Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway

Zhang, Leiyi; Zhou, Yanqing; Chen, Fei; Wang, Bing; Li, Jing; Deng, Youwen; Liu, Weidong; Wang, Zhengguang; Li, Yawei; Li, Dong-Zhe; Li, Guohua; Yin, Bin

doi:10.1590/s0100-879x2011000700002

Cited by 6 publications

(4 citation statements)

References 16 publications

(18 reference statements)

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“…Low molecular weight PEGDA microspheres had the lowest cell viability. It is possible that large serum proteins could not pass through the smaller mesh size that comprises low molecular weight PEGDA, resulting in the increased cell death observed (Zhang et al 2011). The various mesh sizes were calculated for these hydrogels and found to be 32.3, 28, 16.2 (Pellegrini et al 2015), and 10.8 nm for 20, 10, 5, and 3.4 kDa molecular weight PEGDA, respectively.…”

Section: Discussionmentioning

confidence: 99%

The effect of polymer molecular weight and cell seeding density on viability of cells entrapped within PEGDA hydrogel microspheres

Perera

Medini

Seethamraju

et al. 2018

Journal of Microencapsulation

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Cell microencapsulation can be used in tissue engineering as a scaffold or physical barrier that provides immunoisolation for donor cells. When used as a barrier, microencapsulation shields donor cells from the host immune system when implanted for cell therapies. Maximizing therapeutic product delivery per volume of microencapsulated cells necessitates first optimising the viability of entrapped cells. Although cell microencapsulation within alginate is well described, best practices for cell microencapsulation within polyethylene glycol is still being elucidated. In this study we microencapsulate mouse preosteoblast cells within polyethylene glycol diacrylate (PEGDA) hydrogel microspheres of varying molecular weight or seeding densities to assess cell viability in relation to cell density and polymer molecular weight. Diffusion studies revealed molecule size permissible by each molecular weight PEGDA towards correlating viability with polymer mesh size. Results demonstrated higher cell viability in higher molecular weight PEGDA microspheres and when cells were seeded at higher cell densities.

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Section: Discussionmentioning

confidence: 99%

The effect of polymer molecular weight and cell seeding density on viability of cells entrapped within PEGDA hydrogel microspheres

Perera

Medini

Seethamraju

et al. 2018

Journal of Microencapsulation

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“…We have previously demonstrated that claudin-1 mediates its pro-carcinogenic and metastatic effects through the activation of Src and Akt signaling, and claudin-7 regulates ERK signaling to modulate EMT in colon cancer [ 17 , 52 , 53 ]. These pathways are known to regulate PIK3C, SLC6A6, and ASAP-1, while less is known about the regulation of TMEM-43 [ 53 , 54 , 55 ]. Future studies are needed to investigate the role of these signaling pathways in claudin-1 and 7’s regulation of PIK3CA, SLC6A6, ASAP-1, and TMEM-43.…”

Section: Discussionmentioning

confidence: 99%

A Claudin-Based Molecular Signature Identifies High-Risk, Chemoresistant Colorectal Cancer Patients

Gowrikumar

Primeaux

Pravoverov

et al. 2021

Cells

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Identifying molecular characteristics that are associated with aggressive cancer phenotypes through gene expression profiling can help predict treatment responses and clinical outcomes. Claudins are deregulated in colorectal cancer (CRC). In CRC, increased claudin-1 expression results in epithelial-to-mesenchymal transition and metastasis, while claudin-7 functions as a tumor suppressor. In this study, we have developed a molecular signature based on claudin-1 and claudin-7 associated with poor patient survival and chemoresistance. This signature was validated using an integrated approach including publicly available datasets and CRC samples from patients who either responded or did not respond to standard-of-care treatment, CRC cell lines, and patient-derived rectal and colon tumoroids. Transcriptomic analysis from a patient dataset initially yielded 23 genes that were differentially expressed along with higher claudin-1 and decreased claudin-7. From this analysis, we selected a claudins-associated molecular signature including PIK3CA, SLC6A6, TMEM43, and ASAP-1 based on their importance in CRC. The upregulation of these genes and their protein products was validated using multiple CRC patient datasets, in vitro chemoresistant cell lines, and patient-derived tumoroid models. Additionally, blocking these genes improved 5-FU sensitivity in chemoresistant CRC cells. Our findings propose a new claudin-based molecular signature that associates with poor prognosis as well as characteristics of treatment-resistant CRC including chemoresistance, metastasis, and relapse.

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“…Previous studies revealed that Nrf2 was able to regulate cytoprotective genes and cellular antioxidant proteins, as well as allow cells to adapt to stress induced by electrophiles and oxidants (42)(43)(44). To analyze the mechanism of action of curcumin on HG-induced EMT in the NRK-52E cells in the present study, the nuclear accumulation of Nrf2 protein in the curcumin-treated NRK-52E cells was examined.…”

Section: Curcumin Increases Nrf2 Expression In Nrk-52e Cellsmentioning

confidence: 96%

Curcumin protects renal tubular epithelial cells from high glucose-induced epithelial-to-mesenchymal transition through Nrf2-mediated upregulation of heme oxygenase-1

Zhang

Liang

Guo

et al. 2012

Molecular Medicine Reports

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Curcumin has been observed to exhibit an anti-fibrotic effect in the liver, lung and gallbladder. However, the mechanisms underlying the cytoprotective effects of curcumin remain to be elucidated. The epithelial-to-mesenchymal transition (EMT) of mature tubular epithelial cells in the kidney is considered to contribute to the renal accumulation of matrix proteins associated with diabetic nephropathy. The EMT is also closely associated with the progression of renal interstitial fibrosis and oxidative stress. This process may occur through abrogation of high glucose (HG)-induced oxidative stress via activation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO-1) in kidney tubular epithelial cells. In the present study, the effect of curcumin on HG-induced EMT in the NRK-52E normal rat kidney tubular epithelial cell line was investigated, and whether the effect of curcumin was mediated by the induction of Nrf2 and HO-1 expression was examined. The present study revealed that curcumin was able to prevent events associated with EMT, including the downregulation of E-cadherin and the increased expression of α-smooth muscle actin. Further analysis revealed that the expression levels of Nrf2 and HO-1 protein were elevated to a greater extent in the curcumin pretreated NRK-52E cells compared with those of the control. Notably, knockdown of Nrf2 with small interfering RNA prevented the curcumin-induced elevation in expression of HO-1 and the associated anti-fibrotic effects. In conclusion, the present findings suggested that curcumin may be significant in cellular antioxidant defense, through the activation of Nrf2 and HO-1, thereby protecting the NRK-52E cells from HG-induced EMT.

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Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway

Cited by 6 publications

References 16 publications

The effect of polymer molecular weight and cell seeding density on viability of cells entrapped within PEGDA hydrogel microspheres

The effect of polymer molecular weight and cell seeding density on viability of cells entrapped within PEGDA hydrogel microspheres

A Claudin-Based Molecular Signature Identifies High-Risk, Chemoresistant Colorectal Cancer Patients

Curcumin protects renal tubular epithelial cells from high glucose-induced epithelial-to-mesenchymal transition through Nrf2-mediated upregulation of heme oxygenase-1

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