Aplicações da cultura de tecidos em plantas medicinais

Morais, Tâmara Prado de; Luz, José Magno Queiroz; Silva, S.M.; Resende, Renata F. de; Silva, A.S

doi:10.1590/s1516-05722012000100016

Cited by 35 publications

(19 citation statements)

References 68 publications

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“…Micropropagation protocols are already defined for a great diversity of plant species, from fruit species (RODRIGUES et al, 2013) to medicinal plants (MORAIS et al, 2012), which ensures the sustainable exploitation of species and allows increasing the supply of compounds biologically active, decreasing the price of phytochemical active ingredients derived from medicinal plants (PLETSCH, 1998). Through this technique it is also possible to produce secondary metabolites in vitro, thus ensuring alternative forms of exploitation, especially in endangered ecosystems.…”

Section: Introductionmentioning

confidence: 99%

Antioxidants in the control of microorganism contamination and phenol oxidation in Eugenia pyriformis

Assis¹,

Rodrigues²,

Pasqual³

et al. 2018

Biosci. J.

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Section: Introductionmentioning

confidence: 99%

Antioxidants in the control of microorganism contamination and phenol oxidation in Eugenia pyriformis

Assis¹,

Rodrigues²,

Pasqual³

et al. 2018

Biosci. J.

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“…As such, when well balanced, these compounds can contribute to better plant development and reduced production costs, as well as improve raw material quality (Morais et al, 2012). Based on this information, we sought to assess the effects of different concentrations of mineral salts, charcoal, sucrose, and potassium on the in vitro development of O. basilicum red rubin.…”

Section: Introductionmentioning

confidence: 99%

In vitro cultivation of purple basil Ocimum basilicum L. ‘red rubin’ at different levels of salts, charcoal, sucrose and potassium iodine

Silva¹,

Nascimento²,

Barbosa³

et al. 2017

Aust J Crop Sci

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Ocimum basilicum'red rubin' is highly valued for its essential oil, and is widely used in folk medicine, but few studies examining in vitro propagation techniques for this basil variety are available. We examined the development of red rubin basil seedlings grown in vitro in different concentrations of mineral salts, charcoal combined with sucrose, and potassium iodine in three series of tests. In the first series, five concentrations (0, 25, 50, 75 and 100%) of Murashige and Skoog salts (MS culture medium) were used to germinate sterilized seeds of O. basilicum. In the second test, to evaluate the combined effects of charcoal and sucrose on basil growth, three concentrations of charcoal (0, 3.0, and 4.5 g/L) and two of sucrose (30 and 60 g/L) were used. In the third test, the seeds were sown onto culture medium enriched with five different concentrations of potassium iodine (0, 25, 50, 75 and 100µM); 0.5 μM benzyl aminopurine (BAP) and 1.0 μM naphthalene acetic acid (NAA) were added to all germination media. A completely randomized experimental design was used in all tests, with five replicates, four vials per test, and 4 seeds per vial. In Test 2, seedling oxidation (%) and the numbers of leaves and shoots were submitted to non-parametric analysis using the Kruskal Wallis test (p ≤0.05). The other data were submitted to analysis of variance (p≤0.05). The qualitative means were evaluated by the Tukey test (p≤0.05), and the quantitative means by polynomial regression (p≤0.05). The results showed that 100% MS culture medium was most indicated for 'red rubin' seed germination and aerial portion production, while 70-80% MS was indicated for root production. A sucrose level of 30 g/L (standard) combined with 4.5 g/L of activated charcoal allowed the best seedlings development and inhibited callus formation. Activated charcoal limited copper and zinc uptake by O. basilicum seedlings, but not enough to hamper their development. Increasing concentrations of potassium iodine reduced all of the growth variables analyzed in this basil variety.Keywords: Ocimum basilicum L., micropropagation, medicinal plant, culture medium. Abbreviation: AC_ activated charcoal, APL_ aerial portion length, BAP_ benzylaminopurine, Cu_ Copper; CSD1 and CSD2_ gene families in Arabidopsis, DMAP_ dry mass of the aerial portion, DMRC_ dry mass of the root or of the root + callus portion, FMAP_ fresh masses of the aerial portions, FMRC_ fresh masses of the root, KI_ potassium iodide, miRNAs_ micro RNAs, MS_ Murashige and Skoog, NAA_ naphthalene acetic acid, NC-presence of callus, NL_ number of leaves, NS_ numbers of shoots, OX_ oxidized plants, PVP_polyvinyl pyrrolidone, RL_ root, SISVAR_ statistical analysis system, SOD_ superoxide dismutase, Zn_ Zinc.

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“…Apesar dos grandes avanços observados na medicina moderna, as plantas medicinais ainda desempenham importante papel na saúde mundial (18). Existem obstá-culos na utilização de medicamentos pela população de diferentes países em desenvolvimento, que vão desde o acesso aos hospitais até o difícil acesso ao tratamento.…”

Section: Introductionunclassified

PERFIL FITOQUÍMICO, SUSCEPTIBILIDADE ANTIBACTERIANA E CAPACIDADE ANTIOXIDANTE DAS FOLHAS DE Croton urucurana Baillon (Euphorbiaceae)

Silva

Alves²,

Sousa³

et al. 2017

Infarma

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Croton urucurana Baillon, popularly known as "sangria d'água", belongs to the Euphorbiaceae family. The species has a great importance in folk medicine as antibacterial, anti-inflammatory, antiseptic, antiviral, antidiarrheal, intestinal antisecretory and cicatrizant remedies. The objective of this study was to verify the presence, in the aqueous, ethanolic and hydroalcoholic extracts, of secondary metabolites (alkaloids, anthraquinones, steroids/triterpenoids, flavonoids, glycosides, saponins and tannins), analyze the inhibition of bacteria Staphylococcus aureus ATCC 25923 and Salmonella thyphimurium ATCC 14028 growth, and evaluate the antioxidant activity by the DPPH method. Considering the phytochemical profile, the best extract was the aqueous; the bacteria susceptibility was inhibited with the ethanolic extract; and the extract with the highest antioxidant activity was the hydroalcoholic, presenting IC 50 1.3 μg/ mL. It was concluded that the extracts of Croton urucurana Baillon show interesting results and that further investigations must be carried out for the better elucidation of its benefits. Keywords:Croton urucurana Baillon; phytochemical; antioxidant; antibacterial; extract. RESUMOCroton urucurana Baillon, espécie conhecida popularmente como "sangria d'água", pertence à família Euphorbiaceae, possui grande importância na medicina popular, a qual lhe atribui propriedades antibacterianas, anti-inflamató-rias, antissépticas, antivirais, antidiarreicas, antissecretória intestinal e cicatrizante. Os objetivos deste estudo foram verificar a presença, nos extratos aquoso, etanólico e hidroalcoólico, de metabolitos secundários (alcaloides, antraquinonas, esteroides/triterpenoides, flavonoides, glicosídeos, saponinas e taninos), analisar a inibição do crescimento das bactérias Staphylococcus aureus ATCC 25923 e Salmonella thyphimurium ATCC 14028, e verificar a atividade antioxidante através do método DPPH. Para o perfil fitoquímico o melhor extrato foi o aquoso, quanto à susceptibilidade bacteriana, houve inibição com o extrato etanólico e o extrato com a maior atividade antioxidante foi o hidroalcóolico, apresentando IC 50 1,3 μg/mL. Considerando os resultados obtidos no estudo, foi possível concluir que os extratos da Croton urucurana Baillon apresentam resultados interessantes e que mais investigações devem ser realizadas para a elucidação aprimorada dos seus benefícios.

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Aplicações da cultura de tecidos em plantas medicinais

Cited by 35 publications

References 68 publications

Antioxidants in the control of microorganism contamination and phenol oxidation in Eugenia pyriformis

Antioxidants in the control of microorganism contamination and phenol oxidation in Eugenia pyriformis

In vitro cultivation of purple basil Ocimum basilicum L. ‘red rubin’ at different levels of salts, charcoal, sucrose and potassium iodine

PERFIL FITOQUÍMICO, SUSCEPTIBILIDADE ANTIBACTERIANA E CAPACIDADE ANTIOXIDANTE DAS FOLHAS DE Croton urucurana Baillon (Euphorbiaceae)

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