Apoptogenic effects of Tricholoma giganteum on Ehrlich’s ascites carcinoma cell

Abstract

This study explored the efficacy of Fa fraction of Tricholoma giganteum against Ehrlich's ascites carcinoma (EAC). Mechanisms of apoptogenic effect of the fraction were delineated. The flow cytometric analysis of EAC cells, showed an increase in number of cells in sub-G(0)/G(1) population and reduction in the G(2)/M phase due to the treatment thus suggesting apoptosis. The induction of apoptosis has also been confirmed by nuclear staining that demonstrated distinctive morphological features of apoptosis. Our data also revealed an increase in the expression of pro-apoptotic protein p53 in EAC and induced factors contributing to apoptosis. Pro-apoptotic gene Bax was up-regulated during p53-mediated apoptosis. No significant change in the expression of anti-apoptotic protein Bcl-2 was observed ensuing in decrease of the Bcl-2/Bax ratio. p53-mediated growth arrest involves p21 as a major effecter, which interestingly showed moderate elevation. All these observations indicate that Fa fraction of T. giganteum induces apoptogenic signal in EAC.

Regulation of apoptosis through bcl-2/bax proteins expression and DNA damage byZanthoxylum alatum

“…The up-regulation of Bax might restrict the entry of cells into S-phase and can promote nuclear DNA fragmentation (Widlak, 2000). The result shows that treatment with MEZA decreased Bcl-2 and simultaneously increased Bax levels, resulting in the decrease of Bcl-2/Bax ratio (Chatterjee et al, 2013). The balance between the pro-and anti-apoptotic proteins thus shifted towards apoptosis.…”

“…MEZA (2 g/kg) was administered orally to male Swiss albino mice to evaluate the acute toxicity as per the reported method (Chatterjee et al, 2013).…”

Evaluation of Anti-proliferative Potential and Antioxidant Activity of a Wild Edible Mushroom Macrocybe crassa (Sacc.) Pegler and Lodge

“…Mushrooms can grow very easily during the growing season and it is cultivated also in mass amount nowadays, so they are easily available. Those novel bioactive compounds from mushrooms have shown several pharmacological activities like anti-oxidant, 5 antimicrobial, 6 anticancer, 7 immuno-modulatory, 8 anti-diabetic, 9 anti-ulcer, 10 cardio-protective 11 and hepato-protective 12 etc. Free radicals are extremely reactive and highly unstable molecules with an unpaired electron.…”

In Vitro Studies on Antioxidant and Antidiabetic Activity of Pleurotus Eous Mushroom in Methanol and Aqueous Extract

“…It is well known that free radicals are the major cause of various chronic and degenerative diseases, such as coronary heart disease, inflammation, stroke, diabetes mellitus, and cancer [4]. Studies have shown that mushrooms have hepatoprotective [5][6][7], anticancer [8][9][10], antimicrobial [11,12], and antidiabetic [13,14] activities. A study of ancient literature indicates that diabetes (Madhumeha/Prameha) was fairly well-known and well-conceived as an entity in India.…”