-dependent cleavage of Bcl-2 because of the appearance of characteristic 22-kd Bcl-2 fragment, 61 which can further accelerate apoptosis. 62,63 In addition to its antiapoptotic effect, BCR/ABL also regulates the cell cycle. 57 Pim-1 may contribute to this process through phosphorylation and activation of Cdc25A.…”

Section: Discussionmentioning

confidence: 99%

Complementary functions of the antiapoptotic protein A1 and serine/threonine kinase pim-1 in the BCR/ABL-mediated leukemogenesis

Nieborowska-Skorska

Hoser

Kossev

et al. 2002

Blood

108

View full text Add to dashboard Cite

Apoptosis of Mo7e Leukemia Cells Is Associated with the Cleavage of Bcl-2 into a Shortened Fragment That Is Not Functional for Heterodimerization with Bcl-2 and Bax

Cited by 10 publications

References 23 publications

Cell cycle arrest in early mitosis and induction of caspase-dependent apoptosis in U937 cells by diallyltetrasulfide (Al2S4)

Cell cycle arrest in early mitosis and induction of caspase-dependent apoptosis in U937 cells by diallyltetrasulfide (Al2S4)

Modulations of the Bcl-2/Bax Family Were Involved in the Chemopreventive Effects of Licorice Root (Glycyrrhiza uralensis Fisch) in MCF-7 Human Breast Cancer Cell

Complementary functions of the antiapoptotic protein A1 and serine/threonine kinase pim-1 in the BCR/ABL-mediated leukemogenesis

Contact Info

Product

Resources

About