AP-1 regulates the expression of IL17-4 and IL17-5 in the pacific oyster Crassostrea gigas

Wang, Liyan; Sun, Jiejie; Wu, Zeyan; Lian, Xingye; Han, Shuo; Huang, Shu; Yang, Chuanyan; Song, Linsheng

doi:10.1016/j.fsi.2019.12.080

Cited by 19 publications

(5 citation statements)

References 59 publications

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“…The AP‐1 protein functions as a determinant in response to oxidative stress and stress tolerance 38 . In the Pacific oyster Crassostrea gigas , the mRNA transcripts of AP‐1 in hemocytes significantly increase after lipopolysaccharides stimulation 39 . Here, we report, for the first time, the induced expression of AP‐1 in insects owing to insecticide exposure.…”

Section: Discussionmentioning

confidence: 76%

Activator protein‐1 mediated CYP6ER1 overexpression in the clothianidin resistance of Nilaparvata lugens (Stål)

Jin

Wang

et al. 2021

Pest Management Science

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BACKGROUND Nilaparvata lugens, a destructive rice pest in Asia, has developed resistance to many insecticides, including the neonicotinoid clothianidin. CYP6ER1 plays an important role in N. lugens resistant to clothianidin, but only limited information on the transcriptional regulation of CYP6ER1 overexpression in clothianidin resistance is available. RESULTS In this study, the transcription factor activator protein 1 (AP‐1) was found to be overexpressed in a clothianidin‐resistant strain of N. lugens and several field resistant populations. RNA interference‐mediated silencing of NlAP‐1 significantly decreased CYP6ER1 expression and increased the susceptibility of N. lugens to clothianidin. Additionally, NlAP‐1 was highly expressed in egg and adult stages, and in midguts, and NlAP‐1 was upregulated and induced to a greater extent in the clothianidin‐resistant strain after exposure to clothianidin. Finally, dual‐luciferase reporter assays confirmed the interaction between NlAP‐1 and the two predicted binding sites in the CYP6ER1 promoter. CONCLUSION NlAP‐1 bound the −1388 to −1208‐bp region of the CYP6ER1 promoter, enhancing its activity and then regulate the expression of CYP6ER1. These findings enhance our knowledge of the transcriptional regulation of the P450 genes that mediate insecticide resistance in insect pests. © 2021 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 76%

Activator protein‐1 mediated CYP6ER1 overexpression in the clothianidin resistance of Nilaparvata lugens (Stål)

Jin

Wang

et al. 2021

Pest Management Science

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“…IL‐17 plays a wide variety of immunoregulatory roles, and IL‐17 deficiency leads to exacerbated diseases during infection 27‐35 . Recent studies have indicated the ubiquity of IL‐17 family members throughout the animal kingdom from mollusk and teleost to mammals, and their potential involvement in the immune response has been preliminarily demonstrated 44,46,47,58‐61 . For example, at least six IL‐17s were identified from Pacific oyster Crassostrea gigas , and it was shown that the AP‐1 or ERK–Rel axis positively regulated, and SOCS6 negatively regulated IL‐17 expression in hemocytes after LPS stimulation 58‐61 .…”

Section: Discussionmentioning

confidence: 99%

“…[27][28][29][30][31][32][33][34][35] Recent studies have indicated the ubiquity of IL-17 family members throughout the animal kingdom from mollusk and teleost to mammals, and their potential involvement in the immune response has been preliminarily demonstrated. 44,46,47,[58][59][60][61] For example, at least six IL-17s were identified from Pacific oyster Crassostrea gigas, and it was shown that the AP-1 or ERK-Rel axis positively regulated, and SOCS6 negatively regulated IL-17 expression in hemocytes after LPS stimulation. [58][59][60][61] Distinct families of IL-17 were also identified from various teleost species, and their mRNA expression was observed to be markedly induced by bacterial or viral infection.…”

Section: Discussionmentioning

confidence: 99%

“…44,46,47,[58][59][60][61] For example, at least six IL-17s were identified from Pacific oyster Crassostrea gigas, and it was shown that the AP-1 or ERK-Rel axis positively regulated, and SOCS6 negatively regulated IL-17 expression in hemocytes after LPS stimulation. [58][59][60][61] Distinct families of IL-17 were also identified from various teleost species, and their mRNA expression was observed to be markedly induced by bacterial or viral infection. 44,46,47 However, few studies have revealed the involvement of IL-17 in adaptive immunity in early vertebrates, especially the T-cell immunity.…”

Section: Discussionmentioning

confidence: 99%

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Fish NF‐κB couples TCR and IL‐17 signals to regulate ancestral T‐cell immune response against bacterial infection

et al. 2021

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As fish constitute the first evolutionary group with primordial T cells, they are of importance for understanding the origin and evolution of adaptive immunity. Yet, the knowledge about how ancestral T cells function remains limited. Therefore, the teleost model Nile tilapia (Oreochromis niloticus) was used in this study to investigate the regulatory mechanisms of T-cell immunity in fish. We identified an evolutionarily conserved canonical NF-κB signaling pathway in Nile tilapia, which participates in primary adaptive immune response during Streptococcus agalactiae infection. Blockade of NF-κB activity severely impairs T-cell activation and expansion, rendering the animals more vulnerable to pathogen attack. Meanwhile, NF-κB signaling is indispensable for fish T cells to produce IL-17A during the antibacterial immune response. Moreover, IL-17A binds its receptor IL-17RA, initiates the ACT1-TRAF6-TAK1 axis, and triggers NF-κB-dependent T-cell activation, thus forming a positive feedback loop of T-cell immunity in Nile tilapia. Furthermore, IL-17A seems to promote innate immunity by regulating pro-inflammatory cytokines via TRAF6-NF-κB axis, indicating the presence of an NF-κB-dependent IL-17A signaling pathway for coordinating adaptive and innate immunity in fish. Our results suggest that fish NF-κB couples TCR and IL-17 signals to modulate ancestral T-cell immunity against bacterial infection, and the regulation of T-cell immunity by NF-κB and IL-17 is a strategy that existed prior to the divergence of the tetrapod lineage from teleost fish. This study, therefore, provides a new perspective on the evolution of adaptive immunity.

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“…On the other hand, polyI:C is a double-stranded RNA analog that is frequently used as a virus simulator in scientific research. After the stimulation by LPS, the transcriptional expression of IL-17 family genes was quickly triggered in the Pacific oyster C. gigas and pearl oyster P. fucata [ 16 , 17 ]. Also in pearl oyster, Pf IL-17 was found to be involved in the immune response to polyI:C stimulation [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Role of a Novel Interleukin-17 Homolog from Invertebrate Marine Mussel Mytilus coruscus in Innate Immune Response: Is Negative Regulation by Mc-Novel_miR_145 the Key?

Chen

Qiu

et al. 2023

IJMS

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Interleukin-17 (IL-17) represents a class of proinflammatory cytokines involved in chronic inflammatory and degenerative disorders. Prior to this study, it was predicted that an IL-17 homolog could be targeted by Mc-novel_miR_145 to participate in the immune response of Mytilus coruscus. This study employed a variety of molecular and cell biology research methods to explore the association between Mc-novel_miR_145 and IL-17 homolog and their immunomodulatory effects. The bioinformatics prediction confirmed the affiliation of the IL-17 homolog with the mussel IL-17 family, followed by quantitative real-time PCR assays (qPCR) to demonstrate that McIL-17-3 was highly expressed in immune-associated tissues and responded to bacterial challenges. Results from luciferase reporter assays confirmed the potential of McIL-17-3 to activate downstream NF-κb and its targeting by Mc-novel_miR_145 in HEK293 cells. The study also produced McIL-17-3 antiserum and found that Mc-novel_miR_145 negatively regulates McIL-17-3 via western blotting and qPCR assays. Furthermore, flow cytometry analysis indicated that Mc-novel_miR_145 negatively regulated McIL-17-3 to alleviate LPS-induced apoptosis. Collectively, the current results showed that McIL-17-3 played an important role in molluscan immune defense against bacterial attack. Furthermore, McIL-17-3 was negatively regulated by Mc-novel_miR_145 to participate in LPS-induced apoptosis. Our findings provide new insights into noncoding RNA regulation in invertebrate models.

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AP-1 regulates the expression of IL17-4 and IL17-5 in the pacific oyster Crassostrea gigas

Cited by 19 publications

References 59 publications

Activator protein‐1 mediated CYP6ER1 overexpression in the clothianidin resistance of Nilaparvata lugens (Stål)

Activator protein‐1 mediated CYP6ER1 overexpression in the clothianidin resistance of Nilaparvata lugens (Stål)

Fish NF‐κB couples TCR and IL‐17 signals to regulate ancestral T‐cell immune response against bacterial infection

Exploring the Role of a Novel Interleukin-17 Homolog from Invertebrate Marine Mussel Mytilus coruscus in Innate Immune Response: Is Negative Regulation by Mc-Novel_miR_145 the Key?

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