Apelin stimulates proliferation and suppresses apoptosis of mouse osteoblastic cell line MC3T3-E1 via JNK and PI3-K/Akt signaling pathways

Tang, Siyuan; Xie, Hui; Yuan, Ling‐Qing; Luo, Xi; Huang, Jiao; Cui, Rongrong; Zhou, Hong-Hao; Wu, Xianping; Liao, Er-Yuan

doi:10.1016/j.peptides.2006.10.005

Cited by 113 publications

(82 citation statements)

References 58 publications

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“…The anti-apoptotic action of taurine in osteoblasts may contribute to its bone-sparing effect. (32)(33)(34). Moreover, there have been reports showing that taurine was capable of suppressing apoptosis through the mitochondrial pathway in retinal ganglion cells (35), hepatocytes (36), and testicular tissue (37).…”

Section: Discussionmentioning

confidence: 99%

Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway

Zhang¹,

Zhou²,

Chen³

et al. 2011

Braz J Med Biol Res

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Taurine has positive effects on bone metabolism. However, the effects of taurine on osteoblast apoptosis in vitro have not been reported. The aim of this study was to investigate the activity of taurine on apoptosis of mouse osteoblastic MC3T3-E1 cells. The data showed that 1, 5, 10, or 20 mM taurine resulted in 16.7, 34.2, 66.9, or 63.75% reduction of MC3T3-E1 cell apoptosis induced by the serum deprivation (serum-free α-MEM), respectively. Taurine (1, 5, or 10 mM) also reduced cytochrome c release and inhibited activation of caspase-3 and -9, which were measured using fluorogenic substrates for caspase-3/caspase-9, in serum-deprived MC3T3-E1 cells. Furthermore, taurine (10 mM) induced extracellular signal-regulated kinase (ERK) phosphorylation in MC3T3-E1 cells. Knockdown of the taurine transporter (TAUT) or treatment with the ERK-specific inhibitor PD98059 (10 μM) blocked the activation of ERK induced by taurine (10 mM) and abolished the anti-apoptotic effect of taurine (10 mM) in MC3T3-E1 cells. The present results demonstrate for the first time that taurine inhibits serum deprivation-induced osteoblast apoptosis via the TAUT/ERK signaling pathway

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Section: Discussionmentioning

confidence: 99%

Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway

Zhang¹,

Zhou²,

Chen³

et al. 2011

Braz J Med Biol Res

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“…DNA and protein synthesis assay DNA and protein synthesis were assessed using scintillation counting to measure the incorporation of [ 3 H]thymidine (7.4×10 4 Bq/mL) or [ 3 H]leucine into DNA or protein, respectively, as previously described [29][30][31] . Briefly, cells were plated at a density of 2×10 4 /well in 24-well plates, cultured in growth medium and treated with vehicle controls, 5−20 mmol/L TAU, 1−10 mmol/L LC or 20 mmol/L TAU+10 mmol/L LC for 48 h. Then, the plates were washed with PBS, and 10% trichloroacetic acid solution was added to the wells to precipitate the DNA and protein.…”

Section: Methodsmentioning

confidence: 99%

L-carnitine and taurine synergistically inhibit the proliferation and osteoblastic differentiation of vascular smooth muscle cells

et al. 2010

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Aim: To investigate the synergistic action of L-carnitine (LC) and taurine (TAU) on the proliferation and osteoblastic differentiation of vascular smooth muscle cells (VSMCs). Methods: DNA and protein synthesis of VSMCs were assessed using scintillation counting. Alkaline phosphatase (ALP) activity and calcium content were determined to investigate the effects of LC and TAU on the osteoblastic differentiation and mineralization of VSMCs. TAU uptake by VSMCs was assayed. RNA interference was used to down-regulate the expression of the TAU transporter (TAUT) in rat VSMCs. Results: LC and TAU synergistically inhibited the proliferation and β-glycerophosphate (β-GP)-induced osteoblastic differentiation of VSMCs as evidenced by the decreased [ 3 H]thymidine incorporation, ALP activity and calcium deposition. Furthermore, LC stimulated the TAU uptake and TAUT expression in VSMCs. Suppression of TAUT with short hairpin RNA (shRNA) abolished the synergistic action of LC and TAU in VSMCs. Conclusion: The synergistic inhibitory action of LC and TAU on the proliferation and osteoblastic differentiation of VSMCs is attributable to the up-regulation of TAUT expression and TAU uptake by LC.

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“…Other investigations have shown that VISF increases type I collagen production in human osteoblast cultures [40] while APE stimulates proliferation and suppresses apoptosis of mouse osteoblastic cell line MC3T3-E1 [41]. The latter also has a protective effect on cultured rat bone marrow mesenchymal stem cells against apoptosis [42].…”

Section: Introductionmentioning

confidence: 93%

Metabolizm kostny, osteoprotegeryna i ligand receptora aktywatora czynnika jądrowego-kB a wybrane hormony tkanki tłuszczowej u dziewcząt z jadłowstrętem psychicznym

Ostrowska¹,

Ziora²,

Oświęcimska³

et al. 2014

Endokrynologia Polska

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Apelin stimulates proliferation and suppresses apoptosis of mouse osteoblastic cell line MC3T3-E1 via JNK and PI3-K/Akt signaling pathways

Cited by 113 publications

References 58 publications

Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway

Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway

L-carnitine and taurine synergistically inhibit the proliferation and osteoblastic differentiation of vascular smooth muscle cells

Metabolizm kostny, osteoprotegeryna i ligand receptora aktywatora czynnika jądrowego-kB a wybrane hormony tkanki tłuszczowej u dziewcząt z jadłowstrętem psychicznym

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