Apolipoprotein B messenger RNA editing in rat liver: developmental and hormonal modulation is divergent from apolipoprotein A-IV gene expression despite increased hepatic lipogenesis.

Studies employing human fetal intestine have yielded much interesting information on the role of polarized enterocytes in fat absorption and transport. Using the organ culture model, we examined the influence of hydrocortisone on the synthesis and secretion of lipids and lipoproteins. Human jejunal explants were cultured for 5 days at 37 degrees C in serum-free medium containing either [14C]-oleic acid or [14C]-acetate, alone or supplemented with hydrocortisone (25 or 50 ng/ml). The uptake of [14C]-oleic acid was associated with the production of triglycerides, phospholipids, and cholesteryl esters, which were all affected by hydrocortisone. This hormonal agent (50 micrograms) led to the marked reduction of secreted triglycerides (43%, P < 0.01), phospholipids (39%, P < 0.01), and cholesteryl esters (36%, P < 0.05) without altering the characteristic distribution of tissue and medium lipid classes. Similarly, hydrocortisone significantly (P < 0.01) decreased (approximately 60%) the incorporation of [14C]-acetate into secreted free and esterified cholesterol in the medium. With [14C]-oleic acid as a precursor, hydrocortisone significantly diminished the delivery of chylomicrons and very low density lipoproteins to the medium while consistently enhancing the secretion of high density lipoproteins. In parallel, [35S]-methionine pulse-labeling of jejunal explants revealed the concomitant inhibitory effect of hydrocortisone on apo B-100 synthesis and hydrocortisone's stimulatory effect on apo B-48 and apo A-1. These studies suggest that glucocorticoids play a critical role in lipoprotein processing during intestinal development.

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Selective effects of hydrocortisone on intestinal lipoprotein and apolipoprotein synthesis in the human fetus

Loirdighi

Ménard

Delvin

et al. 1997

J. Cell. Biochem.

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Regulation of apo B mRNA expression in liver and intestine during liver regeneration induced by CCl4

Maldonado¹,

Chan

Melendez³

et al. 1994

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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Hepatic apolipoprotein B synthesis in copper‐deficient rats

Nassir¹,

Mazur²,

Sérougne

et al. 1993

FEBS Letters

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The present study was designed to examine if induction of apolipoprotein B synthesis is associated with hypercholesterolemia in copper-deficient rats. This hypercholesterolemia mainly resides in an increase in the HDL-1 and LDL and is associated with a significant increase in plasma apoB concentration. Liver apoB mRNA levels were not significantly modified in deficient animals as compared to control rats. Studies on liver apolipoprotein synthesis indicated that apoBlO0 synthesis was increased in deficient animals whereas apoB48 synthesis was unchanged. Thus, it appears that the increase in apoB synthesis in the liver of copper-deficient rats occurs at the posttranscriptional level. The selective increase in apoBlO0 synthesis indicates the possible impact of this deficiency on the editing of apoB. An increase in apoBlO0 synthesis by the liver in copper-deficient rats may significantly contribute to the increase in plasma concentration of LDL.

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Apolipoprotein B messenger RNA editing in rat liver: developmental and hormonal modulation is divergent from apolipoprotein A-IV gene expression despite increased hepatic lipogenesis.

Cited by 27 publications

References 37 publications

Selective effects of hydrocortisone on intestinal lipoprotein and apolipoprotein synthesis in the human fetus

Selective effects of hydrocortisone on intestinal lipoprotein and apolipoprotein synthesis in the human fetus

Regulation of apo B mRNA expression in liver and intestine during liver regeneration induced by CCl4

Hepatic apolipoprotein B synthesis in copper‐deficient rats

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