Apoptosis and proliferation in the inferior colliculus during postnatal development and epileptogenesis in audiogenic Krushinsky–Molodkina rats

Chrysin is the major bioactive compound of blue passionflower, an important medicinal plant used in traditional herbal formulations since ancient times. In the present study, we report that chrysin nanoparticles (chrysin NPs) protect Wistar rats against kindling‐induced epilepsy. Nanoparticles of sizes less than 150 nm with a spherical shape were prepared using poly(d,l‐lactic‐co‐glycolic acid) and polyvinyl alcohol, respectively, as polymer and stabilizer. Rats were injected with subconvulsive doses of pentylenetetrazole (PTZ) (35 mg/kg, intraperitoneal) every second day, with 22 injections in total, and on the same days, they received protective doses of the chrysin NPs (5 and 10 µg/mL, PO), respectively, 45 min before each PTZ injection. After the last PTZ injection, an average of thirteen seizure scores was recorded. Animals were killed by decapitation 24 h after a seizure. The cortex and hippocampus were removed and stored in liquid nitrogen for determining oxidative stress terminal deoxynucleotidyl transferase dUTP nick‐end labeling assay, histopathology, and reverse transcription‐polymerase chain reaction for messenger RNA expression. The result showed chrysin NPs treatment has counteracted oxidative stress, reduced neuronal apoptosis, and upregulated nuclear factor erythroid 2‐related factor 2 (Nrf2), heme oxygenase‐1 (HO‐1), and NAD(P)H quinone oxidoreductase 1. In conclusion, our findings demonstrate that the neuroprotective effect of chrysin NPs against kindling‐induced epilepsy might be escorted by the alleviation of oxidative stress through the Nrf2/antioxidant response element/HO‐1 pathway signal pathway.

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“…The cortex and hippocampus are highly susceptible to seizures and are involved in epileptiform discharges. [ 24‐26 ]…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective role of chrysin‐loaded poly(lactic‐co‐glycolic acid) nanoparticle against kindling‐induced epilepsy through Nrf2/ARE/HO‐1 pathway

Zhang¹,

Zhao

Afzal

et al. 2020

J Biochem & Molecular Tox

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“…In our study, KM rat mitochondrial disorders also induced an increase in the expression of a number of genes that positively regulate apoptosis (Figure 4). An increased apoptosis rate in the inferior colliculi in KM rats compared to W was previously described (Chernigovskaya et al, 2018). This increase in apoptosis rate may be associated with abnormal brain development in KM rats, which in turn may be the cause of epileptogenesis, this effect was experimentally demonstrated in inferior colliculi and hippocampus of KM rats (Chernigovskaya et al, 2018;Kulikov et al, 2020).…”

Section: Discussionmentioning

confidence: 68%

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“…Notably, the increase in the phosphorylation level of ERK1/2 in the KM rats (in comparison with the W and "0" rats) was directly shown in the present work. Previously, elevated levels of phospho-ERK1/2 were demonstrated in the inferior colliculi, hippocampus and striatum of KM rats (Dorofeeva et al, 2017;Chernigovskaya et al, 2018Chernigovskaya et al, , 2019. We assume that activation of ERK1/2 may be a key mechanism for the development of AE.…”

Section: Discussionmentioning

confidence: 76%

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Apoptosis and proliferation in the inferior colliculus during postnatal development and epileptogenesis in audiogenic Krushinsky–Molodkina rats

Cited by 13 publications

References 31 publications

Neuroprotective role of chrysin‐loaded poly(lactic‐co‐glycolic acid) nanoparticle against kindling‐induced epilepsy through Nrf2/ARE/HO‐1 pathway

Neuroprotective role of chrysin‐loaded poly(lactic‐co‐glycolic acid) nanoparticle against kindling‐induced epilepsy through Nrf2/ARE/HO‐1 pathway

Table_1.docx

Image_1.TIF

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