Aphid transmission of cauliflower mosaic virus requires the viral PIII protein

Leh, Véronique; Jacquot, Emmanuel; Geldreich, Angèle; Hermann, Thomas; Leclerc, Denis; Cérutti, Martine; Yot, P.; Keller, Mario; Blanc, Stéphane

doi:10.1093/emboj/18.24.7077

Cited by 84 publications

(72 citation statements)

References 28 publications

(43 reference statements)

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“…Previous results (10,14) clearly indicated that P2 bound via P3 to virions constitutes the transmissible complex. We believe that the data presented here enlarge the so far static picture of this complex by giving important insights into dynamics and regulation of its formation with far-reaching consequences for the mode of CaMV transmission by aphids.…”

Section: Discussionmentioning

confidence: 90%

“…2 C and D). When the P2 157m mutant (24), which does not bind P3 in vitro (10), was coexpressed with P3 in Sf9 cells, no such inclusion bodies were observed and P3 remained evenly dispersed throughout the cell (not shown). Thus, our results indicate that a P2:P3 complex exists in Sf9 cells.…”

Section: Resultsmentioning

confidence: 99%

“…Because a possible P2:P3 complex has not been reported in plant cells before, although in vitro data (10) indicate that it might exist, we sought to confirm its existence in vivo. With this aim, we coinfected Sf9 cells with P2-and P3-encoding baculoviruses.…”

Section: Resultsmentioning

confidence: 99%

“…It was recently shown, however, that P2 alone cannot mediate aphid transmission of CaMV. In addition, the action of the viral gene III product P3 is needed (10). This 15-kDa protein had earlier been reported to copurify with virus particles and be essential for virus infectivity, although no particular function was attributed to it (11)(12)(13).…”

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confidence: 99%

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Intracellular distribution of viral gene products regulates a complex mechanism of cauliflower mosaic virus acquisition by its aphid vector

Drucker

Froissart

Hébrard

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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Interactions between Cauliflower mosaic virus (CaMV) and its aphid vector are regulated by the viral protein P2, which binds to the aphid stylets, and protein P3, which bridges P2 and virions. By using baculovirus expression of P2 and P3, electron microscopy, surface plasmon resonance, affinity chromatography, and transmission assays, we demonstrate that P3 must be previously bound to virions in order that attachment to P2 will allow aphid transmission of CaMV. We also show that a P2:P3 complex exists in the absence of virions but is nonfunctional in transmission. Hence, unlike P2, P3 and virions cannot be sequentially acquired by the vector. Immunogold labeling revealed the predominance of spatially separated P2:P3 and P3:virion complexes in infected plant cells. This specific distribution indicates that the transmissible complex, P2:P3:virion, does not form primarily in infected plants but in aphids. A model, describing the regulating role of P3 in the formation of the transmissible CaMV complex in planta and during acquisition by aphids, is presented, and its consequences are discussed.

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Section: Discussionmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Intracellular distribution of viral gene products regulates a complex mechanism of cauliflower mosaic virus acquisition by its aphid vector

Drucker

Froissart

Hébrard

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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“…Interestingly, a coiled-coil domain promotes the interaction between the PIII and PII proteins of Cauliflower mosaic virus during transmission by vector aphids (Leclerc et al, 1998 ;Leh et al, 1999). Two mutations of the PEBV TpA56 2b protein are known to severely affect nematode transmission of the virus.…”

Section: Tobraviruses Which Include Pea Early-browning Virus (Pebv)mentioning

confidence: 99%

Substitution of a single amino acid in the 2b protein of Pea early-browning virus affects nematode transmission

Vellios

Brown

MacFarlane

2002

Journal of General Virology

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The 2b protein of Pea early-browning virus (PEBV) is required for transmission of the virus by nematodes. Comparison of the 2b proteins of highly transmissible (TpA56) and poorly transmissible (SP5) isolates of PEBV identified two amino acid substitutions (G90S and G177R) that might be responsible for the poor transmission of isolate SP5. Hybrid viruses were created in which the TpA56 2b protein carried SP5-specific substitutions at residue 90 or 177, and in which the SP5 2b protein carried TpA56-specific substitutions at these positions. Transmission tests showed that the G177R substitution is sufficient to prevent nematode transmission of the virus. Examination of the 2b proteins from PEBV and other tobraviruses predicted the presence of a coiled-coil domain in the central region of the protein. This structural element is important for the association of interacting proteins and, thus, might mediate interaction of the 2b protein with the virus coat protein or with the vector nematode.Tobraviruses, which include Pea early-browning virus (PEBV), Tobacco rattle virus (TRV) and Pepper ringspot virus (PepRSV) are transmitted between plants by soil-inhabiting nematodes (Taylor & Brown, 1997). Glasshouse tests have demonstrated that particular tobravirus isolates can be transmitted only by certain nematode species. For example, TRV isolate PpK20 can be transmitted by Paratrichodorus pachydermus but not by P. anemones or Trichodorus primitivus (Herna! ndez et al., 1997). In contrast, TRV isolate PaY4 can be transmitted by both P. pachydermus and P. anemones (Vassilakos, 2000 ; Vassilakos et al., 2001), whereas PEBV isolate TpA56 is transmitted by T. primitivus but not by P. pachydermus (MacFarlane & Brown, 1995).Tobraviruses have two positive-stranded genomic RNAs, each of which is encapsidated separately in a rod-shaped particle. The larger RNA (RNA1) encodes proteins involved in Author for correspondence : Stuart MacFarlane.Fax j44 1382 562426. e-mail s.macfarlane!scri.sari.ac.uk the replication and spread of the virus in plants, and can infect plants systemically in the complete absence of the second, smaller genomic RNA (RNA2) (Harrison & Robinson, 1986). RNA2 was shown to carry the determinants for nematode transmission by the analysis of pseudorecombinant isolates of TRV in which the genomic RNAs from the nematodetransmissible PpK20 isolate and the non-transmissible PLB isolate were re-assorted (Ploeg et al., 1993). These experiments showed that inclusion of RNA2 from TRV PpK20 led to the transmission of TRV PLB RNA1, and the replacement of PpK20 RNA2 with PLB RNA2 resulted in failure to transmit PpK20 RNA1. Thus, RNA2 of TRV PpK20 encoded a transmission determinant(s) that was lacking in RNA2 of TRV PLB.RNA2 has been sequenced from many tobravirus isolates, revealing a great variation in the size and gene content (MacFarlane, 1999). All isolates encode a coat protein (CP), although deleted forms of RNA2 lacking the CP gene can be generated in infected plants (Herna! ndez et al., 1996). Many isolates include at ...

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Caulimoviridae (Plant Pararetroviruses)

Hull

2007

Encyclopedia of Life Sciences

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Aphid transmission of cauliflower mosaic virus requires the viral PIII protein

Cited by 84 publications

References 28 publications

Intracellular distribution of viral gene products regulates a complex mechanism of cauliflower mosaic virus acquisition by its aphid vector

Intracellular distribution of viral gene products regulates a complex mechanism of cauliflower mosaic virus acquisition by its aphid vector

Substitution of a single amino acid in the 2b protein of Pea early-browning virus affects nematode transmission

Caulimoviridae (Plant Pararetroviruses)

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