Aortic remodelling during hemogenesis: is the chicken paradigm unique?

Jaffredo, Thierry; Richard, Charlotte; Pouget, Claire; Teillet, Marie-Aimée; Bollérot, Karine; Gautier, Rodolphe; Drevon, C.

doi:10.1387/ijdb.103062tj

Cited by 17 publications

(19 citation statements)

References 77 publications

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“…; for a review, see Jaffredo et al . ). Therefore, the somite is the definitive origin of dorsal aorta ECs.…”

Section: The Two Different Origins Of Dorsal Aorta Endothelial Cellsmentioning

confidence: 97%

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Dorsal aorta formation: Separate origins, lateral‐to‐medial migration, and remodeling

Sato

2012

Dev Growth Differ

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Blood vessel formation is a highly dynamic tissue-remodeling event that can be observed from early development in vertebrate embryos. Dorsal aortae, the first functional intra-embryonic blood vessels, arise as two separate bilateral vessels in the trunk and undergo lateral-to-medial translocation, eventually fusing into a single large vessel at the midline. After this dramatic remodeling, the dorsal aorta generates hematopoietic stem cells. The dorsal aorta is a good model to use to increase our understanding of the mechanisms controlling the establishment and remodeling of larger blood vessels in vivo. Because of the easy accessibility to the developing circulatory system, quail and chick embryos have been widely used for studies on blood vessel formation. In particular, the mapping of endothelial cell origins has been performed using quail-chick chimera analysis, revealing endothelial, vascular smooth muscle, and hematopoietic cell progenitors of the dorsal aorta. The avian embryo model also allows conditional gene activation/inactivation and direct observation of cell behaviors during dorsal aorta formation. This allows a better understanding of the molecular mechanisms underlying specific morphogenetic events during dynamic dorsal aorta formation from a cell behavior perspective.

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“…; for a review, see Jaffredo et al . ). Therefore, the somite is the definitive origin of dorsal aorta ECs.…”

Section: The Two Different Origins Of Dorsal Aorta Endothelial Cellsmentioning

confidence: 97%

“…; Jaffredo et al . ). Pulse labeling of ECs in a tamoxifen‐inducible system in mouse embryos confirmed that hematopoietic stem cells are derived from the ECs located at the dorsal aorta floor, similar to that observed in avian embryos (Zovein et al .…”

Section: Endothelial‐to‐hematopoietic Transition Of the Dorsal Aorta mentioning

confidence: 97%

Dorsal aorta formation: Separate origins, lateral‐to‐medial migration, and remodeling

Sato

2012

Dev Growth Differ

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“…In the embryo definitive HE can be identified based on anatomical location, morphology, and expression of Runx1 (Jaffredo et al, 2010; North et al, 1999; North et al, 2002). Because these criteria cannot be entirely applied to cells differentiated in vitro, the precise identification of HE in hPSC cultures remained as a significant challenge.…”

Section: Introductionmentioning

confidence: 99%

Identification of the Hemogenic Endothelial Progenitor and Its Direct Precursor in Human Pluripotent Stem Cell Differentiation Cultures

et al. 2012

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Summary Hemogenic endothelium (HE) has been recognized as a source of hematopoietic stem cells (HSCs) in the embryo. Access to human HE progenitors (HEPs) is essential to enable the investigation of the molecular determinants of HSC specification. Here we show that HEPs capable of generating definitive hematopoietic cells can be obtained from human pluripotent stem cells (hPSCs) and identified precisely by VE-cadherin+CD73−CD235a/CD43− phenotype. This phenotype discriminates true HEPs from VE-cadherin+CD73+ non-HEPs, and VE-cadherin+CD235a+CD41a− early hematopoietic cells with endothelial and FGF2-dependent hematopoietic colony-forming potential. We found that HEPs arise at the post primitive streak stage of differentiation directly from VE-cadherin-negative KDRbrightAPLNR+PDGFRαlow/− hematovascular mesodermal precursors (HVMPs). In contrast, hemangioblasts, which are capable of forming endothelium and primitive blood cells, originate from more immature APLNR+PDGFRα+ mesoderm. The demarcation of HEPs and HVMPs provides a platform for modeling blood development from endothelium with a goal to facilitate generation of HSCs from hPSCs.

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“…Considering that aortic hematopoiesis mostly originates from hemogenic ECs, it can be viewed as a cell fate change in which ECs loose their characteristics and acquire hematopoietic-specific markers (Jaffredo et al, 2010). This endothelial-to-hematopoietic transition is under the control of the Notch pathway.…”

Section: Introductionmentioning

confidence: 99%

Endothelio-Mesenchymal Interaction Controls runx1 Expression and Modulates the notch Pathway to Initiate Aortic Hematopoiesis

Richard¹,

Drevon²,

Canto³

et al. 2013

Developmental Cell

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SUMMARY Hematopoietic stem cells (HSCs) are produced by a small cohort of hemogenic endothelial cells (ECs) during development through the formation of intra-aortic hematopoietic cell (HC) clusters (HCs). The Runx1 transcription factor plays a key role in the EC to HC and HSC transition. We show that Runx1 expression in hemogenic ECs and the subsequent initiation of HC formation are tightly controlled by the sub-aortic mesenchyme, although the mesenchyme is not a source of HCs. Runx1 and Notch signaling are involved in this process, with Notch signaling decreasing with time in HCs. Inhibiting Notch signaling readily increases HC production in mouse and chicken embryos. In the mouse however, this increase is transient. Collectively, we show complementary roles of hemogenic ECs and mesenchymal compartments in triggering aortic hematopoiesis. The sub-aortic mesenchyme induces Runx1 expression in hemogenic-primed endothelial cells and collaborates with Notch dynamics to control aortic hematopoiesis.

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Aortic remodelling during hemogenesis: is the chicken paradigm unique?

Cited by 17 publications

References 77 publications

Dorsal aorta formation: Separate origins, lateral‐to‐medial migration, and remodeling

Dorsal aorta formation: Separate origins, lateral‐to‐medial migration, and remodeling

Identification of the Hemogenic Endothelial Progenitor and Its Direct Precursor in Human Pluripotent Stem Cell Differentiation Cultures

Endothelio-Mesenchymal Interaction Controls runx1 Expression and Modulates the notch Pathway to Initiate Aortic Hematopoiesis

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