Apatite formation on bioactive calcium-silicate cements for dentistry affects surface topography and human marrow stromal cells proliferation

Gandolfi, Maria Giovanna; Ciapetti, G.; Taddei, Paola; Perut, Francesca; Tinti, Anna; Cardoso, Marcio Vivan; Meerbeek, Bart Van; Prati, Carlo

doi:10.1016/j.dental.2010.06.002

Cited by 174 publications

(192 citation statements)

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“…It has been reported that MTA consists mainly of various calcium compounds such as calcium silicates (Ca 3 SiO 5 , Ca 2 SiO 4 , CaSiO 3 ), calcium aluminate (CaAl 2 O 4 ), and calcium sulfate (CaSO 4 •2H 2 O) [9] [11]. Calcium silicates in particular have been reported to have excellent antibacterial, hard tissue regeneration, and bone conductivity properties [12] [13]. Specifically, dicalcium silicate [11] or tricalcium silicate [13]- [15] produces calcium hydroxide in chemical reactions with water, and hardens MTA [9].…”

Section: Introductionmentioning

confidence: 99%

“…Calcium silicates in particular have been reported to have excellent antibacterial, hard tissue regeneration, and bone conductivity properties [12] [13]. Specifically, dicalcium silicate [11] or tricalcium silicate [13]- [15] produces calcium hydroxide in chemical reactions with water, and hardens MTA [9]. Excellent properties are obtained as a result of the strong alkalinity of calcium hydroxide [11] [16] [17].…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of CaO-SiO<sub>2</sub> Compounds Using Materials Extracted from Industrial Wastes

Yamaguchi¹,

Masuda²,

Yamada³

et al. 2015

OJINM

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Mineral trioxide aggregate (MTA) cement is an attractive material in endodontic dentistry. The purpose of this study was to produce calcium silicate, which is a major component of MTA, from waste materials. A dental alginate impression gel and used chalks were selected and mixed in a suitable ratio (Code: EXP). As a control, CaCO3 and a commercial diatomite were used (Code: CON). Each powder was heated to 850˚C and 1000˚C, and then kneaded with water. TG-DTA, compressive tests, SEM observations, elemental mapping analyses, and XRD analyses were performed. TG-DTA indicated that weight reduction of CaCO3 started at 600˚C, and it completely decomposed on heating at 850˚C. The strength was affected by the temperature. After heating, CaCO3 was transformed into CaO and/or Ca2SiO4, and Ca(OH)2 was formed by mixing with water. There were no differences between EXP and CON. These data suggested that recycled wastes might be promising MTA sources.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis of CaO-SiO<sub>2</sub> Compounds Using Materials Extracted from Industrial Wastes

Yamaguchi¹,

Masuda²,

Yamada³

et al. 2015

OJINM

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“…With this approach, TCP based materials can either decrease 21) or increase cellular parameters in vitro 22,23) . TCP extracts caused moderately stimulated cell viability, proliferation and osteogenic differentiation 24,25) .…”

Section: Discussionmentioning

confidence: 99%

In Vitro Cell Viability Tests on a Composite Graft Containing Alpha Tricalcium Phosphate, Chondroitin Sulfate and Disodium Succinate

Brolese

Buser

Benoit

et al. 2014

J. Hard Tissue Biology.

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A granular bone substitute composed of alpha tricalcium phosphate, chondroitin sulfate and disodium succinate is biocompatible in vivo. The aim of this in vitro study was to analyze cellular aspects related to bone regeneration. The response of MG63 and L929 cells to liquid extracts of the respective material was reported. Viability and integrity of the cell membrane were determined by MTT and trypan blue exclusion assay. Cytoskeletal changes were followed by phalloidin staining. Expression of apoptotic, mitogenic and osteogenic differentiation genes was detected by RT-PCR. Supernatants obtained at 24 hours of extraction, but not later, reduced formazan formation in L929 and MG63 cells (p<0.05). Also sodium chondroitin sulfate at 10 % and disodium succinate at 2.5% significantly decreased cell viability (p<0.05). Moreover, we observed impaired integrity of the cell membrane and cytoskeletal changes. Expression of mitogenic genes was partially reduced while apoptotic and differentiation genes were unchanged. Liquid extracts of TCP and TCP failed to cause any substantial effects. Thus, the composite bone substitute can cause an early, but temporary cell necrosis. Since bone regeneration is initiated by a transient catabolic phase, the observed effects are in line with this biological principle.

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“…70 Dulbeco's phosphate buffered saline was used as the mixing liquid. The cement displayed high bioactivity and human marrow stromal cell proliferation.…”

Section: Other Calcium Silicate Materialsmentioning

confidence: 99%

Calcium silicate materials in endodontics

Dutta¹,

Saunders

2014

Dent Update

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Calcium silicate materials have been recently introduced to dentistry and have found wide applications in endodontics because of their favourable biological properties. This review discusses materials that have become available commercially as well as those that are currently experimental. The compositional aspects of Mineral Trioxide Aggregate (MTA) are discussed with modifications and the development of newer materials. Based upon this information, a definition and classification for calcium silicate materials has been proposed. Calcium silicate materials have properties that make them suitable for use in endodontics. Research with hybrid materials may lead to the development of a cement with more desirable characteristics. Clinical Relevance: Dentists should be aware of the chemistry of the calcium silicate group of materials that includes Mineral Trioxide Aggregate and several newer materials.

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Apatite formation on bioactive calcium-silicate cements for dentistry affects surface topography and human marrow stromal cells proliferation

Cited by 174 publications

References 50 publications

Synthesis of CaO-SiO<sub>2</sub> Compounds Using Materials Extracted from Industrial Wastes

Synthesis of CaO-SiO<sub>2</sub> Compounds Using Materials Extracted from Industrial Wastes

In Vitro Cell Viability Tests on a Composite Graft Containing Alpha Tricalcium Phosphate, Chondroitin Sulfate and Disodium Succinate

Calcium silicate materials in endodontics

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Apatite formation on bioactive calcium-silicate cements for dentistry affects surface topography and human marrow stromal cells proliferation

Cited by 174 publications

References 50 publications

Synthesis of CaO-SiO&lt;sub&gt;2&lt;/sub&gt; Compounds Using Materials Extracted from Industrial Wastes

Synthesis of CaO-SiO&lt;sub&gt;2&lt;/sub&gt; Compounds Using Materials Extracted from Industrial Wastes

In Vitro Cell Viability Tests on a Composite Graft Containing Alpha Tricalcium Phosphate, Chondroitin Sulfate and Disodium Succinate

Calcium silicate materials in endodontics

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Product

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Synthesis of CaO-SiO<sub>2</sub> Compounds Using Materials Extracted from Industrial Wastes

Synthesis of CaO-SiO<sub>2</sub> Compounds Using Materials Extracted from Industrial Wastes