Apoptosis in cryopreserved eukaryotic cells

Savitskaya, M. A.; Онищенко, Г. Е.

doi:10.1134/s0006297916050023

Cited by 13 publications

(5 citation statements)

References 54 publications

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“…However, the air density in the stratosphere is about 1000 times lower than on sea level and because of that the temperature exchange between the biological samples and air was limited, resulting in the relative temperature stability of cells. Along with extensive structural damage of the cell, freezing and thawing cycles are associated with oxidative stress and consequently mitochondrial disturbance, cell membrane permeabilization or DNA damage (71), finally leading to death either on apoptotic or necrotic pathway (72,73), which was reflected in our research. The biological samples after the balloon flight were characterized with increase of ROS generation and percentage ratio of dead cells; however, treatment with selected antioxidants limited these processes.…”

Section: Discussionmentioning

confidence: 56%

The cytoprotective role of antioxidants in mammalian cells under rapidly varying temperature, pressure and UV conditions during stratospheric balloon campaign

Górska

Rozborska

Bartosik

et al. 2019

Preprint

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1.AbstractCurrently ongoing age of the dynamic development of the space industry brings the mankind closer to the routine manned space flights and space tourism. That progress leads to a demand for intensive astrobiological research aimed at improving strategies of the pharmacological protection of the human cells against extreme conditions. Although routine research in space remain out of our reach, it is worth noticing that unique severe environment of the Earth’s stratosphere have been found to mimic subcosmic conditions, giving rise to the opportunity for use of stratospheric surface as a research model for the astrobiological studies. Our study included launching balloon into the stratosphere containing the human normal and cancer cells treated with various compounds to examine whether these medicines are capable to protect the cells against the stress caused by rapidly varying temperature, pressure and radiation, especially UV. Due to oxidative stress caused by irradiation and temperature shock, we used natural compounds which display antioxidant properties, namely catechin isolated from green tea, honokiol derived from magnolia, curcumin from turmeric and cinnamon extract. “After-flight” laboratory tests displayed the most active antioxidants as potential agents which can minimize harmful impact of extreme conditions to the human cells.

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Section: Discussionmentioning

confidence: 56%

The cytoprotective role of antioxidants in mammalian cells under rapidly varying temperature, pressure and UV conditions during stratospheric balloon campaign

Górska

Rozborska

Bartosik

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…However, the air density in the stratosphere is ∼1,000 times lower than on sea level, and because of that, the temperature exchange between the biological samples and air was limited, resulting in the relative temperature stability of cells. Along with extensive structural damage of the cell, freezing and thawing cycles are associated with oxidative stress and consequently mitochondrial disturbance, cell membrane permeabilization, or DNA damage (Karimi-busheri et al, 2016), finally leading to death, triggering either apoptotic or necrotic pathway (Baust et al, 2009; Savitskaya and Onishchenko, 2016), which was reflected in our research.…”

Section: Discussionmentioning

confidence: 58%

The Cytoprotective Role of Antioxidants in Mammalian Cells Under Rapidly Varying UV Conditions During Stratospheric Balloon Campaign

et al. 2019

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The current age of dynamic development of the space industry brings the mankind closer to routine manned space flights and space tourism. This progress leads to a demand for intensive astrobiological research aimed at improving strategies of the pharmacological protection of the human cells against extreme conditions. Although routine research in space remains out of our reach, it is worth noticing that the unique severe environment of the Earth’s stratosphere has been found to mimic subcosmic conditions, giving rise to the opportunity to use the stratospheric surface as a research model for the astrobiological studies. Our study included launching into the stratosphere a balloon containing mammalian normal and cancer cells treated with various compounds to examine whether these substances are capable of protecting the cells against stress caused by rapidly varying temperature, pressure, and radiation, especially UV. Owing to oxidative stress caused by irradiation and temperature shock, we used natural compounds which display antioxidant properties, namely, catechin isolated from green tea, honokiol derived from magnolia, curcumin from turmeric, and cinnamon extract. “After-flight” laboratory tests have shown the most active antioxidants as potential agents which can minimize harmful impact of extreme conditions on human cells.

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“…There is growing evidence that the increase in ROS content is the most common event for various types of cells after cryopreservation (Savitskaya and Onishchenko, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that cryopreservation procedures can induce both apoptosis and necrosis (Savitskaya and Onishchenko, 2016). It was previously shown that during thawing in mesenchymal stem cells, apoptosis was induced by the activation of apoptosis-related proteins including Ca 2+ -dependent protease calpain, caspase-8, -9, and -3 (Bissoyi and Pramanik, 2014).…”

Section: Discussionmentioning

confidence: 99%

Effects of MRI on stemness properties of Wharton’s jelly-derived mesenchymal stem cells

et al. 2022

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BackgroundMesenchymal stem cells (MSCs), derived from various tissues, have served as a promising source of cells in clinic and regenerative medicine. Umbilical cord-Wharton's jelly (WJ-MSCs)-derived MSCs exhibit advantages over those from adult tissues, such as no ethical concerns, shorter population doubling time, broad differentiation potential, readily available non-invasive source, prolonged maintenance of stemness properties. Material and methodsThe aim of this study was to evaluate the effect of MRI (1.5 T, 10 min) on stemness gene expression patterns (OCT-4, SOX-2, NANOG) of WJ-MSCs. In addition, we assessed cell viability, growth kinetics and apoptosis of WJ-MSCs after MRI treatment. ResultsThe quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) data showed that transcript levels of SOX-2, NANOG in MRI-treated WJ-MSCs were increased 32-and 213-fold, respectively.MTT assay was performed at 24, 48, and 72 hours post-treatment and the viability was not signi cantly difference between two groups. The doubling time of MRI group was markedly higher than control group.In addition, the colony formation ability of WJ-MSCs after MRI treatment signi cantly increased. Furthermore, no change in apoptosis was seen before or after MRI treatment. ConclusionsOur results suggest the use of MRI can improve quality of MSCs and may enhance the e cacy of mesenchymal stem cell-based therapies.

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Apoptosis in cryopreserved eukaryotic cells

Cited by 13 publications

References 54 publications

The cytoprotective role of antioxidants in mammalian cells under rapidly varying temperature, pressure and UV conditions during stratospheric balloon campaign

The cytoprotective role of antioxidants in mammalian cells under rapidly varying temperature, pressure and UV conditions during stratospheric balloon campaign

The Cytoprotective Role of Antioxidants in Mammalian Cells Under Rapidly Varying UV Conditions During Stratospheric Balloon Campaign

Effects of MRI on stemness properties of Wharton’s jelly-derived mesenchymal stem cells

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