Bacterial infection is considered to be one of the most critical health issues of the world. It is essential to overcome this problem by the development of new drugs. Marine organisms as corals, sponges, seaweeds, and other are an incredible source of novel pharmacologically active compounds. Herein, the antimicrobial activity (extract/fractions) of the invasive stony coral Tubastraea coccinea was evaluated by the disk diffusion method against 21 microbial strains (ATCC and clinical strains). Micro broth dilution was used to determinate the MIC of the fractions that showed antimicrobial activity by the disk diffusion method. Bioautography assay was also performed. Our results showed that the n-butanol (BF) and aqueous fractions (AF) showed activity against ATCC strains Staphylococcus aureus (MIC 31.25 and 250 ÎŒg/mL), Salmonella typhimurium (MIC 125 and 500 ÎŒg/mL), Escherichia coli (MIC 62.5 and 500 ÎŒg/mL) and Pseudomonas aeruginosa (MIC 62.5 * These authors contributed equally to this work. # Corresponding author.
Ă. M. Bianco et al.
335and 500 ÎŒg/mL), respectively. Moreover, BF fraction was also effective against the clinical strains S. aureus (MIC 62.5 ÎŒg/mL), Klebsiella pneumoniae Carbapenemase-KPC (MIC 125 ÎŒg/mL), Methicillin-Resistant Staphylococcus aureus-MRSA (MIC 125 ÎŒg/mL) and Vancomycin-Resistant Enterococcus faecalis-VRE (MIC 62.5 ÎŒg/mL). The ratio MBC/MIC reinforces the bactericidal profile of BF fraction. The bioautography assay of BF fraction showed the presence of antimicrobial components at Rf 0.55.